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Whole organ, venation and epidermal cell morphological variations are correlated in the leaves of Arabidopsis mutants

(2011) PLANT CELL AND ENVIRONMENT. 34(12). p.2200-2211
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Abstract
Despite the large number of genes known to affect leaf shape or size, we still have a relatively poor understanding of how leaf morphology is established. For example, little is known about how cell division and cell expansion are controlled and coordinated within a growing leaf to eventually develop into a laminar organ of a definite size. To obtain a global perspective of the cellular basis of variations in leaf morphology at the organ, tissue and cell levels, we studied a collection of 111 non-allelic mutants with abnormally shaped and/or sized leaves, which broadly represent the mutational variations in Arabidopsis thaliana leaf morphology not associated with lethality. We used image-processing techniques on these mutants to quantify morphological parameters running the gamut from the palisade mesophyll and epidermal cells to the venation, whole leaf and rosette levels. We found positive correlations between epidermal cell size and leaf area, which is consistent with long-standing Avery's hypothesis that the epidermis drives leaf growth. In addition, venation parameters were positively correlated with leaf area, suggesting that leaf growth and vein patterning share some genetic controls. Positional cloning of the genes affected by the studied mutations will eventually establish functional links between genotypes, molecular functions, cellular parameters and leaf phenotypes.
Keywords
leaf epidermis, palisade mesophyll, GENETIC-ANALYSIS, phenomics, principal component analysis, venation pattern, GENOME-WIDE MUTAGENESIS, THALIANA VEGETATIVE LEAVES, LEAF-SHAPE, SIZE, MORPHOGENESIS, PROLIFERATION, ARCHITECTURE, EXPANSION, GROWTH

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Chicago
Pérez-Pérez, José Manuel, Silvia Rubio-Díaz, Stijn Dhondt, Diana Hernández-Romero, Joaquín Sánchez-Soriano, Gerrit Beemster, María Rosa Ponce, and José Luis Micol. 2011. “Whole Organ, Venation and Epidermal Cell Morphological Variations Are Correlated in the Leaves of Arabidopsis Mutants.” Plant Cell and Environment 34 (12): 2200–2211.
APA
Pérez-Pérez, J. M., Rubio-Díaz, S., Dhondt, S., Hernández-Romero, D., Sánchez-Soriano, J., Beemster, G., Ponce, M. R., et al. (2011). Whole organ, venation and epidermal cell morphological variations are correlated in the leaves of Arabidopsis mutants. PLANT CELL AND ENVIRONMENT, 34(12), 2200–2211.
Vancouver
1.
Pérez-Pérez JM, Rubio-Díaz S, Dhondt S, Hernández-Romero D, Sánchez-Soriano J, Beemster G, et al. Whole organ, venation and epidermal cell morphological variations are correlated in the leaves of Arabidopsis mutants. PLANT CELL AND ENVIRONMENT. 2011;34(12):2200–11.
MLA
Pérez-Pérez, José Manuel, Silvia Rubio-Díaz, Stijn Dhondt, et al. “Whole Organ, Venation and Epidermal Cell Morphological Variations Are Correlated in the Leaves of Arabidopsis Mutants.” PLANT CELL AND ENVIRONMENT 34.12 (2011): 2200–2211. Print.
@article{1986463,
  abstract     = {Despite the large number of genes known to affect leaf shape or size, we still have a relatively poor understanding of how leaf morphology is established. For example, little is known about how cell division and cell expansion are controlled and coordinated within a growing leaf to eventually develop into a laminar organ of a definite size. To obtain a global perspective of the cellular basis of variations in leaf morphology at the organ, tissue and cell levels, we studied a collection of 111 non-allelic mutants with abnormally shaped and/or sized leaves, which broadly represent the mutational variations in Arabidopsis thaliana leaf morphology not associated with lethality. We used image-processing techniques on these mutants to quantify morphological parameters running the gamut from the palisade mesophyll and epidermal cells to the venation, whole leaf and rosette levels. We found positive correlations between epidermal cell size and leaf area, which is consistent with long-standing Avery's hypothesis that the epidermis drives leaf growth. In addition, venation parameters were positively correlated with leaf area, suggesting that leaf growth and vein patterning share some genetic controls. Positional cloning of the genes affected by the studied mutations will eventually establish functional links between genotypes, molecular functions, cellular parameters and leaf phenotypes.},
  author       = {P{\'e}rez-P{\'e}rez, Jos{\'e} Manuel and Rubio-D{\'i}az, Silvia and Dhondt, Stijn and Hern{\'a}ndez-Romero, Diana and S{\'a}nchez-Soriano, Joaqu{\'i}n and Beemster, Gerrit and Ponce, Mar{\'i}a Rosa and Micol, Jos{\'e} Luis},
  issn         = {0140-7791},
  journal      = {PLANT CELL AND ENVIRONMENT},
  keyword      = {leaf epidermis,palisade mesophyll,GENETIC-ANALYSIS,phenomics,principal component analysis,venation pattern,GENOME-WIDE MUTAGENESIS,THALIANA VEGETATIVE LEAVES,LEAF-SHAPE,SIZE,MORPHOGENESIS,PROLIFERATION,ARCHITECTURE,EXPANSION,GROWTH},
  language     = {eng},
  number       = {12},
  pages        = {2200--2211},
  title        = {Whole organ, venation and epidermal cell morphological variations are correlated in the leaves of Arabidopsis mutants},
  url          = {http://dx.doi.org/10.1111/j.1365-3040.2011.02415.x},
  volume       = {34},
  year         = {2011},
}

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