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A comparison of the sensitivity and fecal egg counts of the McMaster egg counting and Kato-Katz thick smear methods for soil-transmitted Helminths

Bruno Levecke UGent, Jerzy M Behnke, Sitara SR Ajjampur, Marco Albonico, Shaali M Ame, Johannes Charlier UGent, Stefan M Geiger, Nguyen TV Hoa, Romuald I Kamwa Ngassam and Andrew C Kotze, et al. (2011) PLOS NEGLECTED TROPICAL DISEASES. 5(6).
abstract
Background: The Kato-Katz thick smear (Kato-Katz) is the diagnostic method recommended for monitoring large-scale treatment programs implemented for the control of soil-transmitted helminths (STH) in public health, yet it is difficult to standardize. A promising alternative is the McMaster egg counting method (McMaster), commonly used in veterinary parasitology, but rarely so for the detection of STH in human stool. Methodology/Principal Findings: The Kato-Katz and McMaster methods were compared for the detection of STH in 1,543 subjects resident in five countries across Africa, Asia and South America. The consistency of the performance of both methods in different trials, the validity of the fixed multiplication factor employed in the Kato-Katz method and the accuracy of these methods for estimating 'true' drug efficacies were assessed. The Kato-Katz method detected significantly more Ascaris lumbricoides infections (88.1% vs. 75.6%, p < 0.001), whereas the difference in sensitivity between the two methods was nonsignificant for hookworm (78.3% vs. 72.4%) and Trichuris trichiura (82.6% vs. 80.3%). The sensitivity of the methods varied significantly across trials and magnitude of fecal egg counts (FEC). Quantitative comparison revealed a significant correlation (Rs > 0.32) in FEC between both methods, and indicated no significant difference in FEC, except for A. lumbricoides, where the Kato-Katz resulted in significantly higher FEC (14,197 eggs per gram of stool (EPG) vs. 5,982 EPG). For the Kato-Katz, the fixed multiplication factor resulted in significantly higher FEC than the multiplication factor adjusted for mass of feces examined for A. lumbricoides (16,538 EPG vs. 15,396 EPG) and T. trichiura (1,490 EPG vs. 1,363 EPG), but not for hookworm. The McMaster provided more accurate efficacy results (absolute difference to 'true' drug efficacy: 1.7% vs. 4.5%). Conclusions/Significance: The McMaster is an alternative method for monitoring large-scale treatment programs. It is a robust (accurate multiplication factor) and accurate (reliable efficacy results) method, which can be easily standardized.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
NEMATODES, RESISTANCE, FECES, ALBENDAZOLE, INFECTIONS, PUBLIC-HEALTH, SCHOOL-CHILDREN, TRICHURIS-TRICHIURA, MONITORING DRUG EFFICACY, ASCARIS-LUMBRICOIDES
journal title
PLOS NEGLECTED TROPICAL DISEASES
Plos Neglect. Trop. Dis.
volume
5
issue
6
article_number
e1201
pages
10 pages
Web of Science type
Article
Web of Science id
000292139600037
JCR category
TROPICAL MEDICINE
JCR impact factor
4.716 (2011)
JCR rank
1/20 (2011)
JCR quartile
1 (2011)
ISSN
1935-2727
DOI
10.1371/journal.pntd.0001201
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1983019
handle
http://hdl.handle.net/1854/LU-1983019
date created
2012-01-11 11:10:30
date last changed
2012-01-13 10:26:37
@article{1983019,
  abstract     = {Background: The Kato-Katz thick smear (Kato-Katz) is the diagnostic method recommended for monitoring large-scale treatment programs implemented for the control of soil-transmitted helminths (STH) in public health, yet it is difficult to standardize. A promising alternative is the McMaster egg counting method (McMaster), commonly used in veterinary parasitology, but rarely so for the detection of STH in human stool. 
