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Inflammation and remodelling patterns in early stage chronic rhinosinusitis

NICHOLAS VAN BRUAENE UGent, Claudina Perez Novo UGent, Koen Van Crombruggen UGent, Natalie De Ruyck UGent, Gabriële Holtappels UGent, Paul Van Cauwenberge UGent, Philippe Gevaert UGent and Claus Bachert UGent (2012) CLINICAL AND EXPERIMENTAL ALLERGY. 42(6). p.883-890
abstract
Background A distinct set of inflammatory and remodelling factors have been found elevated in chronic rhinosinusitis. Objective The investigation of their expression in early stage disease may reveal early events in this common disease. Methods Sinonasal mucosal samples from nine patients with early stage CRSsNP were taken from the inferior and middle turbinates, the uncinate process, maxillary sinus, anterior ethmoid, bulla ethmoidalis and the posterior ethmoid and measured for TGF-beta 1 and it's receptors, MPO protein as well as pro-inflammatory cytokines (TNF-alpha and IL-1beta) and the Th1 cell signature (IFN-gamma and T-bet). As outcome parameter for TGF-beta signalling collagen deposition was analysed. Inferior turbinates from patients undergoing (rhino-) septoplasty were collected as controls. Results TGF-beta 1 protein concentrations were significantly increased in the maxillary sinuses (P = 0.006), the uncinate process (P = 0.01), the anterior ethmoid including the bulla ethmoidalis (P = 0.005) and the posterior ethmoid (P = 0.037) when compared to the inferior and middle turbinates. Collagen deposition was significantly increased in the maxillary sinus when compared to the inferior turbinates (P = 0.008). In contrast, mRNA for TGF-beta receptors, Th1 related markers (IFN-gamma and T-bet), pro-inflammatory cytokines (IL-1 beta and TNF-alpha), and MPO protein as neutrophil marker were expressed at all locations but showed no significant differences between the various locations. TGF-beta 1 mRNA expression in inferior turbinates of CRSsNP was significantly higher when compared to inferior turbinates of controls (P = 0.017). The pro-inflammatory cytokines and Th1-related cytokines did not show an upregulation in inferior turbinates of CRSsNP when compared to controls. Conclusions In early stage chronic sinus disease, TGF-beta protein is expressed in significantly higher concentrations within the paranasal sinuses when compared to turbinates, whereas pro-inflammatory, neutrophilic and Th1 markers did not show any difference. These findings suggest that TGF-beta plays a central role in the initiation of CRSsNP, and represents a major target for further research and future intervention.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
chronic rhinosinusitis, Turbinates, inflammation, remodelling, TGF-beta, SINUS DISEASE, ASTHMA, PATHOPHYSIOLOGY
journal title
CLINICAL AND EXPERIMENTAL ALLERGY
Clin. Exp. Allergy
volume
42
issue
6
pages
883 - 890
Web of Science type
Article
Web of Science id
000305000800007
JCR category
ALLERGY
JCR impact factor
4.789 (2012)
JCR rank
4/23 (2012)
JCR quartile
1 (2012)
ISSN
0954-7894
DOI
10.1111/j.1365-2222.2011.03898.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1987810
handle
http://hdl.handle.net/1854/LU-1987810
date created
2012-01-16 20:03:34
date last changed
2012-09-21 16:13:02
@article{1987810,
  abstract     = {Background A distinct set of inflammatory and remodelling factors have been found elevated in chronic rhinosinusitis. 
Objective The investigation of their expression in early stage disease may reveal early events in this common disease. 
Methods Sinonasal mucosal samples from nine patients with early stage CRSsNP were taken from the inferior and middle turbinates, the uncinate process, maxillary sinus, anterior ethmoid, bulla ethmoidalis and the posterior ethmoid and measured for TGF-beta 1 and it's receptors, MPO protein as well as pro-inflammatory cytokines (TNF-alpha and IL-1beta) and the Th1 cell signature (IFN-gamma and T-bet). As outcome parameter for TGF-beta signalling collagen deposition was analysed. Inferior turbinates from patients undergoing (rhino-) septoplasty were collected as controls. 
Results TGF-beta 1 protein concentrations were significantly increased in the maxillary sinuses (P = 0.006), the uncinate process (P = 0.01), the anterior ethmoid including the bulla ethmoidalis (P = 0.005) and the posterior ethmoid (P = 0.037) when compared to the inferior and middle turbinates. Collagen deposition was significantly increased in the maxillary sinus when compared to the inferior turbinates (P = 0.008). In contrast, mRNA for TGF-beta receptors, Th1 related markers (IFN-gamma and T-bet), pro-inflammatory cytokines (IL-1 beta and TNF-alpha), and MPO protein as neutrophil marker were expressed at all locations but showed no significant differences between the various locations. TGF-beta 1 mRNA expression in inferior turbinates of CRSsNP was significantly higher when compared to inferior turbinates of controls (P = 0.017). The pro-inflammatory cytokines and Th1-related cytokines did not show an upregulation in inferior turbinates of CRSsNP when compared to controls. 
Conclusions In early stage chronic sinus disease, TGF-beta protein is expressed in significantly higher concentrations within the paranasal sinuses when compared to turbinates, whereas pro-inflammatory, neutrophilic and Th1 markers did not show any difference. These findings suggest that TGF-beta plays a central role in the initiation of CRSsNP, and represents a major target for further research and future intervention.},
  author       = {VAN BRUAENE, NICHOLAS and Perez Novo, Claudina and Van Crombruggen, Koen and De Ruyck, Natalie and Holtappels, Gabri{\"e}le and Van Cauwenberge, Paul and Gevaert, Philippe and Bachert, Claus},
  issn         = {0954-7894},
  journal      = {CLINICAL AND EXPERIMENTAL ALLERGY},
  keyword      = {chronic rhinosinusitis,Turbinates,inflammation,remodelling,TGF-beta,SINUS DISEASE,ASTHMA,PATHOPHYSIOLOGY},
  language     = {eng},
  number       = {6},
  pages        = {883--890},
  title        = {Inflammation and remodelling patterns in early stage chronic rhinosinusitis},
  url          = {http://dx.doi.org/10.1111/j.1365-2222.2011.03898.x},
  volume       = {42},
  year         = {2012},
}

Chicago
VAN BRUAENE, NICHOLAS, Claudina Perez Novo, Koen Van Crombruggen, Natalie De Ruyck, Gabriële Holtappels, Paul Van Cauwenberge, Philippe Gevaert, and Claus Bachert. 2012. “Inflammation and Remodelling Patterns in Early Stage Chronic Rhinosinusitis.” Clinical and Experimental Allergy 42 (6): 883–890.
APA
VAN BRUAENE, N., Perez Novo, C., Van Crombruggen, K., De Ruyck, N., Holtappels, G., Van Cauwenberge, P., Gevaert, P., et al. (2012). Inflammation and remodelling patterns in early stage chronic rhinosinusitis. CLINICAL AND EXPERIMENTAL ALLERGY, 42(6), 883–890.
Vancouver
1.
VAN BRUAENE N, Perez Novo C, Van Crombruggen K, De Ruyck N, Holtappels G, Van Cauwenberge P, et al. Inflammation and remodelling patterns in early stage chronic rhinosinusitis. CLINICAL AND EXPERIMENTAL ALLERGY. 2012;42(6):883–90.
MLA
VAN BRUAENE, NICHOLAS, Claudina Perez Novo, Koen Van Crombruggen, et al. “Inflammation and Remodelling Patterns in Early Stage Chronic Rhinosinusitis.” CLINICAL AND EXPERIMENTAL ALLERGY 42.6 (2012): 883–890. Print.