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Microbial community of predatory bugs of the genus Macrolophus (Hemiptera: Miridae)

Thijs Machtelinckx UGent, Thomas Van Leeuwen UGent, Tom Van de Wiele UGent, Nico Boon UGent, Winnok De Vos UGent, Juan-Antonio Sanchez, Mauro Nannini, Godelieve Gheysen UGent and Patrick De Clercq UGent (2012) BMC MICROBIOLOGY. 12(suppl. 1).
abstract
Background : The predatory mirids of the genus Macrolophus are key natural enemies of various economically important agricultural pests. Both M. caliginosus and M. pygmaeus are commercially available for the augmentative biological control of arthropod pests in European greenhouses. The latter species is known to be infected with Wolbachia -inducing cytoplasmic incompatibility in its host- but the presence of other endosymbionts has not been demonstrated. In the present study, the microbial diversity was examined in various populations of M. caliginosus and M. pygmaeus by 16S rRNA sequencing and denaturing gradient gel electrophoresis. Results : Besides Wolbachia, a co-infection of 2 Rickettsia species was detected in all M. pygmaeus populations. Based on a concatenated alignment of the 16S rRNA gene, the gltA gene and the coxA gene, the first is phylogenetically related to Rickettsia bellii, whereas the other is closely related to Rickettsia limoniae. All M. caliginosus populations were infected with the same Wolbachia and limoniae-like Rickettsia strain as M. pygmaeus, but did not harbour the bellii-like Rickettsia strain. Interestingly, individuals with a single infection were not found. A PCR assay on the ovaries of M. pygmaeus and M. caliginosus indicated that all endosymbionts are vertically transmitted. The presence of Wolbachia and Rickettsia in oocytes was confirmed by a fluorescence in situ hybridisation. A bio-assay comparing an infected and an uninfected M. pygmaeus population suggested that the endosymbionts had minor effects on nymphal development of their insect host and did not influence its fecundity. Conclusion : Two species of the palaearctic mirid genus Macrolophus are infected with multiple endosymbionts, including Wolbachia and Rickettsia. Independent of the origin, all tested populations of both M. pygmaeus and M. caliginosus were infected with three and two endosymbionts, respectively. There was no indication that infection with endosymbiotic bacteria had a fitness cost in terms of development and fecundity of the predators.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
16S RIBOSOMAL-RNA, GRADIENT GEL-ELECTROPHORESIS, NONCULTIVATED PLANTS, BACTEROIDETES SYMBIONT, CYTOPLASMIC INCOMPATIBILITY, BACTERIAL SYMBIONT, PARASITIC WASP, ADALIA-BIPUNCTATA, RICKETTSIA, WOLBACHIA BACTERIA
journal title
BMC MICROBIOLOGY
BMC Microbiol.
volume
12
issue
suppl. 1
article_number
S9
pages
14 pages
Web of Science type
Article
Web of Science id
000304083500009
JCR category
MICROBIOLOGY
JCR impact factor
3.104 (2012)
JCR rank
41/116 (2012)
JCR quartile
2 (2012)
ISSN
1471-2180
DOI
10.1186/1471-2180-12-S1-S9
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1979318
handle
http://hdl.handle.net/1854/LU-1979318
date created
2012-01-05 16:36:14
date last changed
2014-02-17 12:07:55
@article{1979318,
  abstract     = {Background : The predatory mirids of the genus Macrolophus are key natural enemies of various economically important agricultural pests. Both M. caliginosus and M. pygmaeus are commercially available for the augmentative biological control of arthropod pests in European greenhouses. The latter species is known to be infected with Wolbachia -inducing cytoplasmic incompatibility in its host- but the presence of other endosymbionts has not been demonstrated. In the present study, the microbial diversity was examined in various populations of M. caliginosus and M. pygmaeus by 16S rRNA sequencing and denaturing gradient gel electrophoresis.
Results : Besides Wolbachia, a co-infection of 2 Rickettsia species was detected in all M. pygmaeus populations. Based on a concatenated alignment of the 16S rRNA gene, the gltA gene and the coxA gene, the first is phylogenetically related to Rickettsia bellii, whereas the other is closely related to Rickettsia limoniae. All M. caliginosus populations were infected with the same Wolbachia and limoniae-like Rickettsia strain as M. pygmaeus, but did not harbour the bellii-like Rickettsia strain. Interestingly, individuals with a single infection were not found. A PCR assay on the ovaries of M. pygmaeus and M. caliginosus indicated that all endosymbionts are vertically transmitted. The presence of Wolbachia and Rickettsia in oocytes was confirmed by a fluorescence in situ hybridisation. A bio-assay comparing an infected and an uninfected M. pygmaeus population suggested that the endosymbionts had minor effects on nymphal development of their insect host and did not influence its fecundity.
Conclusion : Two species of the palaearctic mirid genus Macrolophus are infected with multiple endosymbionts, including Wolbachia and Rickettsia. Independent of the origin, all tested populations of both M. pygmaeus and M. caliginosus were infected with three and two endosymbionts, respectively. There was no indication that infection with endosymbiotic bacteria had a fitness cost in terms of development and fecundity of the predators.},
  articleno    = {S9},
  author       = {Machtelinckx, Thijs and Van Leeuwen, Thomas and Van de Wiele, Tom and Boon, Nico and De Vos, Winnok and Sanchez, Juan-Antonio and Nannini, Mauro and Gheysen, Godelieve and De Clercq, Patrick},
  issn         = {1471-2180},
  journal      = {BMC MICROBIOLOGY},
  keyword      = {16S RIBOSOMAL-RNA,GRADIENT GEL-ELECTROPHORESIS,NONCULTIVATED PLANTS,BACTEROIDETES SYMBIONT,CYTOPLASMIC INCOMPATIBILITY,BACTERIAL SYMBIONT,PARASITIC WASP,ADALIA-BIPUNCTATA,RICKETTSIA,WOLBACHIA BACTERIA},
  language     = {eng},
  number       = {suppl. 1},
  pages        = {14},
  title        = {Microbial community of predatory bugs of the genus Macrolophus (Hemiptera: Miridae)},
  url          = {http://dx.doi.org/10.1186/1471-2180-12-S1-S9},
  volume       = {12},
  year         = {2012},
}

Chicago
Machtelinckx, Thijs, Thomas Van Leeuwen, Tom Van de Wiele, Nico Boon, Winnok De Vos, Juan-Antonio Sanchez, Mauro Nannini, Godelieve Gheysen, and Patrick De Clercq. 2012. “Microbial Community of Predatory Bugs of the Genus Macrolophus (Hemiptera: Miridae).” Bmc Microbiology 12 (suppl. 1).
APA
Machtelinckx, T., Van Leeuwen, T., Van de Wiele, T., Boon, N., De Vos, W., Sanchez, J.-A., Nannini, M., et al. (2012). Microbial community of predatory bugs of the genus Macrolophus (Hemiptera: Miridae). BMC MICROBIOLOGY, 12(suppl. 1).
Vancouver
1.
Machtelinckx T, Van Leeuwen T, Van de Wiele T, Boon N, De Vos W, Sanchez J-A, et al. Microbial community of predatory bugs of the genus Macrolophus (Hemiptera: Miridae). BMC MICROBIOLOGY. 2012;12(suppl. 1).
MLA
Machtelinckx, Thijs, Thomas Van Leeuwen, Tom Van de Wiele, et al. “Microbial Community of Predatory Bugs of the Genus Macrolophus (Hemiptera: Miridae).” BMC MICROBIOLOGY 12.suppl. 1 (2012): n. pag. Print.