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Clostridium perfringens strains of various origin can cause hemorrhagic enteritis in a calf intestinal loop model

Bonnie Valgaeren UGent, Evy Goossens UGent, Stefanie Verherstraeten UGent, Bart Pardon UGent, Leen Timbermont UGent, Stijn Schauvlieghe, Richard Ducatelle UGent, Filip Van Immerseel UGent and Piet Deprez UGent (2011) 7th International conference on the molecular biology and pathogenesis of the Clostridia : program and abstract book.
abstract
Hemorrhagic enteritis is one of numerous pathologies caused by Clostridium perfringens in cattle. It is an acute syndrome with a case fatality rate close to 100%, that affects mainly suckling and veal calves in good to excellent body condition up to four months of age. In Belgian Blue cattle, losses due to hemorrhagic enteritis may be responsible for up to 20% of total mortality. The pathogenesis and the toxins involved in bovine hemorrhagic enteritis remain to be elucidated. Therefore an intestinal loop model was developed to test a collection of Clostridium perfringens strains for their ability to reproduce the pathology. Loops were injected with logarithmic C. perfringens cultures in combination or not with commercial milk replacer for veal calves. The tested set of strains consisted of bovine strains isolated from both hemorrhagic enteritis cases and healthy animals, as well as human, NetB positive and negative chicken and Beta toxin positive porcine isolates. Also a VirR, α and θ mutant were tested. All tested strains were capable of eliciting hemorrhagic enteritis in this experimental setup when inoculated together with commercial milk replacer. A time course experiment showed that the pathogenesis is characterized by early capillary congestion, followed by hemorrhages and necrosis of the lamina propria, originating from the tips of the villi. No edema was present. C. perfringens bacteria were often attached to cellular debris in the lumen. These observations indicate that any Clostridium perfringens strain, independent of the isolation source, may be capable of eliciting hemorrhagic enteritis, and that this syndrome is not caused by host-specific strains or toxins. In addition, the pathological process seems to be independent of α- or θ-toxin or VirR-regulated mechanisms.
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author
organization
year
type
conference
publication status
published
subject
in
7th International conference on the molecular biology and pathogenesis of the Clostridia : program and abstract book
article_number
abstract P46
publisher
Iowa State University (ISU). College of Veterinary Medicine
place of publication
Ames, IA, USA
conference name
7th International conference on the Molecular Biology and Pathogenesis of the Clostridia (ClosPath 7)
conference location
Ames, Iowa USA
conference start
2011-10-25
conference end
2011-10-29
language
English
UGent publication?
yes
classification
C3
id
1985913
handle
http://hdl.handle.net/1854/LU-1985913
date created
2012-01-13 13:25:39
date last changed
2012-04-06 10:54:32
@inproceedings{1985913,
  abstract     = {Hemorrhagic enteritis is one of numerous pathologies caused by Clostridium perfringens in cattle. It is an acute syndrome with a case fatality rate close to 100\%, that affects mainly suckling and veal calves in good to excellent body condition up to four months of age. In Belgian Blue cattle, losses due to hemorrhagic enteritis may be responsible for up to 20\% of total mortality. The pathogenesis and the toxins involved in bovine hemorrhagic enteritis remain to be elucidated. 
Therefore an intestinal loop model was developed to test a collection of Clostridium perfringens strains for their ability to reproduce the pathology. Loops were injected with logarithmic C. perfringens cultures in combination or not with commercial milk replacer for veal calves. The tested set of strains consisted of bovine strains isolated from both hemorrhagic enteritis cases and healthy animals, as well as human, NetB positive and negative chicken and Beta toxin positive porcine isolates. Also a VirR, \ensuremath{\alpha} and \ensuremath{\theta} mutant were tested.
All tested strains were capable of eliciting hemorrhagic enteritis in this experimental setup when inoculated together with commercial milk replacer. A time course experiment showed that the pathogenesis is characterized by early capillary congestion, followed by hemorrhages and necrosis of the lamina propria, originating from the tips of the villi. No edema was present. C. perfringens bacteria were often attached to cellular debris in the lumen.
These observations indicate that any Clostridium perfringens strain, independent of the isolation source, may be capable of eliciting hemorrhagic enteritis, and that this syndrome is not caused by host-specific strains or toxins. In addition, the pathological process seems to be independent of \ensuremath{\alpha}- or \ensuremath{\theta}-toxin or VirR-regulated mechanisms.},
  articleno    = {abstract P46},
  author       = {Valgaeren, Bonnie and Goossens, Evy and Verherstraeten, Stefanie and Pardon, Bart and Timbermont, Leen and Schauvlieghe, Stijn and Ducatelle, Richard and Van Immerseel, Filip and Deprez, Piet},
  booktitle    = {7th International conference on the molecular biology and pathogenesis of the Clostridia : program and abstract book},
  language     = {eng},
  location     = {Ames, Iowa USA},
  publisher    = {Iowa State University (ISU). College of Veterinary Medicine},
  title        = {Clostridium perfringens strains of various origin can cause hemorrhagic enteritis in a calf intestinal loop model},
  year         = {2011},
}

Chicago
Valgaeren, Bonnie, Evy Goossens, Stefanie Verherstraeten, Bart Pardon, Leen Timbermont, Stijn Schauvlieghe, Richard Ducatelle, Filip Van Immerseel, and Piet Deprez. 2011. “Clostridium Perfringens Strains of Various Origin Can Cause Hemorrhagic Enteritis in a Calf Intestinal Loop Model.” In 7th International Conference on the Molecular Biology and Pathogenesis of the Clostridia : Program and Abstract Book. Ames, IA, USA: Iowa State University (ISU). College of Veterinary Medicine.
APA
Valgaeren, B., Goossens, E., Verherstraeten, S., Pardon, B., Timbermont, L., Schauvlieghe, S., Ducatelle, R., et al. (2011). Clostridium perfringens strains of various origin can cause hemorrhagic enteritis in a calf intestinal loop model. 7th International conference on the molecular biology and pathogenesis of the Clostridia : program and abstract book. Presented at the 7th International conference on the Molecular Biology and Pathogenesis of the Clostridia (ClosPath 7), Ames, IA, USA: Iowa State University (ISU). College of Veterinary Medicine.
Vancouver
1.
Valgaeren B, Goossens E, Verherstraeten S, Pardon B, Timbermont L, Schauvlieghe S, et al. Clostridium perfringens strains of various origin can cause hemorrhagic enteritis in a calf intestinal loop model. 7th International conference on the molecular biology and pathogenesis of the Clostridia : program and abstract book. Ames, IA, USA: Iowa State University (ISU). College of Veterinary Medicine; 2011.
MLA
Valgaeren, Bonnie, Evy Goossens, Stefanie Verherstraeten, et al. “Clostridium Perfringens Strains of Various Origin Can Cause Hemorrhagic Enteritis in a Calf Intestinal Loop Model.” 7th International Conference on the Molecular Biology and Pathogenesis of the Clostridia : Program and Abstract Book. Ames, IA, USA: Iowa State University (ISU). College of Veterinary Medicine, 2011. Print.