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Potential of Nepalese entomopathogenic nematodes as biocontrol agents against Holotrichia longipennis Blanch. (Coleoptera: Scarabaeidae)

Hari Bahadur Khatri-Chhetri, Govinda Prasad Timsina, Hira Kaji Manandhar and Maurice Moens UGent (2011) JOURNAL OF PEST SCIENCE. 84(4). p.457-469
abstract
Holotrichia longipennis Blanch. (Coleoptera: Scarabaeidae) is a serious pest of commercial crops in Siduwa, Dhankuta, Nepal. Seven indigenous isolates of entomopathogenic nematodes (Steinernema lamjungense LMT5, S. lamjungense SS4, S. everestense DKP4, S. abbasi CS1, S. sp. KL1, Heterorhabditis indica CK2 and H. indica CK6) were used in a series of bioassays against the insect. All isolates showed an increased dispersal in response to H. longipennis. Nematodes were more attracted towards third instar larvae than to second instars. Differences in penetration and multiplication in the insect were observed amongst the seven isolates. Steinernema lamjungense LMT5, S. everestense DKP4 and S. abbasi CS1 caused greater mortality than other isolates to different developmental stages. Pupae and second instar larvae were more susceptible than third instar larvae. Significant differences were observed in LT(50) values of the isolates against different stages of H. longipennis. Three isolates (S. lamjungense LMT5, S. everestense DKP4 and S. abbasi CS1) along with a commonly used insecticide (chlorpyrifos) were tested against this insect in pot and field experiments. In pot experiments using maize and cabbage as a host crop, S. lamjungense LMT5 and S. everestense DKP4 performed better than S. abbasi CS1 and yielded a mortality comparable with chlorpyrifos. Similar results were observed in field experiments 3 weeks after nematode application. These experiments overall suggest S. lamjungense LMT5 to be a promising biocontrol agent against H. longipennis followed by S. everestense DKP4 and S. abbasi CS1.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Dispersal, Entomopathogenic nematodes, Holotrichia longipennis, Nepal, Steinernema, Virulence, POPILLIA-JAPONICA COLEOPTERA, HETERORHABDITIS-BACTERIOPHORA, ENTOMOGENOUS NEMATODE, BIOLOGICAL-CONTROL, RHABDITIDA STEINERNEMATIDAE, HOST RECOGNITION, CARBON-DIOXIDE, BEETLE LARVAE, CARPOCAPSAE, SUSCEPTIBILITY, Heterorhabditis
journal title
JOURNAL OF PEST SCIENCE
J. Pest Sci.
volume
84
issue
4
pages
457 - 469
Web of Science type
Article
Web of Science id
000297136500007
JCR category
ENTOMOLOGY
JCR impact factor
1.509 (2011)
JCR rank
27/85 (2011)
JCR quartile
2 (2011)
ISSN
1612-4758
DOI
10.1007/s10340-011-0370-5
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1984544
handle
http://hdl.handle.net/1854/LU-1984544
date created
2012-01-12 13:08:18
date last changed
2016-12-19 15:45:27
@article{1984544,
  abstract     = {Holotrichia longipennis Blanch. (Coleoptera: Scarabaeidae) is a serious pest of commercial crops in Siduwa, Dhankuta, Nepal. Seven indigenous isolates of entomopathogenic nematodes (Steinernema lamjungense LMT5, S. lamjungense SS4, S. everestense DKP4, S. abbasi CS1, S. sp. KL1, Heterorhabditis indica CK2 and H. indica CK6) were used in a series of bioassays against the insect. All isolates showed an increased dispersal in response to H. longipennis. Nematodes were more attracted towards third instar larvae than to second instars. Differences in penetration and multiplication in the insect were observed amongst the seven isolates. Steinernema lamjungense LMT5, S. everestense DKP4 and S. abbasi CS1 caused greater mortality than other isolates to different developmental stages. Pupae and second instar larvae were more susceptible than third instar larvae. Significant differences were observed in LT(50) values of the isolates against different stages of H. longipennis. Three isolates (S. lamjungense LMT5, S. everestense DKP4 and S. abbasi CS1) along with a commonly used insecticide (chlorpyrifos) were tested against this insect in pot and field experiments. In pot experiments using maize and cabbage as a host crop, S. lamjungense LMT5 and S. everestense DKP4 performed better than S. abbasi CS1 and yielded a mortality comparable with chlorpyrifos. Similar results were observed in field experiments 3 weeks after nematode application. These experiments overall suggest S. lamjungense LMT5 to be a promising biocontrol agent against H. longipennis followed by S. everestense DKP4 and S. abbasi CS1.},
  author       = {Khatri-Chhetri, Hari Bahadur and Timsina, Govinda Prasad and Manandhar, Hira Kaji and Moens, Maurice},
  issn         = {1612-4758},
  journal      = {JOURNAL OF PEST SCIENCE},
  keyword      = {Dispersal,Entomopathogenic nematodes,Holotrichia longipennis,Nepal,Steinernema,Virulence,POPILLIA-JAPONICA COLEOPTERA,HETERORHABDITIS-BACTERIOPHORA,ENTOMOGENOUS NEMATODE,BIOLOGICAL-CONTROL,RHABDITIDA STEINERNEMATIDAE,HOST RECOGNITION,CARBON-DIOXIDE,BEETLE LARVAE,CARPOCAPSAE,SUSCEPTIBILITY,Heterorhabditis},
  language     = {eng},
  number       = {4},
  pages        = {457--469},
  title        = {Potential of Nepalese entomopathogenic nematodes as biocontrol agents against Holotrichia longipennis Blanch. (Coleoptera: Scarabaeidae)},
  url          = {http://dx.doi.org/10.1007/s10340-011-0370-5},
  volume       = {84},
  year         = {2011},
}

Chicago
Khatri-Chhetri, Hari Bahadur, Govinda Prasad Timsina, Hira Kaji Manandhar, and Maurice Moens. 2011. “Potential of Nepalese Entomopathogenic Nematodes as Biocontrol Agents Against Holotrichia Longipennis Blanch. (Coleoptera: Scarabaeidae).” Journal of Pest Science 84 (4): 457–469.
APA
Khatri-Chhetri, H. B., Timsina, G. P., Manandhar, H. K., & Moens, M. (2011). Potential of Nepalese entomopathogenic nematodes as biocontrol agents against Holotrichia longipennis Blanch. (Coleoptera: Scarabaeidae). JOURNAL OF PEST SCIENCE, 84(4), 457–469.
Vancouver
1.
Khatri-Chhetri HB, Timsina GP, Manandhar HK, Moens M. Potential of Nepalese entomopathogenic nematodes as biocontrol agents against Holotrichia longipennis Blanch. (Coleoptera: Scarabaeidae). JOURNAL OF PEST SCIENCE. 2011;84(4):457–69.
MLA
Khatri-Chhetri, Hari Bahadur, Govinda Prasad Timsina, Hira Kaji Manandhar, et al. “Potential of Nepalese Entomopathogenic Nematodes as Biocontrol Agents Against Holotrichia Longipennis Blanch. (Coleoptera: Scarabaeidae).” JOURNAL OF PEST SCIENCE 84.4 (2011): 457–469. Print.