Methodology/Principal Findings: The Kato-Katz and McMaster methods were compared for the detection of STH in 1,543 subjects resident in five countries across Africa, Asia and South America. The consistency of the performance of both methods in different trials, the validity of the fixed multiplication factor employed in the Kato-Katz method and the accuracy of these methods for estimating 'true' drug efficacies were assessed. The Kato-Katz method detected significantly more Ascaris lumbricoides infections (88.1\% vs. 75.6\%, p {\textlangle} 0.001), whereas the difference in sensitivity between the two methods was nonsignificant for hookworm (78.3\% vs. 72.4\%) and Trichuris trichiura (82.6\% vs. 80.3\%). The sensitivity of the methods varied significantly across trials and magnitude of fecal egg counts (FEC). Quantitative comparison revealed a significant correlation (Rs {\textrangle} 0.32) in FEC between both methods, and indicated no significant difference in FEC, except for A. lumbricoides, where the Kato-Katz resulted in significantly higher FEC (14,197 eggs per gram of stool (EPG) vs. 5,982 EPG). For the Kato-Katz, the fixed multiplication factor resulted in significantly higher FEC than the multiplication factor adjusted for mass of feces examined for A. lumbricoides (16,538 EPG vs. 15,396 EPG) and T. trichiura (1,490 EPG vs. 1,363 EPG), but not for hookworm. The McMaster provided more accurate efficacy results (absolute difference to 'true' drug efficacy: 1.7\% vs. 4.5\%). 
Conclusions/Significance: The McMaster is an alternative method for monitoring large-scale treatment programs. It is a robust (accurate multiplication factor) and accurate (reliable efficacy results) method, which can be easily standardized.},
  articleno    = {e1201},
  author       = {Levecke, Bruno and Behnke, Jerzy M and Ajjampur, Sitara SR and Albonico, Marco and Ame, Shaali M and Charlier, Johannes and Geiger, Stefan M and Hoa, Nguyen TV and Ngassam, Romuald I Kamwa and Kotze, Andrew C and McCarthy, James S and Montresor, Antonio and Periago, Maria V and Roy, Sheela and Tchuente, Louis-Albert Tchuem and Thach, DTC and Vercruysse, Jozef},
  issn         = {1935-2727},
  journal      = {PLOS NEGLECTED TROPICAL DISEASES},
  keyword      = {NEMATODES,RESISTANCE,FECES,ALBENDAZOLE,INFECTIONS,PUBLIC-HEALTH,SCHOOL-CHILDREN,TRICHURIS-TRICHIURA,MONITORING DRUG EFFICACY,ASCARIS-LUMBRICOIDES},
  language     = {eng},
  number       = {6},
  pages        = {10},
  title        = {A comparison of the sensitivity and fecal egg counts of the McMaster egg counting and Kato-Katz thick smear methods for soil-transmitted Helminths},
  url          = {http://dx.doi.org/10.1371/journal.pntd.0001201},
  volume       = {5},
  year         = {2011},
}

Chicago
Levecke, Bruno, Jerzy M Behnke, Sitara SR Ajjampur, Marco Albonico, Shaali M Ame, Johannes Charlier, Stefan M Geiger, et al. 2011. “A Comparison of the Sensitivity and Fecal Egg Counts of the McMaster Egg Counting and Kato-Katz Thick Smear Methods for Soil-transmitted Helminths.” Plos Neglected Tropical Diseases 5 (6).
APA
Levecke, B., Behnke, J. M., Ajjampur, S. S., Albonico, M., Ame, S. M., Charlier, J., Geiger, S. M., et al. (2011). A comparison of the sensitivity and fecal egg counts of the McMaster egg counting and Kato-Katz thick smear methods for soil-transmitted Helminths. PLOS NEGLECTED TROPICAL DISEASES, 5(6).
Vancouver
1.
Levecke B, Behnke JM, Ajjampur SS, Albonico M, Ame SM, Charlier J, et al. A comparison of the sensitivity and fecal egg counts of the McMaster egg counting and Kato-Katz thick smear methods for soil-transmitted Helminths. PLOS NEGLECTED TROPICAL DISEASES. 2011;5(6).
MLA
Levecke, Bruno, Jerzy M Behnke, Sitara SR Ajjampur, et al. “A Comparison of the Sensitivity and Fecal Egg Counts of the McMaster Egg Counting and Kato-Katz Thick Smear Methods for Soil-transmitted Helminths.” PLOS NEGLECTED TROPICAL DISEASES 5.6 (2011): n. pag. Print.