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Infection with the gastrointestinal nematode Ostertagia ostertagi in cattle affects mucus biosynthesis in the abomasum

Manuela Rinaldi UGent, Leentje Dreesen, Prisca Hoorens, Robert W Li, Edwin Claerebout UGent, Bruno Goddeeris, Jozef Vercruysse UGent, Wim Van Den Broeck UGent and Peter Geldhof UGent (2011) VETERINARY RESEARCH. 42.
abstract
The mucus layer in the gastrointestinal (GI) tract is considered to be the first line of defense to the external environment. Alteration in mucus components has been reported to occur during intestinal nematode infection in ruminants, but the role of mucus in response to abomasal parasites remains largely unclear. The aim of the current study was to analyze the effects of an Ostertagia ostertagi infection on the abomasal mucus biosynthesis in cattle. Increased gene expression of MUC1, MUC6 and MUC20 was observed, while MUC5AC did not change during infection. Qualitative changes of mucins, related to sugar composition, were also observed. AB-PAS and HID-AB stainings highlighted a decrease in neutral and an increase in acidic mucins, throughout the infection. Several genes involved in mucin core structure synthesis, branching and oligomerization, such as GCNT3, GCNT4, A4GNT and protein disulphide isomerases were found to be upregulated. Increase in mucin fucosylation was observed using the lectin UEA-I and through the evaluation of fucosyltransferases gene expression levels. Finally, transcription levels of 2 trefoil factors, TFF1 and TFF3, which are co-expressed with mucins in the GI tract, were also found to be significantly upregulated in infected animals. Although the alterations in mucus biosynthesis started early during infection, the biggest effects were found when adult worms were present on the surface of the abomasal mucosa and are likely caused by the alterations in mucosal cell populations, characterized by hyperplasia of mucus secreting cells.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DISULFIDE-ISOMERASE FAMILY, MUCOSAL BARRIER, HELICOBACTER-PYLORI INFECTION, NIPPOSTRONGYLUS-BRASILIENSIS, TREFOIL FACTOR, HAEMONCHUS-CONTORTUS, GASTRIC MUCIN, TELADORSAGIA-CIRCUMCINCTA, GOBLET CELL, TFF-PEPTIDES
journal title
VETERINARY RESEARCH
Vet. Res.
volume
42
article number
61
pages
10 pages
Web of Science type
Article
Web of Science id
000291101300001
JCR category
VETERINARY SCIENCES
JCR impact factor
4.06 (2011)
JCR rank
1/141 (2011)
JCR quartile
1 (2011)
ISSN
0928-4249
DOI
10.1186/1297-9716-42-61
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1983109
handle
http://hdl.handle.net/1854/LU-1983109
date created
2012-01-11 11:18:03
date last changed
2017-02-16 15:36:03
@article{1983109,
  abstract     = {The mucus layer in the gastrointestinal (GI) tract is considered to be the first line of defense to the external environment. Alteration in mucus components has been reported to occur during intestinal nematode infection in ruminants, but the role of mucus in response to abomasal parasites remains largely unclear. The aim of the current study was to analyze the effects of an Ostertagia ostertagi infection on the abomasal mucus biosynthesis in cattle. Increased gene expression of MUC1, MUC6 and MUC20 was observed, while MUC5AC did not change during infection. Qualitative changes of mucins, related to sugar composition, were also observed. AB-PAS and HID-AB stainings highlighted a decrease in neutral and an increase in acidic mucins, throughout the infection. Several genes involved in mucin core structure synthesis, branching and oligomerization, such as GCNT3, GCNT4, A4GNT and protein disulphide isomerases were found to be upregulated. Increase in mucin fucosylation was observed using the lectin UEA-I and through the evaluation of fucosyltransferases gene expression levels. Finally, transcription levels of 2 trefoil factors, TFF1 and TFF3, which are co-expressed with mucins in the GI tract, were also found to be significantly upregulated in infected animals. Although the alterations in mucus biosynthesis started early during infection, the biggest effects were found when adult worms were present on the surface of the abomasal mucosa and are likely caused by the alterations in mucosal cell populations, characterized by hyperplasia of mucus secreting cells.},
  articleno    = {61},
  author       = {Rinaldi, Manuela and Dreesen, Leentje and Hoorens, Prisca and Li, Robert W and Claerebout, Edwin and Goddeeris, Bruno and Vercruysse, Jozef and Van Den Broeck, Wim and Geldhof, Peter},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {DISULFIDE-ISOMERASE FAMILY,MUCOSAL BARRIER,HELICOBACTER-PYLORI INFECTION,NIPPOSTRONGYLUS-BRASILIENSIS,TREFOIL FACTOR,HAEMONCHUS-CONTORTUS,GASTRIC MUCIN,TELADORSAGIA-CIRCUMCINCTA,GOBLET CELL,TFF-PEPTIDES},
  language     = {eng},
  pages        = {10},
  title        = {Infection with the gastrointestinal nematode Ostertagia ostertagi in cattle affects mucus biosynthesis in the abomasum},
  url          = {http://dx.doi.org/10.1186/1297-9716-42-61},
  volume       = {42},
  year         = {2011},
}

Chicago
Rinaldi, Manuela, Leentje Dreesen, Prisca Hoorens, Robert W Li, Edwin Claerebout, Bruno Goddeeris, Jozef Vercruysse, Wim Van Den Broeck, and Peter Geldhof. 2011. “Infection with the Gastrointestinal Nematode Ostertagia Ostertagi in Cattle Affects Mucus Biosynthesis in the Abomasum.” Veterinary Research 42.
APA
Rinaldi, M., Dreesen, L., Hoorens, P., Li, R. W., Claerebout, E., Goddeeris, B., Vercruysse, J., et al. (2011). Infection with the gastrointestinal nematode Ostertagia ostertagi in cattle affects mucus biosynthesis in the abomasum. VETERINARY RESEARCH, 42.
Vancouver
1.
Rinaldi M, Dreesen L, Hoorens P, Li RW, Claerebout E, Goddeeris B, et al. Infection with the gastrointestinal nematode Ostertagia ostertagi in cattle affects mucus biosynthesis in the abomasum. VETERINARY RESEARCH. 2011;42.
MLA
Rinaldi, Manuela, Leentje Dreesen, Prisca Hoorens, et al. “Infection with the Gastrointestinal Nematode Ostertagia Ostertagi in Cattle Affects Mucus Biosynthesis in the Abomasum.” VETERINARY RESEARCH 42 (2011): n. pag. Print.