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Functional analysis of genes involved in cell wall biosynthesis of the model species Brachypodium distachyon to improve saccharification

Steven Van Hulle UGent, Isabel Roldàn-Ruiz UGent, Erik Van Bockstaele and Hilde Muylle UGent (2010) Sustainable use of genetic diversity in forage and turf breeding. p.479-482
abstract
Members of the grass family are important as resource for the production of first generation bio-ethanol, which is based on the fermentation of starch and sucrose which is obtained from food crops like corn (US), wheat (Europe) or sugarcane (Brazil). If Europe wants to meet the objective of the new directive on the promotion of the use of energy from renewable sources, it will be necessary to make the transition from first to second generation conversion technologies for the production of bio-ethanol. These conversion techniques are based on the use of recalcitrant lignocellulosic biomass as feedstock. The energy contained in lignocellulosic biomass is largely entrapped in the plant cell wall, which is built up of cellulose, hemicellulose and lignin and can make up to 70% of the total plant biomass. To be able to produce ethanol from these rigid cell walls, the cellulose and hemicellulose need to be degraded first into monosaccharides. For the moment, this degradation constitutes a bottleneck in the process. Especially lignin is a disturbing factor. Therefore, an interesting approach to improve lignocellulosic crops is to reduce their lignin content. In this study, we use Brachypodium distachyon as a model to study the effect of up- or down regulation of genes with a key-role in the monolignol biosynthesis pathway on the saccharification efficiency. The general strategy and preliminary results of this study will be discussed.
Please use this url to cite or link to this publication:
author
organization
year
type
conference (proceedingsPaper)
publication status
published
subject
keyword
Functional analysis, Bio-ethanol, Lignin, LIGNIN BIOSYNTHESIS, AGROBACTERIUM-MEDIATED TRANSFORMATION, Brachypodium distachyon, EXPRESSION
in
Sustainable use of genetic diversity in forage and turf breeding
editor
Christian Huyghe
pages
479 - 482
publisher
Springer
place of publication
Berlin, Germany
conference name
Conference of the Eucarpia Fodder and Amenity Species Section
conference location
La Rochelle, France
conference start
2009-05-10
conference end
2009-05-14
Web of Science type
Proceedings Paper
Web of Science id
000280198100071
ISBN
9789048187058
DOI
10.1007/978-90-481-8706-5_71
language
English
UGent publication?
no
classification
P1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1984532
handle
http://hdl.handle.net/1854/LU-1984532
date created
2012-01-12 12:44:08
date last changed
2017-01-02 09:53:24
@inproceedings{1984532,
  abstract     = {Members of the grass family are important as resource for the production of first generation bio-ethanol, which is based on the fermentation of starch and sucrose which is obtained from food crops like corn (US), wheat (Europe) or sugarcane (Brazil). If Europe wants to meet the objective of the new directive on the promotion of the use of energy from renewable sources, it will be necessary to make the transition from first to second generation conversion technologies for the production of bio-ethanol. These conversion techniques are based on the use of recalcitrant lignocellulosic biomass as feedstock. The energy contained in lignocellulosic biomass is largely entrapped in the plant cell wall, which is built up of cellulose, hemicellulose and lignin and can make up to 70\% of the total plant biomass. To be able to produce ethanol from these rigid cell walls, the cellulose and hemicellulose need to be degraded first into monosaccharides. For the moment, this degradation constitutes a bottleneck in the process. Especially lignin is a disturbing factor. Therefore, an interesting approach to improve lignocellulosic crops is to reduce their lignin content. In this study, we use Brachypodium distachyon as a model to study the effect of up- or down regulation of genes with a key-role in the monolignol biosynthesis pathway on the saccharification efficiency. The general strategy and preliminary results of this study will be discussed.},
  author       = {Van Hulle, Steven and Rold{\`a}n-Ruiz, Isabel and Van Bockstaele, Erik and Muylle, Hilde},
  booktitle    = {Sustainable use of genetic diversity in forage and turf breeding},
  editor       = {Huyghe, Christian},
  isbn         = {9789048187058},
  keyword      = {Functional analysis,Bio-ethanol,Lignin,LIGNIN BIOSYNTHESIS,AGROBACTERIUM-MEDIATED TRANSFORMATION,Brachypodium distachyon,EXPRESSION},
  language     = {eng},
  location     = {La Rochelle, France},
  pages        = {479--482},
  publisher    = {Springer},
  title        = {Functional analysis of genes involved in cell wall biosynthesis of the model species Brachypodium distachyon to improve saccharification},
  url          = {http://dx.doi.org/10.1007/978-90-481-8706-5\_71},
  year         = {2010},
}

Chicago
Van Hulle, Steven, Isabel Roldàn-Ruiz, Erik Van Bockstaele, and Hilde Muylle. 2010. “Functional Analysis of Genes Involved in Cell Wall Biosynthesis of the Model Species Brachypodium Distachyon to Improve Saccharification.” In Sustainable Use of Genetic Diversity in Forage and Turf Breeding, ed. Christian Huyghe, 479–482. Berlin, Germany: Springer.
APA
Van Hulle, Steven, Roldàn-Ruiz, I., Van Bockstaele, E., & Muylle, H. (2010). Functional analysis of genes involved in cell wall biosynthesis of the model species Brachypodium distachyon to improve saccharification. In Christian Huyghe (Ed.), Sustainable use of genetic diversity in forage and turf breeding (pp. 479–482). Presented at the Conference of the Eucarpia Fodder and Amenity Species Section, Berlin, Germany: Springer.
Vancouver
1.
Van Hulle S, Roldàn-Ruiz I, Van Bockstaele E, Muylle H. Functional analysis of genes involved in cell wall biosynthesis of the model species Brachypodium distachyon to improve saccharification. In: Huyghe C, editor. Sustainable use of genetic diversity in forage and turf breeding. Berlin, Germany: Springer; 2010. p. 479–82.
MLA
Van Hulle, Steven, Isabel Roldàn-Ruiz, Erik Van Bockstaele, et al. “Functional Analysis of Genes Involved in Cell Wall Biosynthesis of the Model Species Brachypodium Distachyon to Improve Saccharification.” Sustainable Use of Genetic Diversity in Forage and Turf Breeding. Ed. Christian Huyghe. Berlin, Germany: Springer, 2010. 479–482. Print.