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Modification of competence for in vitro response to Fusarium oxysponim in tomato cells, II: effect of the integration of Agrobacterium tumefaciens genes for auxin and cytokinin synthesis

E Storti, P Bogani, P Bettini, P Bittini, ML Guardiola, MG Pellegrini, Dirk Inzé UGent and M Buiatti (1994) THEORETICAL AND APPLIED GENETICS. 88(1). p.89-96
abstract
We have studied the effect of a change in the endogenous hormone equilibria on the competence of tomato (Lycopersicon esculentum) cells to defend themselves against the fungal pathogen Fusarium oxysporum f. sp. lycopersici. Calluses from cvs 'Davis' and 'Red River', respectively resistant and susceptible to Fusarium and transgenic for an auxin- or cytokinin-synthesizing gene from Agrobacterium tumefaciens, were used. The integration of Agrobacterium hormone-related genes into susceptible cv 'Red River' can bring the activation of defense processes to a stable competence as assessed by the inhibition of mycelial growth in dual culture and gem-tube elongation of Fusarium conidia, the determination of callose contents, peroxidase induction and ion leakage in the presence of fusaric acid. This is particularly true when the transformation results in a change of phytohormone equilibria towards an higher cytokin in concentration. On the contrary, in resistant cv 'Davis' the inhibition of both fungal growth in dual culture and conidia germination is higher when the hormone balance is modified in favour of the auxins. No significant effect was observed for ion leakage and peroxidase induction, probably because of a constitutive overproduction of cytokinins in 'Davis' cells.
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alternative title
Modification of competence for in vitro response to Fusarium oxysponim in tomato cells, 2 : effect of the integration of Agrobacterium tumefaciens genes for auxin and cytokinin synthesis
year
type
journalArticle (original)
publication status
published
subject
keyword
FUSARIUM OXYSPORUM F SP LYCOPERSICI, LYCOPERSICON ESCULENTUM, AGROBACTERIUM HORMONE SYNTHETIC GENES, DEFENSE RESPONSE, SYSTEMIC ACQUIRED-RESISTANCE, PATHOGENESIS-RELATED PROTEINS, CULTURED TOBACCO TISSUES, F SP LYCOPERSICI, ALTERED RESPONSE, WALL COMPONENTS, MOSAIC-VIRUS, T-DNA, PLANTS, PHENOTYPE
journal title
THEORETICAL AND APPLIED GENETICS
Theor. Appl. Genet.
volume
88
issue
1
pages
89 - 96
Web of Science type
Article
ISSN
0040-5752
DOI
10.1007/BF00222399
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
197742
handle
http://hdl.handle.net/1854/LU-197742
date created
2004-01-14 13:42:00
date last changed
2016-12-19 15:39:03
@article{197742,
  abstract     = {We have studied the effect of a change in the endogenous hormone equilibria on the competence of tomato (Lycopersicon esculentum) cells to defend themselves against the fungal pathogen Fusarium oxysporum f. sp. lycopersici. Calluses from cvs 'Davis' and 'Red River', respectively resistant and susceptible to Fusarium and transgenic for an auxin- or cytokinin-synthesizing gene from Agrobacterium tumefaciens, were used. The integration of Agrobacterium hormone-related genes into susceptible cv 'Red River' can bring the activation of defense processes to a stable competence as assessed by the inhibition of mycelial growth in dual culture and gem-tube elongation of Fusarium conidia, the determination of callose contents, peroxidase induction and ion leakage in the presence of fusaric acid. This is particularly true when the transformation results in a change of phytohormone equilibria towards an higher cytokin in concentration. On the contrary, in resistant cv 'Davis' the inhibition of both fungal growth in dual culture and conidia germination is higher when the hormone balance is modified in favour of the auxins. No significant effect was observed for ion leakage and peroxidase induction, probably because of a constitutive overproduction of cytokinins in 'Davis' cells.},
  author       = {Storti, E and Bogani, P and Bettini, P and Bittini, P and Guardiola, ML and Pellegrini, MG and Inz{\'e}, Dirk and Buiatti, M},
  issn         = {0040-5752},
  journal      = {THEORETICAL AND APPLIED GENETICS},
  keyword      = {FUSARIUM OXYSPORUM F SP LYCOPERSICI,LYCOPERSICON ESCULENTUM,AGROBACTERIUM HORMONE SYNTHETIC GENES,DEFENSE RESPONSE,SYSTEMIC ACQUIRED-RESISTANCE,PATHOGENESIS-RELATED PROTEINS,CULTURED TOBACCO TISSUES,F SP LYCOPERSICI,ALTERED RESPONSE,WALL COMPONENTS,MOSAIC-VIRUS,T-DNA,PLANTS,PHENOTYPE},
  language     = {eng},
  number       = {1},
  pages        = {89--96},
  title        = {Modification of competence for in vitro response to Fusarium oxysponim in tomato cells, II: effect of the integration of Agrobacterium tumefaciens genes for auxin and cytokinin synthesis},
  url          = {http://dx.doi.org/10.1007/BF00222399},
  volume       = {88},
  year         = {1994},
}

Chicago
Storti, E, P Bogani, P Bettini, P Bittini, ML Guardiola, MG Pellegrini, Dirk Inzé, and M Buiatti. 1994. “Modification of Competence for in Vitro Response to Fusarium Oxysponim in Tomato Cells, II: Effect of the Integration of Agrobacterium Tumefaciens Genes for Auxin and Cytokinin Synthesis.” Theoretical and Applied Genetics 88 (1): 89–96.
APA
Storti, E., Bogani, P., Bettini, P., Bittini, P., Guardiola, M., Pellegrini, M., Inzé, D., et al. (1994). Modification of competence for in vitro response to Fusarium oxysponim in tomato cells, II: effect of the integration of Agrobacterium tumefaciens genes for auxin and cytokinin synthesis. THEORETICAL AND APPLIED GENETICS, 88(1), 89–96.
Vancouver
1.
Storti E, Bogani P, Bettini P, Bittini P, Guardiola M, Pellegrini M, et al. Modification of competence for in vitro response to Fusarium oxysponim in tomato cells, II: effect of the integration of Agrobacterium tumefaciens genes for auxin and cytokinin synthesis. THEORETICAL AND APPLIED GENETICS. 1994;88(1):89–96.
MLA
Storti, E, P Bogani, P Bettini, et al. “Modification of Competence for in Vitro Response to Fusarium Oxysponim in Tomato Cells, II: Effect of the Integration of Agrobacterium Tumefaciens Genes for Auxin and Cytokinin Synthesis.” THEORETICAL AND APPLIED GENETICS 88.1 (1994): 89–96. Print.