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A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa

SARAH GLORIEUX UGent, Herman Favoreel UGent, Lennert Steukers UGent, Annelies Vandekerckhove UGent and Hans Nauwynck UGent (2011) VETERINARY RESEARCH. 42.
abstract
Several alphaherpesviruses breach the basement membrane during mucosal invasion. In the present study, the role of proteases in this process was examined. The serine protease-specific inhibitor AEBSF inhibited penetration of the basement membrane by the porcine alphaherpesvirus pseudorabies virus (PRV) by 88.1% without affecting lateral spread. Inhibitors of aspartic-, cysteine-, and metalloproteases did not inhibit viral penetration of the basement membrane. Further analysis using the Soybean Type I-S trypsin inhibitor for the serine protease subcategory of trypsin-like serine proteases resulted in a 96.9% reduction in plaque depth underneath the basement membrane. These data reveal a role of a trypsin-like serine protease in PRV penetration of the basement membrane.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
HEALTH, PIGLETS, PEPTIDASES, PIGS, PATHOGENESIS, EXPRESSION, VIRULENT VIRUS, NERVOUS PATHWAY, INTRANASAL INFECTION, AUJESZKYS-DISEASE VIRUS
journal title
VETERINARY RESEARCH
Vet. Res.
volume
42
article_number
58
pages
5 pages
Web of Science type
Article
Web of Science id
000290663200002
JCR category
VETERINARY SCIENCES
JCR impact factor
4.06 (2011)
JCR rank
1/141 (2011)
JCR quartile
1 (2011)
ISSN
0928-4249
DOI
10.1186/1297-9716-42-58
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1983166
handle
http://hdl.handle.net/1854/LU-1983166
date created
2012-01-11 11:30:51
date last changed
2012-01-16 11:34:36
@article{1983166,
  abstract     = {Several alphaherpesviruses breach the basement membrane during mucosal invasion. In the present study, the role of proteases in this process was examined. The serine protease-specific inhibitor AEBSF inhibited penetration of the basement membrane by the porcine alphaherpesvirus pseudorabies virus (PRV) by 88.1\% without affecting lateral spread. Inhibitors of aspartic-, cysteine-, and metalloproteases did not inhibit viral penetration of the basement membrane. Further analysis using the Soybean Type I-S trypsin inhibitor for the serine protease subcategory of trypsin-like serine proteases resulted in a 96.9\% reduction in plaque depth underneath the basement membrane. These data reveal a role of a trypsin-like serine protease in PRV penetration of the basement membrane.},
  articleno    = {58},
  author       = {GLORIEUX, SARAH and Favoreel, Herman and Steukers, Lennert and Vandekerckhove, Annelies and Nauwynck, Hans},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {HEALTH,PIGLETS,PEPTIDASES,PIGS,PATHOGENESIS,EXPRESSION,VIRULENT VIRUS,NERVOUS PATHWAY,INTRANASAL INFECTION,AUJESZKYS-DISEASE VIRUS},
  language     = {eng},
  pages        = {5},
  title        = {A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa},
  url          = {http://dx.doi.org/10.1186/1297-9716-42-58},
  volume       = {42},
  year         = {2011},
}

Chicago
Glorieux, Sarah, Herman Favoreel, Lennert Steukers, Annelies Vandekerckhove, and Hans Nauwynck. 2011. “A Trypsin-like Serine Protease Is Involved in Pseudorabies Virus Invasion Through the Basement Membrane Barrier of Porcine Nasal Respiratory Mucosa.” Veterinary Research 42.
APA
Glorieux, S., Favoreel, H., Steukers, L., Vandekerckhove, A., & Nauwynck, H. (2011). A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa. VETERINARY RESEARCH, 42.
Vancouver
1.
Glorieux S, Favoreel H, Steukers L, Vandekerckhove A, Nauwynck H. A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa. VETERINARY RESEARCH. 2011;42.
MLA
Glorieux, Sarah, Herman Favoreel, Lennert Steukers, et al. “A Trypsin-like Serine Protease Is Involved in Pseudorabies Virus Invasion Through the Basement Membrane Barrier of Porcine Nasal Respiratory Mucosa.” VETERINARY RESEARCH 42 (2011): n. pag. Print.