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An optimized growth factor cocktail for ovine mesenchymal stem cells

Pamela Somers UGent, Maria Cornelissen UGent, Hubert Thierens UGent and Guido Van Nooten UGent (2012) GROWTH FACTORS. 30(1). p.37-48
abstract
Growth factors that regulate proliferation, migration, and invasion of ovine mesenchymal stem cells (oMSCs) are not well defined. In this study, we have evaluated five growth factors for their ability to initiate and support in vitro proliferation, migration, and invasion of oMSCs. oMSCs were exposed to different doses and combinations of the growth factors: basic fibroblast growth factor (bFGF), transforming growth factor-beta (TGF-beta), epidermal growth factor (EGF), insulin growth factor-I (IGF-I), connective tissue growth factor, and platelet-derived growth factor-AB (PDGF-AB). Cellular proliferation, motility, and invasiveness were assayed. The most proliferative stimulating growth factors are PDGF-AB + TGF-beta and PDGF-AB + IGF-I. Combinations EGF + bFGF and EGF + bFGF + PDGF-AB demonstrated the greatest ability to stimulate migration. Moreover, the triple cocktail EGF + bFGF + TGF-beta has the most significant effect on invasion. Different growth factor cocktails are required to enhance proliferation, migration, and invasion. These results may be useful for the development of a tissue-engineered heart valve by stimulating cellular repopulation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Mesenchymal stem cells, tissue engineering, growth factors, MYOCARDIAL-INFARCTION, STROMAL CELLS, DIFFERENTIATION, SCAFFOLDS, ISCHEMIA, THERAPY, MODEL, PROGENITOR CELLS, HEART-VALVE, HUMAN BONE-MARROW, invasion, migration, proliferation
journal title
GROWTH FACTORS
Growth Factors
volume
30
issue
1
pages
37 - 48
Web of Science type
Article
Web of Science id
000299053800005
JCR category
ENDOCRINOLOGY & METABOLISM
JCR impact factor
2.195 (2012)
JCR rank
75/121 (2012)
JCR quartile
3 (2012)
ISSN
0897-7194
DOI
10.3109/08977194.2011.634411
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1986954
handle
http://hdl.handle.net/1854/LU-1986954
date created
2012-01-16 11:08:11
date last changed
2012-09-20 14:00:53
@article{1986954,
  abstract     = {Growth factors that regulate proliferation, migration, and invasion of ovine mesenchymal stem cells (oMSCs) are not well defined. In this study, we have evaluated five growth factors for their ability to initiate and support in vitro proliferation, migration, and invasion of oMSCs. oMSCs were exposed to different doses and combinations of the growth factors: basic fibroblast growth factor (bFGF), transforming growth factor-beta (TGF-beta), epidermal growth factor (EGF), insulin growth factor-I (IGF-I), connective tissue growth factor, and platelet-derived growth factor-AB (PDGF-AB). Cellular proliferation, motility, and invasiveness were assayed. The most proliferative stimulating growth factors are PDGF-AB + TGF-beta and PDGF-AB + IGF-I. Combinations EGF + bFGF and EGF + bFGF + PDGF-AB demonstrated the greatest ability to stimulate migration. Moreover, the triple cocktail EGF + bFGF + TGF-beta has the most significant effect on invasion. Different growth factor cocktails are required to enhance proliferation, migration, and invasion. These results may be useful for the development of a tissue-engineered heart valve by stimulating cellular repopulation.},
  author       = {Somers, Pamela and Cornelissen, Maria and Thierens, Hubert and Van Nooten, Guido},
  issn         = {0897-7194},
  journal      = {GROWTH FACTORS},
  keyword      = {Mesenchymal stem cells,tissue engineering,growth factors,MYOCARDIAL-INFARCTION,STROMAL CELLS,DIFFERENTIATION,SCAFFOLDS,ISCHEMIA,THERAPY,MODEL,PROGENITOR CELLS,HEART-VALVE,HUMAN BONE-MARROW,invasion,migration,proliferation},
  language     = {eng},
  number       = {1},
  pages        = {37--48},
  title        = {An optimized growth factor cocktail for ovine mesenchymal stem cells},
  url          = {http://dx.doi.org/10.3109/08977194.2011.634411},
  volume       = {30},
  year         = {2012},
}

Chicago
Somers, Pamela, Maria Cornelissen, Hubert Thierens, and Guido Van Nooten. 2012. “An Optimized Growth Factor Cocktail for Ovine Mesenchymal Stem Cells.” Growth Factors 30 (1): 37–48.
APA
Somers, P., Cornelissen, M., Thierens, H., & Van Nooten, G. (2012). An optimized growth factor cocktail for ovine mesenchymal stem cells. GROWTH FACTORS, 30(1), 37–48.
Vancouver
1.
Somers P, Cornelissen M, Thierens H, Van Nooten G. An optimized growth factor cocktail for ovine mesenchymal stem cells. GROWTH FACTORS. 2012;30(1):37–48.
MLA
Somers, Pamela, Maria Cornelissen, Hubert Thierens, et al. “An Optimized Growth Factor Cocktail for Ovine Mesenchymal Stem Cells.” GROWTH FACTORS 30.1 (2012): 37–48. Print.