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Peroxisomes in liver, heart, and kidney of mice fed a commercial fish oil preparation: original data and review on peroxisomal changes induced by high-fat diets

Dirk De Craemer UGent, Joseph Vamecq, Frank Roels, Louis Vallée, Marina Pauwels and Christiane Van den Branden (1994) JOURNAL OF LIPID RESEARCH. 35(7). p.1241-1250
abstract
Male NMRI mice were fed a diet with 10% w/w Beromegan(R) for up to three weeks. Beromegan(R) is a commercial fish (salmon) oil preparation rich in eicosapentaenoic acid and docosahexaenoic acid. Peroxisomal beta-oxidation capacity, catalase activity, and ultrastructural morphometry of the hepatic peroxisomes were investigated. In myocardium and kidney, catalase activity, peroxisomal staining after catalase cytochemistry, peroxisomal morphology, and morphometry (in myocardium) were evaluated. In liver, we found a significant increase in peroxisomal beta-oxidation, catalase activity, and peroxisomal number already after 3 days of dietary treatment. These changes were more pronounced after 3 weeks. Peroxisomal size was not changed. Positive correlations were found between peroxisomal enzyme activities and the number but not the size of the peroxisomes, and between catalase activity and beta-oxidation capacity. The mean peroxisomal diameter per animal was inversely proportional to catalase activity measured in homogenate. In myocardium, catalase activity was increased with duration of fish oil feeding. Peroxisomal staining, number, and size were also increased when compared to controls. In kidney, no alterations were observed. Our results indicate a beneficial effect of a diet supplemented with fish oil on the peroxisomal metabolism in liver and myocardium; it differs from the changes induced by xenobiotic peroxisome proliferation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CATALASE CYTOCHEMISTRY, CATALASE ACTIVITY, MORPHOMETRY, DOCOSAHEXAENOIC ACID, POLYUNSATURATED FATTY ACIDS, PEROXISOMAL BETA-OXIDATION, MITOCHONDRIAL BETA-OXIDATION, RAT-LIVER, ACID OXIDATION, EICOSAPENTAENOIC ACID, HEPATOCELLULAR PEROXISOMES, HEPATIC PEROXISOMES, CLOFIBRIC ACID, ERUCIC-ACID, METABOLISM, ADRENOLEUKODYSTROPHY
journal title
JOURNAL OF LIPID RESEARCH
J. Lipid Res.
volume
35
issue
7
pages
1241-1250 pages
Web of Science type
Article
ISSN
0022-2275
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
198577
handle
http://hdl.handle.net/1854/LU-198577
alternative location
http://www.jlr.org/cgi/content/abstract/35/7/1241
date created
2004-01-14 13:42:00
date last changed
2016-12-19 15:38:11
@article{198577,
  abstract     = {Male NMRI mice were fed a diet with 10\% w/w Beromegan(R) for up to three weeks. Beromegan(R) is a commercial fish (salmon) oil preparation rich in eicosapentaenoic acid and docosahexaenoic acid. Peroxisomal beta-oxidation capacity, catalase activity, and ultrastructural morphometry of the hepatic peroxisomes were investigated. In myocardium and kidney, catalase activity, peroxisomal staining after catalase cytochemistry, peroxisomal morphology, and morphometry (in myocardium) were evaluated. In liver, we found a significant increase in peroxisomal beta-oxidation, catalase activity, and peroxisomal number already after 3 days of dietary treatment. These changes were more pronounced after 3 weeks. Peroxisomal size was not changed. Positive correlations were found between peroxisomal enzyme activities and the number but not the size of the peroxisomes, and between catalase activity and beta-oxidation capacity. The mean peroxisomal diameter per animal was inversely proportional to catalase activity measured in homogenate. In myocardium, catalase activity was increased with duration of fish oil feeding. Peroxisomal staining, number, and size were also increased when compared to controls. In kidney, no alterations were observed.
Our results indicate a beneficial effect of a diet supplemented with fish oil on the peroxisomal metabolism in liver and myocardium; it differs from the changes induced by xenobiotic peroxisome proliferation.},
  author       = {De Craemer, Dirk and Vamecq, Joseph and Roels, Frank and Vall{\'e}e, Louis and Pauwels, Marina and Van den Branden, Christiane},
  issn         = {0022-2275},
  journal      = {JOURNAL OF LIPID RESEARCH},
  keyword      = {CATALASE CYTOCHEMISTRY,CATALASE ACTIVITY,MORPHOMETRY,DOCOSAHEXAENOIC ACID,POLYUNSATURATED FATTY ACIDS,PEROXISOMAL BETA-OXIDATION,MITOCHONDRIAL BETA-OXIDATION,RAT-LIVER,ACID OXIDATION,EICOSAPENTAENOIC ACID,HEPATOCELLULAR PEROXISOMES,HEPATIC PEROXISOMES,CLOFIBRIC ACID,ERUCIC-ACID,METABOLISM,ADRENOLEUKODYSTROPHY},
  language     = {eng},
  number       = {7},
  pages        = {1241--1250},
  title        = {Peroxisomes in liver, heart, and kidney of mice fed a commercial fish oil preparation: original data and review on peroxisomal changes induced by high-fat diets},
  url          = {http://www.jlr.org/cgi/content/abstract/35/7/1241},
  volume       = {35},
  year         = {1994},
}

Chicago
De Craemer, Dirk, Joseph Vamecq, Frank Roels, Louis Vallée, Marina Pauwels, and Christiane Van den Branden. 1994. “Peroxisomes in Liver, Heart, and Kidney of Mice Fed a Commercial Fish Oil Preparation: Original Data and Review on Peroxisomal Changes Induced by High-fat Diets.” Journal of Lipid Research 35 (7): 1241–1250.
APA
De Craemer, D., Vamecq, J., Roels, F., Vallée, L., Pauwels, M., & Van den Branden, C. (1994). Peroxisomes in liver, heart, and kidney of mice fed a commercial fish oil preparation: original data and review on peroxisomal changes induced by high-fat diets. JOURNAL OF LIPID RESEARCH, 35(7), 1241–1250.
Vancouver
1.
De Craemer D, Vamecq J, Roels F, Vallée L, Pauwels M, Van den Branden C. Peroxisomes in liver, heart, and kidney of mice fed a commercial fish oil preparation: original data and review on peroxisomal changes induced by high-fat diets. JOURNAL OF LIPID RESEARCH. 1994;35(7):1241–50.
MLA
De Craemer, Dirk, Joseph Vamecq, Frank Roels, et al. “Peroxisomes in Liver, Heart, and Kidney of Mice Fed a Commercial Fish Oil Preparation: Original Data and Review on Peroxisomal Changes Induced by High-fat Diets.” JOURNAL OF LIPID RESEARCH 35.7 (1994): 1241–1250. Print.