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Development of a molecularly imprinted polymer for patulin in apple juice

David De Smet UGent, Peter Dubruel UGent, Carlos Van Peteghem UGent and Sarah De Saeger UGent (2011) WORLD MYCOTOXIN JOURNAL. 4(4). p.375-383
abstract
The design of imprinted polymers selective towards patulin (PAT) and their application in food analysis are reported for the first time. Different templates, functional monomers and molar ratios were evaluated related to binding capacity and specificity. Besides the toxin itself, the implementation of structural analogues (2-hydroxynicotinic acid, 5-indanol and 3-hydroxyphtalic anhydride) as templates was evaluated. A molecularly imprinted solid-phase extraction (MISPE) procedure was optimised for the selective clean-up of apple juice samples. Depending on the spiked concentration, recoveries after MISPE and non-imprinted solid-phase extraction varied respectively from 60% to 66% and from 40% to 41%. Limit of detection and limit of quantification were 10.0 mu g/kg and 33.3 mu g/kg, respectively. Equilibrium experiments and Scatchard analysis disclosed the presence of two classes of binding sites in the imprinted polymer. The dissociation constant (K(D)) of the higher affinity binding sites was 3.3 mu mol/l, while the K(D) of the lower affinity binding sites was 260.7 mu mol/l. The performance of the molecularly imprinted polymer throughout the clean-up was compared to liquid-liquid extraction and a C(18) sorbent. Cross-reactivity experiments demonstrated that MISPE was substantially more selective than C(18) clean-up. Moreover chromatograms, with less interfering peaks, were observed with MISPE resulting in a sensitive and reliable quantification of PAT.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
molecular recognition, SOLID-PHASE EXTRACTION, molecularly imprinted solid-phase extraction, apple juice, patulin, CHROMATOGRAPHIC METHOD, LIQUID-CHROMATOGRAPHY, PENICILLIUM-EXPANSUM, MASS-SPECTROMETRY, HPLC-DAD, FOOD, ZEARALENONE, MYCOTOXINS, PRODUCTS
journal title
WORLD MYCOTOXIN JOURNAL
World Mycotoxin J.
volume
4
issue
4
pages
375 - 383
Web of Science type
Article
Web of Science id
000297061900003
JCR category
MYCOLOGY
JCR impact factor
1.452 (2011)
JCR rank
15/24 (2011)
JCR quartile
3 (2011)
ISSN
1875-0710
DOI
10.3920/WMJ2010.1276
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1977015
handle
http://hdl.handle.net/1854/LU-1977015
date created
2012-01-03 14:30:52
date last changed
2012-01-16 10:26:38
@article{1977015,
  abstract     = {The design of imprinted polymers selective towards patulin (PAT) and their application in food analysis are reported for the first time. Different templates, functional monomers and molar ratios were evaluated related to binding capacity and specificity. Besides the toxin itself, the implementation of structural analogues (2-hydroxynicotinic acid, 5-indanol and 3-hydroxyphtalic anhydride) as templates was evaluated. A molecularly imprinted solid-phase extraction (MISPE) procedure was optimised for the selective clean-up of apple juice samples. Depending on the spiked concentration, recoveries after MISPE and non-imprinted solid-phase extraction varied respectively from 60\% to 66\% and from 40\% to 41\%. Limit of detection and limit of quantification were 10.0 mu g/kg and 33.3 mu g/kg, respectively. Equilibrium experiments and Scatchard analysis disclosed the presence of two classes of binding sites in the imprinted polymer. The dissociation constant (K(D)) of the higher affinity binding sites was 3.3 mu mol/l, while the K(D) of the lower affinity binding sites was 260.7 mu mol/l. The performance of the molecularly imprinted polymer throughout the clean-up was compared to liquid-liquid extraction and a C(18) sorbent. Cross-reactivity experiments demonstrated that MISPE was substantially more selective than C(18) clean-up. Moreover chromatograms, with less interfering peaks, were observed with MISPE resulting in a sensitive and reliable quantification of PAT.},
  author       = {De Smet, David and Dubruel, Peter and Van Peteghem, Carlos and De Saeger, Sarah},
  issn         = {1875-0710},
  journal      = {WORLD MYCOTOXIN JOURNAL},
  keyword      = {molecular recognition,SOLID-PHASE EXTRACTION,molecularly imprinted solid-phase extraction,apple juice,patulin,CHROMATOGRAPHIC METHOD,LIQUID-CHROMATOGRAPHY,PENICILLIUM-EXPANSUM,MASS-SPECTROMETRY,HPLC-DAD,FOOD,ZEARALENONE,MYCOTOXINS,PRODUCTS},
  language     = {eng},
  number       = {4},
  pages        = {375--383},
  title        = {Development of a molecularly imprinted polymer for patulin in apple juice},
  url          = {http://dx.doi.org/10.3920/WMJ2010.1276},
  volume       = {4},
  year         = {2011},
}

Chicago
De Smet, David, Peter Dubruel, Carlos Van Peteghem, and Sarah De Saeger. 2011. “Development of a Molecularly Imprinted Polymer for Patulin in Apple Juice.” World Mycotoxin Journal 4 (4): 375–383.
APA
De Smet, David, Dubruel, P., Van Peteghem, C., & De Saeger, S. (2011). Development of a molecularly imprinted polymer for patulin in apple juice. WORLD MYCOTOXIN JOURNAL, 4(4), 375–383.
Vancouver
1.
De Smet D, Dubruel P, Van Peteghem C, De Saeger S. Development of a molecularly imprinted polymer for patulin in apple juice. WORLD MYCOTOXIN JOURNAL. 2011;4(4):375–83.
MLA
De Smet, David, Peter Dubruel, Carlos Van Peteghem, et al. “Development of a Molecularly Imprinted Polymer for Patulin in Apple Juice.” WORLD MYCOTOXIN JOURNAL 4.4 (2011): 375–383. Print.