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Evaluation of five immunoassays for detection of Chlamydia psittaci in cloacal and conjunctival specimens from turkeys

Daisy Vanrompay UGent, Anne Van Nerom, Richard Ducatelle UGent and Freddy Haesebrouck UGent (1994) JOURNAL OF CLINICAL MICROBIOLOGY. 32(6). p.1470-1474
abstract
Five commercially available immunoassays were evaluated for the detection of Chlamydia psittaci in cloacal and conjunctival swabs from industrially raised turkeys: IMAGEN (DAKO Diagnostics, fly, Cambridgeshire, United Kingdom), Chlamydia CEL-VET IF (Cellabs, Brookvale, Australia), IDEIA (DAKO Diagnostics), CELISA (Cellabs), and CLEARVIEW (Unipath, Bedford, United Kingdom). Results were compared with isolation in Buffalo Green Monkey cells as a reference method. For the conjunctival samples, the sensitivities of the IMAGEN test, the Chlamydia CEL-VET IF test, the IDEIA, the CELISA, and the CLEARVIEW test were found to be 100, 66, 0, 0, and 0%, respectively, as compared to the reference test. Also for the conjunctival samples, the specificities of the IMAGEN test, the Chlamydia CEL-VET IF test, and the IDEIA were found to be 100, 11, and 92.8%, respectively. For the cloacal specimens, the sensitivities of the IMAGEN test, the Chlamydia CEL-VET IF test, the IDEIA, the CELISA, and the CLEARVIEW test were found to be 100, 93.3, 26.6, 0, and 53.3%, respectively. Also for the cloacal specimens, the specificities of the IMAGEN test, the Chlamydia CEL-VET IF test, the IDEIA, and the CLEARVIEW test were found to be 92, 12, 100, and 88%, respectively. The IMAGEN test was the most sensitive and specific direct chlamydia antigen detection test for cloacal and conjunctival samples from turkeys.
Please use this url to cite or link to this publication:
author
organization
alternative title
Evaluation of 5 immunoassays for detection of Chlamydia psittaci in cloacal and conjunctival specimens from turkeys
year
type
journalArticle (original)
publication status
published
subject
keyword
TRACHOMATIS, CLEARVIEW CHLAMYDIA, DIAGNOSIS, INFECTION, SENSITIVITY, STRAINS, URINE
journal title
JOURNAL OF CLINICAL MICROBIOLOGY
J. Clin. Microbiol.
volume
32
issue
6
pages
1470 - 1474
Web of Science type
Article
ISSN
0095-1137
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
197853
handle
http://hdl.handle.net/1854/LU-197853
alternative location
http://jcm.asm.org/content/32/6/1470.abstract
date created
2004-01-14 13:42:00
date last changed
2016-12-19 15:38:09
@article{197853,
  abstract     = {Five commercially available immunoassays were evaluated for the detection of Chlamydia psittaci in cloacal and conjunctival swabs from industrially raised turkeys: IMAGEN (DAKO Diagnostics, fly, Cambridgeshire, United Kingdom), Chlamydia CEL-VET IF (Cellabs, Brookvale, Australia), IDEIA (DAKO Diagnostics), CELISA (Cellabs), and CLEARVIEW (Unipath, Bedford, United Kingdom). Results were compared with isolation in Buffalo Green Monkey cells as a reference method. For the conjunctival samples, the sensitivities of the IMAGEN test, the Chlamydia CEL-VET IF test, the IDEIA, the CELISA, and the CLEARVIEW test were found to be 100, 66, 0, 0, and 0\%, respectively, as compared to the reference test. Also for the conjunctival samples, the specificities of the IMAGEN test, the Chlamydia CEL-VET IF test, and the IDEIA were found to be 100, 11, and 92.8\%, respectively. For the cloacal specimens, the sensitivities of the IMAGEN test, the Chlamydia CEL-VET IF test, the IDEIA, the CELISA, and the CLEARVIEW test were found to be 100, 93.3, 26.6, 0, and 53.3\%, respectively. Also for the cloacal specimens, the specificities of the IMAGEN test, the Chlamydia CEL-VET IF test, the IDEIA, and the CLEARVIEW test were found to be 92, 12, 100, and 88\%, respectively. The IMAGEN test was the most sensitive and specific direct chlamydia antigen detection test for cloacal and conjunctival samples from turkeys.},
  author       = {Vanrompay, Daisy and Van Nerom, Anne and Ducatelle, Richard and Haesebrouck, Freddy},
  issn         = {0095-1137},
  journal      = {JOURNAL OF CLINICAL MICROBIOLOGY},
  keyword      = {TRACHOMATIS,CLEARVIEW CHLAMYDIA,DIAGNOSIS,INFECTION,SENSITIVITY,STRAINS,URINE},
  language     = {eng},
  number       = {6},
  pages        = {1470--1474},
  title        = {Evaluation of five immunoassays for detection of Chlamydia psittaci in cloacal and conjunctival specimens from turkeys},
  url          = {http://jcm.asm.org/content/32/6/1470.abstract},
  volume       = {32},
  year         = {1994},
}

Chicago
Vanrompay, Daisy, Anne Van Nerom, Richard Ducatelle, and Freddy Haesebrouck. 1994. “Evaluation of Five Immunoassays for Detection of Chlamydia Psittaci in Cloacal and Conjunctival Specimens from Turkeys.” Journal of Clinical Microbiology 32 (6): 1470–1474.
APA
Vanrompay, D., Van Nerom, A., Ducatelle, R., & Haesebrouck, F. (1994). Evaluation of five immunoassays for detection of Chlamydia psittaci in cloacal and conjunctival specimens from turkeys. JOURNAL OF CLINICAL MICROBIOLOGY, 32(6), 1470–1474.
Vancouver
1.
Vanrompay D, Van Nerom A, Ducatelle R, Haesebrouck F. Evaluation of five immunoassays for detection of Chlamydia psittaci in cloacal and conjunctival specimens from turkeys. JOURNAL OF CLINICAL MICROBIOLOGY. 1994;32(6):1470–4.
MLA
Vanrompay, Daisy, Anne Van Nerom, Richard Ducatelle, et al. “Evaluation of Five Immunoassays for Detection of Chlamydia Psittaci in Cloacal and Conjunctival Specimens from Turkeys.” JOURNAL OF CLINICAL MICROBIOLOGY 32.6 (1994): 1470–1474. Print.