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Resistance of red clover to broad spectrum of Sclerotinia trifoliorum

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Abstract
In this research a diversity study on different European isolates of the pathogenic fungus S. trifoliorum will be performed using mycelial compatibility grouping and AFLP. The next step is the development of a bio-test to screen red clover plants for their resistance level against clover rot. The third step in this research is evaluating different European strains for their virulence and evaluating a broad spectrum of red clover varieties for their resistance against S. trifoliorum. Over 100 varieties will be evaluated, including cultivars, landraces and wild varieties. Finally the inheritage of clover rot resistance in red clover will be evaluated by a QTL study. Sclerotinia isolates have been collected from clover fields among different European countries. Mycelial compatibility has shown a large variability within fields. The DNA extraction has been optimized. Sequencing of the ITS-region will be used to determine the exact species of every isolate. Primer combinations are currently being tested for the AFLP study. Different culture media were tested for their capacity to induce the production of multiple big sclerotia. The most optimal medium is being used to produce sclerotia from every isolate. Sclerotia are induced to apothecia formation and formed ascospores will be used to construct the bio-test.
Keywords
AFLP, Bio-test, Diversity study, Trifolium pratense, Sclerotinia trifoliorum

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Citation

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Chicago
Vleugels, Tim, Joost Baert, Kurt Heungens, Marianne Malengier, Gerda Cnops, and Erik Van Bockstaele. 2010. “Resistance of Red Clover to Broad Spectrum of Sclerotinia Trifoliorum.” In Sustainable Use of Genetic Diversity in Forage and Turf Breeding, ed. Christian Huyghe, 375–380. Berlin, Germany: Springer.
APA
Vleugels, Tim, Baert, J., Heungens, K., Malengier, M., Cnops, G., & Van Bockstaele, E. (2010). Resistance of red clover to broad spectrum of Sclerotinia trifoliorum. In Christian Huyghe (Ed.), Sustainable use of genetic diversity in forage and turf breeding (pp. 375–380). Presented at the Conference of the Eucarpia Fodder and Amenity Species Section, Berlin, Germany: Springer.
Vancouver
1.
Vleugels T, Baert J, Heungens K, Malengier M, Cnops G, Van Bockstaele E. Resistance of red clover to broad spectrum of Sclerotinia trifoliorum. In: Huyghe C, editor. Sustainable use of genetic diversity in forage and turf breeding. Berlin, Germany: Springer; 2010. p. 375–80.
MLA
Vleugels, Tim, Joost Baert, Kurt Heungens, et al. “Resistance of Red Clover to Broad Spectrum of Sclerotinia Trifoliorum.” Sustainable Use of Genetic Diversity in Forage and Turf Breeding. Ed. Christian Huyghe. Berlin, Germany: Springer, 2010. 375–380. Print.
@inproceedings{1986948,
  abstract     = {In this research a diversity study on different European isolates of the pathogenic fungus S. trifoliorum will be performed using mycelial compatibility grouping and AFLP. The next step is the development of a bio-test to screen red clover plants for their resistance level against clover rot. The third step in this research is evaluating different European strains for their virulence and evaluating a broad spectrum of red clover varieties for their resistance against S. trifoliorum. Over 100 varieties will be evaluated, including cultivars, landraces and wild varieties. Finally the inheritage of clover rot resistance in red clover will be evaluated by a QTL study. 
Sclerotinia isolates have been collected from clover fields among different European countries. Mycelial compatibility has shown a large variability within fields. The DNA extraction has been optimized. Sequencing of the ITS-region will be used to determine the exact species of every isolate. Primer combinations are currently being tested for the AFLP study. Different culture media were tested for their capacity to induce the production of multiple big sclerotia. The most optimal medium is being used to produce sclerotia from every isolate. Sclerotia are induced to apothecia formation and formed ascospores will be used to construct the bio-test.},
  author       = {Vleugels, Tim and Baert, Joost and Heungens, Kurt and Malengier, Marianne and Cnops, Gerda and Van Bockstaele, Erik},
  booktitle    = {Sustainable use of genetic diversity in forage and turf breeding},
  editor       = {Huyghe, Christian},
  isbn         = {9789048187058},
  keyword      = {AFLP,Bio-test,Diversity study,Trifolium pratense,Sclerotinia trifoliorum},
  language     = {eng},
  location     = {La Rochelle, France},
  pages        = {375--380},
  publisher    = {Springer},
  title        = {Resistance of red clover to broad spectrum of Sclerotinia trifoliorum},
  url          = {http://dx.doi.org/10.1007/978-90-481-8706-5\_54},
  year         = {2010},
}

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