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Multilocus sequence analysis of Bosea species and description of Bosea lupini sp. nov., Bosea lathyri sp. nov. and Bosea robiniae sp. nov., isolated from legumes

Sofie De Meyer UGent and Anne Willems UGent (2012) INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 62(10). p.2505-2510
abstract
Gram-negative, rod-shaped bacteria were isolated from Lupinus polyphyllus, Lathyrus latifolius and Robinia pseudoacacia root nodules. Based on the 16S rRNA gene phylogeny, they were closely related to Bosea species (100 - 97%), belonging to the Alphaproteobacteria class and Bradyrhizobiaceae family. The closest relative for LMG 26383T, LMG 26379T and LMG 26381T were respectively, Bosea thiooxidans (99.6%), Bosea eneae (98.3%) and Bosea minatitlanensis (99.0%). Chemotaxonomic data, including the major fatty acid profiles supported the assignment of our strains to the genus Bosea. Analysis of the concatenated sequences of five housekeeping genes (atpD, dnaK, gyrB, recA and rpoB) and the results of DNA-DNA hybridisations, physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from each other and from the five validly published Bosea species. No nodA, nor nodC genes could be amplified, while nifH PCR gave non-specific products. On the basis of genotypic and phenotypic data three novel species, Bosea lupini sp. nov. (LMG 26383T = CCUG 61248 T), Bosea lathyri sp. nov. (= LMG 26379T= CCUG 61247 T) and Bosea robiniae sp. nov. (LMG 26381T= CCUG 61249 T) are proposed.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ENSIFER, GENES, BACTERIUM, HYBRIDIZATION, SINORHIZOBIUM, IDENTIFICATION, DEOXYRIBONUCLEIC-ACID, Bosea, nodule, rhizobia, MLSA, ARB, DNA
journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Int. J. Syst. Evol. Microbiol.
volume
62
issue
10
pages
2505 - 2510
Web of Science type
Article
Web of Science id
000311587800031
JCR category
MICROBIOLOGY
JCR impact factor
2.112 (2012)
JCR rank
68/116 (2012)
JCR quartile
3 (2012)
ISSN
1466-5026
DOI
10.1099/ijs.0.035477-0
project
FWO G.0058.07N
project
BOF 01J04807
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1985207
handle
http://hdl.handle.net/1854/LU-1985207
date created
2012-01-12 18:11:56
date last changed
2013-07-15 13:55:18
@article{1985207,
  abstract     = {Gram-negative, rod-shaped bacteria were isolated from Lupinus polyphyllus, Lathyrus latifolius and Robinia pseudoacacia root nodules. Based on the 16S rRNA gene phylogeny, they were closely related to Bosea species (100 - 97\%), belonging to the Alphaproteobacteria class and Bradyrhizobiaceae family. The closest relative for LMG 26383T, LMG 26379T and LMG 26381T were respectively, Bosea thiooxidans (99.6\%), Bosea eneae (98.3\%) and Bosea minatitlanensis (99.0\%). Chemotaxonomic data, including the major fatty acid profiles supported the assignment of our strains to the genus Bosea. Analysis of the concatenated sequences of five housekeeping genes (atpD, dnaK, gyrB, recA and rpoB) and the results of DNA-DNA hybridisations, physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from each other and from the five validly published Bosea species. No nodA, nor nodC genes could be amplified, while nifH PCR gave non-specific products. On the basis of genotypic and phenotypic data three novel species, Bosea lupini sp. nov. (LMG 26383T = CCUG 61248 T), Bosea lathyri sp. nov. (= LMG 26379T= CCUG 61247 T) and Bosea robiniae sp. nov. (LMG 26381T= CCUG 61249 T) are proposed.},
  author       = {De Meyer, Sofie and Willems, Anne},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {ENSIFER,GENES,BACTERIUM,HYBRIDIZATION,SINORHIZOBIUM,IDENTIFICATION,DEOXYRIBONUCLEIC-ACID,Bosea,nodule,rhizobia,MLSA,ARB,DNA},
  language     = {eng},
  number       = {10},
  pages        = {2505--2510},
  title        = {Multilocus sequence analysis of Bosea species and description of Bosea lupini sp. nov., Bosea lathyri sp. nov. and Bosea robiniae sp. nov., isolated from legumes},
  url          = {http://dx.doi.org/10.1099/ijs.0.035477-0},
  volume       = {62},
  year         = {2012},
}

Chicago
De Meyer, Sofie, and Anne Willems. 2012. “Multilocus Sequence Analysis of Bosea Species and Description of Bosea Lupini Sp. Nov., Bosea Lathyri Sp. Nov. and Bosea Robiniae Sp. Nov., Isolated from Legumes.” International Journal of Systematic and Evolutionary Microbiology 62 (10): 2505–2510.
APA
De Meyer, S., & Willems, A. (2012). Multilocus sequence analysis of Bosea species and description of Bosea lupini sp. nov., Bosea lathyri sp. nov. and Bosea robiniae sp. nov., isolated from legumes. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 62(10), 2505–2510.
Vancouver
1.
De Meyer S, Willems A. Multilocus sequence analysis of Bosea species and description of Bosea lupini sp. nov., Bosea lathyri sp. nov. and Bosea robiniae sp. nov., isolated from legumes. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2012;62(10):2505–10.
MLA
De Meyer, Sofie, and Anne Willems. “Multilocus Sequence Analysis of Bosea Species and Description of Bosea Lupini Sp. Nov., Bosea Lathyri Sp. Nov. and Bosea Robiniae Sp. Nov., Isolated from Legumes.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 62.10 (2012): 2505–2510. Print.