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Who is in there?: exploration of endophytic bacteria within the siphonous green seaweed Bryopsis (Bryopsidales, Chlorophyta)

Joke Hollants UGent, Olivier Leroux UGent, Frédérik Leliaert UGent, Helen Decleyre UGent, Olivier De Clerck UGent and Anne Willems UGent (2011) PLOS ONE. 6(10).
abstract
Associations between marine seaweeds and bacteria are widespread, with endobiotic bacterial-algal interactions being described for over 40 years. Also within the siphonous marine green alga Bryopsis, intracellular bacteria have been visualized by electron microscopy in the early '70s, but were up to now never molecularly analyzed. To study this partnership, we examined the presence and phylogenetic diversity of microbial communities within the cytoplasm of two Bryopsis species by combining fluorescence in situ hybridization (FISH), denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene clone libraries. Sequencing results revealed the presence of Arcobacter, Bacteroidetes, Flavobacteriaceae, Mycoplasma, Labrenzia, Phyllobacteriaceae and Xanthomonadaceae species. Although the total diversity of the endobiotic communities was unique to each Bryopsis culture, Bacteroidetes, Mycoplasma, Phyllobacteriaceae, and in particular Flavobacteriaceae bacteria, were detected in several Bryopsis samples collected hundreds of kilometres apart. This suggests that Bryopsis closely associates with well-defined endophytic bacterial communities of which some members possibly maintain an endosymbiotic relationship with the algal host.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
IDENTIFICATION, ABALONE, DIVERSITY, ALGA, SP-NOV., ACID BACTERIA, MARINE MACROALGAE, EPIPHYTIC BACTERIA, LAMINARIA-SACCHARINA, PHYLOGENETIC CHARACTERIZATION
journal title
PLOS ONE
PLoS One
volume
6
issue
10
article_number
e26458
pages
11 pages
Web of Science type
Article
Web of Science id
000296186900066
JCR category
BIOLOGY
JCR impact factor
4.092 (2011)
JCR rank
12/84 (2011)
JCR quartile
1 (2011)
ISSN
1932-6203
DOI
10.1371/journal.pone.0026458
project
FWO-Flanders project G.0045.08
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1981844
handle
http://hdl.handle.net/1854/LU-1981844
date created
2012-01-10 15:48:02
date last changed
2012-01-16 13:42:30
@article{1981844,
  abstract     = {Associations between marine seaweeds and bacteria are widespread, with endobiotic bacterial-algal interactions being described for over 40 years. Also within the siphonous marine green alga Bryopsis, intracellular bacteria have been visualized by electron microscopy in the early '70s, but were up to now never molecularly analyzed. To study this partnership, we examined the presence and phylogenetic diversity of microbial communities within the cytoplasm of two Bryopsis species by combining fluorescence in situ hybridization (FISH), denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene clone libraries. Sequencing results revealed the presence of Arcobacter, Bacteroidetes, Flavobacteriaceae, Mycoplasma, Labrenzia, Phyllobacteriaceae and Xanthomonadaceae species. Although the total diversity of the endobiotic communities was unique to each Bryopsis culture, Bacteroidetes, Mycoplasma, Phyllobacteriaceae, and in particular Flavobacteriaceae bacteria, were detected in several Bryopsis samples collected hundreds of kilometres apart. This suggests that Bryopsis closely associates with well-defined endophytic bacterial communities of which some members possibly maintain an endosymbiotic relationship with the algal host.},
  articleno    = {e26458},
  author       = {Hollants, Joke and Leroux, Olivier and Leliaert, Fr{\'e}d{\'e}rik and Decleyre, Helen and De Clerck, Olivier and Willems, Anne},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {IDENTIFICATION,ABALONE,DIVERSITY,ALGA,SP-NOV.,ACID BACTERIA,MARINE MACROALGAE,EPIPHYTIC BACTERIA,LAMINARIA-SACCHARINA,PHYLOGENETIC CHARACTERIZATION},
  language     = {eng},
  number       = {10},
  pages        = {11},
  title        = {Who is in there?: exploration of endophytic bacteria within the siphonous green seaweed Bryopsis (Bryopsidales, Chlorophyta)},
  url          = {http://dx.doi.org/10.1371/journal.pone.0026458},
  volume       = {6},
  year         = {2011},
}

Chicago
Hollants, Joke, Olivier Leroux, Frédérik Leliaert, Helen Decleyre, Olivier De Clerck, and Anne Willems. 2011. “Who Is in There?: Exploration of Endophytic Bacteria Within the Siphonous Green Seaweed Bryopsis (Bryopsidales, Chlorophyta).” Plos One 6 (10).
APA
Hollants, J., Leroux, O., Leliaert, F., Decleyre, H., De Clerck, O., & Willems, A. (2011). Who is in there?: exploration of endophytic bacteria within the siphonous green seaweed Bryopsis (Bryopsidales, Chlorophyta). PLOS ONE, 6(10).
Vancouver
1.
Hollants J, Leroux O, Leliaert F, Decleyre H, De Clerck O, Willems A. Who is in there?: exploration of endophytic bacteria within the siphonous green seaweed Bryopsis (Bryopsidales, Chlorophyta). PLOS ONE. 2011;6(10).
MLA
Hollants, Joke, Olivier Leroux, Frédérik Leliaert, et al. “Who Is in There?: Exploration of Endophytic Bacteria Within the Siphonous Green Seaweed Bryopsis (Bryopsidales, Chlorophyta).” PLOS ONE 6.10 (2011): n. pag. Print.