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Decellularization of heart valve matrices: search for the ideal balance

Pamela Somers UGent, Filip De Somer UGent, Maria Cornelissen UGent, Hubert Thierens UGent and Guido Van Nooten UGent (2012) ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY. 40(1-2). p.151-162
abstract
Objective: Currently used decellularization procedures have negative effects on extracellular matrix (ECM) integrity. The objective of this study is to evaluate four decellularization methods and their effect on the collagen ultrastructure, mechanical behavior and antigenicity of porcine aortic valves. Methods: Aortic valves were placed in a trypsin, osmotic, trypsin-osmotic or detergent-osmotic solution. Leaflets were processed for histology and mechanical testing. Matrices were implanted subdermally in rats to evaluate immune reaction and calcification. Results: Trypsin-osmotic methodology effected near-complete decellularization. Trypsin treatment resulted in cell removal only in the spongiosa layer. Osmotic and detergent-osmotic treatments did not remove any cells from the cusps. Mechanical strength was significantly inferior in the trypsin (p = 0,03) and trypsin-osmotic treated group (p = 0,04). Trypsin and trypsin-osmotic decellularized matrices evoked a strong CD3+ inflammatory cell infiltration. Conclusion: Enzymatic-osmotic decellularization appears to be the only effective method to remove cellular components. However, the near cell free scaffolds still evokes a strong CD3+ T-cell inflammatory reaction.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Decellularization, Heart valve, Immune reaction, Glycosaminoglycans, Tissue engineering, AORTIC VALVES, TISSUE, PORCINE, PROTEOGLYCANS, SCAFFOLDS, MECHANISM, CUSPS, CELLS
journal title
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
Artif. Cells Blood Substit. Biotechnol.
volume
40
issue
1-2
pages
151 - 162
Web of Science type
Article
Web of Science id
000299696500020
JCR category
ENGINEERING, BIOMEDICAL
JCR impact factor
0.854 (2012)
JCR rank
62/77 (2012)
JCR quartile
4 (2012)
ISSN
1073-1199
DOI
10.3109/10731199.2011.637925
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1986960
handle
http://hdl.handle.net/1854/LU-1986960
date created
2012-01-16 11:12:51
date last changed
2012-02-27 12:35:13
@article{1986960,
  abstract     = {Objective: Currently used decellularization procedures have negative effects on extracellular matrix (ECM) integrity. The objective of this study is to evaluate four decellularization methods and their effect on the collagen ultrastructure, mechanical behavior and antigenicity of porcine aortic valves. Methods: Aortic valves were placed in a trypsin, osmotic, trypsin-osmotic or detergent-osmotic solution. Leaflets were processed for histology and mechanical testing. Matrices were implanted subdermally in rats to evaluate immune reaction and calcification. Results: Trypsin-osmotic methodology effected near-complete decellularization. Trypsin treatment resulted in cell removal only in the spongiosa layer. Osmotic and detergent-osmotic treatments did not remove any cells from the cusps. Mechanical strength was significantly inferior in the trypsin (p = 0,03) and trypsin-osmotic treated group (p = 0,04). Trypsin and trypsin-osmotic decellularized matrices evoked a strong CD3+ inflammatory cell infiltration. Conclusion: Enzymatic-osmotic decellularization appears to be the only effective method to remove cellular components. However, the near cell free scaffolds still evokes a strong CD3+ T-cell inflammatory reaction.},
  author       = {Somers, Pamela and De Somer, Filip and Cornelissen, Maria and Thierens, Hubert and Van Nooten, Guido},
  issn         = {1073-1199},
  journal      = {ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY},
  keyword      = {Decellularization,Heart valve,Immune reaction,Glycosaminoglycans,Tissue engineering,AORTIC VALVES,TISSUE,PORCINE,PROTEOGLYCANS,SCAFFOLDS,MECHANISM,CUSPS,CELLS},
  language     = {eng},
  number       = {1-2},
  pages        = {151--162},
  title        = {Decellularization of heart valve matrices: search for the ideal balance},
  url          = {http://dx.doi.org/10.3109/10731199.2011.637925},
  volume       = {40},
  year         = {2012},
}

Chicago
Somers, Pamela, Filip De Somer, Maria Cornelissen, Hubert Thierens, and Guido Van Nooten. 2012. “Decellularization of Heart Valve Matrices: Search for the Ideal Balance.” Artificial Cells Blood Substitutes and Biotechnology 40 (1-2): 151–162.
APA
Somers, P., De Somer, F., Cornelissen, M., Thierens, H., & Van Nooten, G. (2012). Decellularization of heart valve matrices: search for the ideal balance. ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, 40(1-2), 151–162.
Vancouver
1.
Somers P, De Somer F, Cornelissen M, Thierens H, Van Nooten G. Decellularization of heart valve matrices: search for the ideal balance. ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY. 2012;40(1-2):151–62.
MLA
Somers, Pamela, Filip De Somer, Maria Cornelissen, et al. “Decellularization of Heart Valve Matrices: Search for the Ideal Balance.” ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY 40.1-2 (2012): 151–162. Print.