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CD27-deficient mice show normal NK-cell differentiation but impaired function upon stimulation

Veerle De Colvenaer, Sylvie Taveirne UGent, Maarten Delforche, Magda De Smedt, Bart Vandekerckhove UGent, Tom Taghon UGent, Louis Boon, Jean Plum and Georges Leclercq UGent (2011) IMMUNOLOGY AND CELL BIOLOGY. 89(7). p.803-811
abstract
Natural killer (NK) cells are part of the first line defense against tumors, parasites and virus-infected cells. Therefore, factors that control NK-cell numbers and their function are important. CD27 is constitutively expressed on NK cells and its expression correlates with sequential phases in NK-cell development, discriminating phenotypically and functionally different subsets within the NK-cell population. Although CD27 has been described to have an important regulatory role in effector and memory T and B lymphocytes, its role in NK-cell biology remains to be addressed. In this study, we used CD27(-/-) mice to investigate the role of CD27 in NK-cell development and function, both during the resting state and upon stimulation. The results show that NK-cell numbers are not impaired in CD27(-/-) mice. Moreover, CD27(-/-) NK cells reach full phenotypic maturity, evidenced by normal expression of CD49b, CD43 and CD11b. Expression of activating receptors is unaltered, whereas expression of several inhibitory receptors is increased. Cytotoxicity and interferon-gamma production by NK cells from CD27(-/-) mice in the resting state are normal. However, upon in vivo anti-CD40- or poly-I: C-mediated activation, or in vitro interleukin-15 priming plus anti-NKp46 stimulation, the absence of CD27 results in decreased cytolytic activity and cytokine production by spleen and liver NK cells. In conclusion, this study demonstrates that CD27 is dispensable for the development of functional NK cells. However, upon stimulation of NK cells, CD27 displays an important role in their activation and functionality.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
cytotoxicity, cell differentiation and activation, natural killer cells, NATURAL-KILLER-CELLS, MURINE BONE-MARROW, CD4(+) T-CELLS, KAPPA-B ACTIVATION, DENDRITIC CELLS, IN-VIVO, TNF RECEPTOR, LIGAND CD70, CD27, PROGENITORS
journal title
IMMUNOLOGY AND CELL BIOLOGY
Immunol. Cell Biol.
volume
89
issue
7
pages
803 - 811
Web of Science type
Article
Web of Science id
000295791500113
JCR category
IMMUNOLOGY
JCR impact factor
3.661 (2011)
JCR rank
45/138 (2011)
JCR quartile
2 (2011)
ISSN
0818-9641
DOI
10.1038/icb.2010.171
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1984207
handle
http://hdl.handle.net/1854/LU-1984207
date created
2012-01-12 10:14:22
date last changed
2016-12-19 15:42:53
@article{1984207,
  abstract     = {Natural killer (NK) cells are part of the first line defense against tumors, parasites and virus-infected cells. Therefore, factors that control NK-cell numbers and their function are important. CD27 is constitutively expressed on NK cells and its expression correlates with sequential phases in NK-cell development, discriminating phenotypically and functionally different subsets within the NK-cell population. Although CD27 has been described to have an important regulatory role in effector and memory T and B lymphocytes, its role in NK-cell biology remains to be addressed. In this study, we used CD27(-/-) mice to investigate the role of CD27 in NK-cell development and function, both during the resting state and upon stimulation. The results show that NK-cell numbers are not impaired in CD27(-/-) mice. Moreover, CD27(-/-) NK cells reach full phenotypic maturity, evidenced by normal expression of CD49b, CD43 and CD11b. Expression of activating receptors is unaltered, whereas expression of several inhibitory receptors is increased. Cytotoxicity and interferon-gamma production by NK cells from CD27(-/-) mice in the resting state are normal. However, upon in vivo anti-CD40- or poly-I: C-mediated activation, or in vitro interleukin-15 priming plus anti-NKp46 stimulation, the absence of CD27 results in decreased cytolytic activity and cytokine production by spleen and liver NK cells. In conclusion, this study demonstrates that CD27 is dispensable for the development of functional NK cells. However, upon stimulation of NK cells, CD27 displays an important role in their activation and functionality.},
  author       = {De Colvenaer, Veerle and Taveirne, Sylvie and Delforche, Maarten and De Smedt, Magda and Vandekerckhove, Bart and Taghon, Tom and Boon, Louis and Plum, Jean and Leclercq, Georges},
  issn         = {0818-9641},
  journal      = {IMMUNOLOGY AND CELL BIOLOGY},
  keyword      = {cytotoxicity,cell differentiation and activation,natural killer cells,NATURAL-KILLER-CELLS,MURINE BONE-MARROW,CD4(+) T-CELLS,KAPPA-B ACTIVATION,DENDRITIC CELLS,IN-VIVO,TNF RECEPTOR,LIGAND CD70,CD27,PROGENITORS},
  language     = {eng},
  number       = {7},
  pages        = {803--811},
  title        = {CD27-deficient mice show normal NK-cell differentiation but impaired function upon stimulation},
  url          = {http://dx.doi.org/10.1038/icb.2010.171},
  volume       = {89},
  year         = {2011},
}

Chicago
De Colvenaer, Veerle, Sylvie Taveirne, Maarten Delforche, Magda De Smedt, Bart Vandekerckhove, Tom Taghon, Louis Boon, Jean Plum, and Georges Leclercq. 2011. “CD27-deficient Mice Show Normal NK-cell Differentiation but Impaired Function Upon Stimulation.” Immunology and Cell Biology 89 (7): 803–811.
APA
De Colvenaer, V., Taveirne, S., Delforche, M., De Smedt, M., Vandekerckhove, B., Taghon, T., Boon, L., et al. (2011). CD27-deficient mice show normal NK-cell differentiation but impaired function upon stimulation. IMMUNOLOGY AND CELL BIOLOGY, 89(7), 803–811.
Vancouver
1.
De Colvenaer V, Taveirne S, Delforche M, De Smedt M, Vandekerckhove B, Taghon T, et al. CD27-deficient mice show normal NK-cell differentiation but impaired function upon stimulation. IMMUNOLOGY AND CELL BIOLOGY. 2011;89(7):803–11.
MLA
De Colvenaer, Veerle, Sylvie Taveirne, Maarten Delforche, et al. “CD27-deficient Mice Show Normal NK-cell Differentiation but Impaired Function Upon Stimulation.” IMMUNOLOGY AND CELL BIOLOGY 89.7 (2011): 803–811. Print.