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A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa

Sarah Glorieux (UGent) , Herman Favoreel (UGent) , Lennert Steukers (UGent) , Annelies Vandekerckhove (UGent) and Hans Nauwynck (UGent)
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Abstract
Several alphaherpesviruses breach the basement membrane during mucosal invasion. In the present study, the role of proteases in this process was examined. The serine protease-specific inhibitor AEBSF inhibited penetration of the basement membrane by the porcine alphaherpesvirus pseudorabies virus (PRV) by 88.1% without affecting lateral spread. Inhibitors of aspartic-, cysteine-, and metalloproteases did not inhibit viral penetration of the basement membrane. Further analysis using the Soybean Type I-S trypsin inhibitor for the serine protease subcategory of trypsin-like serine proteases resulted in a 96.9% reduction in plaque depth underneath the basement membrane. These data reveal a role of a trypsin-like serine protease in PRV penetration of the basement membrane.
Keywords
HEALTH, PIGLETS, PEPTIDASES, PIGS, PATHOGENESIS, EXPRESSION, VIRULENT VIRUS, NERVOUS PATHWAY, INTRANASAL INFECTION, AUJESZKYS-DISEASE VIRUS

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Chicago
Glorieux, Sarah, Herman Favoreel, Lennert Steukers, Annelies Vandekerckhove, and Hans Nauwynck. 2011. “A Trypsin-like Serine Protease Is Involved in Pseudorabies Virus Invasion Through the Basement Membrane Barrier of Porcine Nasal Respiratory Mucosa.” Veterinary Research 42.
APA
Glorieux, S., Favoreel, H., Steukers, L., Vandekerckhove, A., & Nauwynck, H. (2011). A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa. VETERINARY RESEARCH, 42.
Vancouver
1.
Glorieux S, Favoreel H, Steukers L, Vandekerckhove A, Nauwynck H. A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa. VETERINARY RESEARCH. 2011;42.
MLA
Glorieux, Sarah, Herman Favoreel, Lennert Steukers, et al. “A Trypsin-like Serine Protease Is Involved in Pseudorabies Virus Invasion Through the Basement Membrane Barrier of Porcine Nasal Respiratory Mucosa.” VETERINARY RESEARCH 42 (2011): n. pag. Print.
@article{1983166,
  abstract     = {Several alphaherpesviruses breach the basement membrane during mucosal invasion. In the present study, the role of proteases in this process was examined. The serine protease-specific inhibitor AEBSF inhibited penetration of the basement membrane by the porcine alphaherpesvirus pseudorabies virus (PRV) by 88.1\% without affecting lateral spread. Inhibitors of aspartic-, cysteine-, and metalloproteases did not inhibit viral penetration of the basement membrane. Further analysis using the Soybean Type I-S trypsin inhibitor for the serine protease subcategory of trypsin-like serine proteases resulted in a 96.9\% reduction in plaque depth underneath the basement membrane. These data reveal a role of a trypsin-like serine protease in PRV penetration of the basement membrane.},
  articleno    = {58},
  author       = {Glorieux, Sarah and Favoreel, Herman and Steukers, Lennert and Vandekerckhove, Annelies and Nauwynck, Hans},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {HEALTH,PIGLETS,PEPTIDASES,PIGS,PATHOGENESIS,EXPRESSION,VIRULENT VIRUS,NERVOUS PATHWAY,INTRANASAL INFECTION,AUJESZKYS-DISEASE VIRUS},
  language     = {eng},
  pages        = {5},
  title        = {A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa},
  url          = {http://dx.doi.org/10.1186/1297-9716-42-58},
  volume       = {42},
  year         = {2011},
}

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