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Langerhans cells are not required for epidermal Vγ3 T cell homeostasis and function

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Abstract
This study tested the hypothesis that V gamma 3 TCR-bearing T cells are influenced by LCs. V gamma 3 T cells and LCs are located in the epidermis of mice. V gamma 3 T cells represent the main T cell population in the skin epithelium and play a crucial role in maintaining the skin integrity, whereas LCs are professional APCs. Although V gamma 3 T cells and LCs form an interdigitating network in the epidermis, not much is known about their reciprocal influence and/or interdependence. We used two different LC-deficient mouse models, in which LCs are constitutively or inducibly depleted, to investigate the role of LCs in maturation, homeostasis, and function of V gamma 3 T cells. We show that V gamma 3 T cell numbers are unaltered by LC deficiency, and V gamma 3 T cells isolated from LC-deficient mice are phenotypically and upon in vitro stimulation, functionally indistinguishable from V gamma 3 T cells isolated from WT mice based on their cytotoxic potential and cytokine production. Additionally, in vivo skin-wounding experiments show no major difference in response of V gamma 3 T cells to wounding in the absence or presence of LCs. These observations indicate that V gamma 3 T cells develop and function independently of LCs
Keywords
LC deficiency, Langerin DTR mice, NATURAL-KILLER-CELLS, DENDRITIC CELLS, CONTACT HYPERSENSITIVITY, DEFICIENT MICE, BONE-MARROW, GAMMA-GENES, IN-VITRO, RECEPTOR, SKIN, EXPRESSION, Langerin DTA mice, skin

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Chicago
Taveirne, Sylvie, Veerle De Colvenaer, Tina Van Den Broeck, Els Van Ammel, Clare L Bennett, Tom Taghon, Bart Vandekerckhove, et al. 2011. “Langerhans Cells Are Not Required for Epidermal Vγ3 T Cell Homeostasis and Function.” Journal of Leukocyte Biology 90 (1): 61–68.
APA
Taveirne, S., De Colvenaer, V., Van Den Broeck, T., Van Ammel, E., Bennett, C. L., Taghon, T., Vandekerckhove, B., et al. (2011). Langerhans cells are not required for epidermal Vγ3 T cell homeostasis and function. JOURNAL OF LEUKOCYTE BIOLOGY, 90(1), 61–68.
Vancouver
1.
Taveirne S, De Colvenaer V, Van Den Broeck T, Van Ammel E, Bennett CL, Taghon T, et al. Langerhans cells are not required for epidermal Vγ3 T cell homeostasis and function. JOURNAL OF LEUKOCYTE BIOLOGY. 2011;90(1):61–8.
MLA
Taveirne, Sylvie, Veerle De Colvenaer, Tina Van Den Broeck, et al. “Langerhans Cells Are Not Required for Epidermal Vγ3 T Cell Homeostasis and Function.” JOURNAL OF LEUKOCYTE BIOLOGY 90.1 (2011): 61–68. Print.
@article{1984194,
  abstract     = {This study tested the hypothesis that V gamma 3 TCR-bearing T cells are influenced by LCs. V gamma 3 T cells and LCs are located in the epidermis of mice. V gamma 3 T cells represent the main T cell population in the skin epithelium and play a crucial role in maintaining the skin integrity, whereas LCs are professional APCs. Although V gamma 3 T cells and LCs form an interdigitating network in the epidermis, not much is known about their reciprocal influence and/or interdependence. We used two different LC-deficient mouse models, in which LCs are constitutively or inducibly depleted, to investigate the role of LCs in maturation, homeostasis, and function of V gamma 3 T cells. We show that V gamma 3 T cell numbers are unaltered by LC deficiency, and V gamma 3 T cells isolated from LC-deficient mice are phenotypically and upon in vitro stimulation, functionally indistinguishable from V gamma 3 T cells isolated from WT mice based on their cytotoxic potential and cytokine production. Additionally, in vivo skin-wounding experiments show no major difference in response of V gamma 3 T cells to wounding in the absence or presence of LCs. These observations indicate that V gamma 3 T cells develop and function independently of LCs},
  author       = {Taveirne, Sylvie and De Colvenaer, Veerle and Van Den Broeck, Tina and Van Ammel, Els and Bennett, Clare L and Taghon, Tom and Vandekerckhove, Bart and Plum, Jean and Clausen, Bj{\"o}rn E and Kaplan, Daniel H and Leclercq, Georges},
  issn         = {0741-5400},
  journal      = {JOURNAL OF LEUKOCYTE BIOLOGY},
  keyword      = {LC deficiency,Langerin DTR mice,NATURAL-KILLER-CELLS,DENDRITIC CELLS,CONTACT HYPERSENSITIVITY,DEFICIENT MICE,BONE-MARROW,GAMMA-GENES,IN-VITRO,RECEPTOR,SKIN,EXPRESSION,Langerin DTA mice,skin},
  language     = {eng},
  number       = {1},
  pages        = {61--68},
  title        = {Langerhans cells are not required for epidermal V\ensuremath{\gamma}3 T cell homeostasis and function},
  url          = {http://dx.doi.org/10.1189/jlb.1010581},
  volume       = {90},
  year         = {2011},
}

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