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Herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa

Sarah Glorieux, Claus Bachert UGent, Herman Favoreel UGent, Annelies Vandekerckhove UGent, Lennert Steukers, Anamaria Rekecki UGent, Wim Van Den Broeck UGent, Joline Goossens UGent, Siska Croubels UGent, Reginald F Clayton, et al. (2011) PLOS ONE. 6(7).
abstract
Background: Herpes simplex virus infections are highly prevalent in humans. However, the current therapeutics suffer important drawbacks such as limited results in neonates, increasing occurrence of resistance and impeded treatment of stromal infections. Remarkably, interactions of herpesviruses with human mucosa, the locus of infection, remain poorly understood and the underlying mechanisms in stromal infection remain controversial. Methodology/Principal Findings: A human model consisting of nasal respiratory mucosa explants was characterised. Viability and integrity were examined during 96 h of cultivation. HSV1-mucosa interactions were analysed. In particular, we investigated whether HSV1 is able to reach the stroma. Explant viability and integrity remained preserved. HSV1 induced rounding up and loosening of epithelial cells with very few apoptotic and necrotic cells observed. Following 16-24 h of infection, HSV1 penetrated the basement membrane and replicated in the underlying lamina propria. Conclusions/Significance: This human explant model can be used to study virus-mucosa interactions and viral mucosal invasion mechanisms. Using this model, our results provide a novel insight into the HSV1 stromal invasion mechanism and for the first time directly demonstrate that HSV1 can penetrate the basement membrane.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
IN-VITRO, BLOCKS APOPTOSIS, FLOW-CYTOMETRY, US3 PROTEIN-KINASE, CELLS, ORGAN-CULTURE, VIVO, DISEASE, EXPLANTS, GENITAL-INFECTION
journal title
PLOS ONE
PLoS One
volume
6
issue
7
article number
e22160
pages
10 pages
Web of Science type
Article
Web of Science id
000292811700030
JCR category
BIOLOGY
JCR impact factor
4.092 (2011)
JCR rank
12/84 (2011)
JCR quartile
1 (2011)
ISSN
1932-6203
DOI
10.1371/journal.pone.0022160
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1982904
handle
http://hdl.handle.net/1854/LU-1982904
date created
2012-01-11 10:51:06
date last changed
2016-12-21 15:42:28
@article{1982904,
  abstract     = {Background: Herpes simplex virus infections are highly prevalent in humans. However, the current therapeutics suffer important drawbacks such as limited results in neonates, increasing occurrence of resistance and impeded treatment of stromal infections. Remarkably, interactions of herpesviruses with human mucosa, the locus of infection, remain poorly understood and the underlying mechanisms in stromal infection remain controversial. 
Methodology/Principal Findings: A human model consisting of nasal respiratory mucosa explants was characterised. Viability and integrity were examined during 96 h of cultivation. HSV1-mucosa interactions were analysed. In particular, we investigated whether HSV1 is able to reach the stroma. Explant viability and integrity remained preserved. HSV1 induced rounding up and loosening of epithelial cells with very few apoptotic and necrotic cells observed. Following 16-24 h of infection, HSV1 penetrated the basement membrane and replicated in the underlying lamina propria. 
Conclusions/Significance: This human explant model can be used to study virus-mucosa interactions and viral mucosal invasion mechanisms. Using this model, our results provide a novel insight into the HSV1 stromal invasion mechanism and for the first time directly demonstrate that HSV1 can penetrate the basement membrane.},
  articleno    = {e22160},
  author       = {Glorieux, Sarah and Bachert, Claus and Favoreel, Herman and Vandekerckhove, Annelies and Steukers, Lennert and Rekecki, Anamaria and Van Den Broeck, Wim and Goossens, Joline and Croubels, Siska and Clayton, Reginald F and Nauwynck, Hans},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {IN-VITRO,BLOCKS APOPTOSIS,FLOW-CYTOMETRY,US3 PROTEIN-KINASE,CELLS,ORGAN-CULTURE,VIVO,DISEASE,EXPLANTS,GENITAL-INFECTION},
  language     = {eng},
  number       = {7},
  pages        = {10},
  title        = {Herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa},
  url          = {http://dx.doi.org/10.1371/journal.pone.0022160},
  volume       = {6},
  year         = {2011},
}

Chicago
GLORIEUX, SARAH, Claus Bachert, Herman Favoreel, Annelies Vandekerckhove, Lennert Steukers, Anamaria Rekecki, Wim Van Den Broeck, et al. 2011. “Herpes Simplex Virus Type 1 Penetrates the Basement Membrane in Human Nasal Respiratory Mucosa.” Plos One 6 (7).
APA
GLORIEUX, S., Bachert, C., Favoreel, H., Vandekerckhove, A., Steukers, L., Rekecki, A., Van Den Broeck, W., et al. (2011). Herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa. PLOS ONE, 6(7).
Vancouver
1.
GLORIEUX S, Bachert C, Favoreel H, Vandekerckhove A, Steukers L, Rekecki A, et al. Herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa. PLOS ONE. 2011;6(7).
MLA
GLORIEUX, SARAH, Claus Bachert, Herman Favoreel, et al. “Herpes Simplex Virus Type 1 Penetrates the Basement Membrane in Human Nasal Respiratory Mucosa.” PLOS ONE 6.7 (2011): n. pag. Print.