Advanced search
1 file | 246.19 KB Add to list

Development and validation of an UPLC-MS/MS method for the determination of ionophoric and synthetic coccidiostats in vegetables

Author
Organization
Abstract
In poultry farming, anticoccidial drugs are widely used as feed additives for the prevention and treatment of coccidiosis. Because coccidiostats and veterinary medicines, in general, are often poorly absorbed, manure from treated animals may contain high concentrations of these compounds. Experimental studies have shown that the uptake of veterinary medicines by plants from soil containing contaminated manure may occur. This leads to several questions regarding the impact on the environment, resistance problems, and public health and allergy issues. This work describes the development of a quantification method for coccidiostats in vegetables. Vegetables were spiked at 100 mu g kg(-1) (dry weight) with coccidiostats (monensin, narasin, lasalocid A, salinomycin, diclazuril, and nicarbazin) in order to optimize the extraction and clean-up. Possible critical factors (e.g., extraction solvent) were statistically examined by linear regression with the use of Plackett-Burman and full factorial designs. Final extracts were analyzed with ultra-performance liquid chromatography tandem mass spectrometry operating in multiple-reaction monitoring mode. Both the synthetic and ionophoric coccidiostats could be determined in a single run with an analysis time of 5 min. The developed method was validated taking into account the requirements of the Commission Decision 2002/657/EC as a guideline. The method is regarded as applicable for its intended purposes with quantification limits between 0.30 and 2.98 mu g kg(-1). This method could be used to establish possible maximum residue limits for coccidiostats in vegetables, as already exist for eggs, meat, and milk.
Keywords
ANIMAL MANURE, SOIL, MASS-SPECTROMETRY, Veterinary, Residue, VETERINARY ANTIBIOTICS, Vegetables, Coccidiostats, LC-MS/MS, MONENSIN, ENVIRONMENT, MEDICINES, PLANTS, WATER, QUANTIFICATION

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 246.19 KB

Citation

Please use this url to cite or link to this publication:

MLA
Broekaert, Nathan, et al. “Development and Validation of an UPLC-MS/MS Method for the Determination of Ionophoric and Synthetic Coccidiostats in Vegetables.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY, vol. 401, no. 10, 2011, pp. 3335–44, doi:10.1007/s00216-011-5433-1.
APA
Broekaert, N., Van Peteghem, C., Daeseleire, E., Sticker, D., & Van Poucke, C. (2011). Development and validation of an UPLC-MS/MS method for the determination of ionophoric and synthetic coccidiostats in vegetables. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 401(10), 3335–3344. https://doi.org/10.1007/s00216-011-5433-1
Chicago author-date
Broekaert, Nathan, Carlos Van Peteghem, Els Daeseleire, David Sticker, and Christof Van Poucke. 2011. “Development and Validation of an UPLC-MS/MS Method for the Determination of Ionophoric and Synthetic Coccidiostats in Vegetables.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 401 (10): 3335–44. https://doi.org/10.1007/s00216-011-5433-1.
Chicago author-date (all authors)
Broekaert, Nathan, Carlos Van Peteghem, Els Daeseleire, David Sticker, and Christof Van Poucke. 2011. “Development and Validation of an UPLC-MS/MS Method for the Determination of Ionophoric and Synthetic Coccidiostats in Vegetables.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 401 (10): 3335–3344. doi:10.1007/s00216-011-5433-1.
Vancouver
1.
Broekaert N, Van Peteghem C, Daeseleire E, Sticker D, Van Poucke C. Development and validation of an UPLC-MS/MS method for the determination of ionophoric and synthetic coccidiostats in vegetables. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2011;401(10):3335–44.
IEEE
[1]
N. Broekaert, C. Van Peteghem, E. Daeseleire, D. Sticker, and C. Van Poucke, “Development and validation of an UPLC-MS/MS method for the determination of ionophoric and synthetic coccidiostats in vegetables,” ANALYTICAL AND BIOANALYTICAL CHEMISTRY, vol. 401, no. 10, pp. 3335–3344, 2011.
@article{1976941,
  abstract     = {{In poultry farming, anticoccidial drugs are widely used as feed additives for the prevention and treatment of coccidiosis. Because coccidiostats and veterinary medicines, in general, are often poorly absorbed, manure from treated animals may contain high concentrations of these compounds. Experimental studies have shown that the uptake of veterinary medicines by plants from soil containing contaminated manure may occur. This leads to several questions regarding the impact on the environment, resistance problems, and public health and allergy issues. This work describes the development of a quantification method for coccidiostats in vegetables. Vegetables were spiked at 100 mu g kg(-1) (dry weight) with coccidiostats (monensin, narasin, lasalocid A, salinomycin, diclazuril, and nicarbazin) in order to optimize the extraction and clean-up. Possible critical factors (e.g., extraction solvent) were statistically examined by linear regression with the use of Plackett-Burman and full factorial designs. Final extracts were analyzed with ultra-performance liquid chromatography tandem mass spectrometry operating in multiple-reaction monitoring mode. Both the synthetic and ionophoric coccidiostats could be determined in a single run with an analysis time of 5 min. The developed method was validated taking into account the requirements of the Commission Decision 2002/657/EC as a guideline. The method is regarded as applicable for its intended purposes with quantification limits between 0.30 and 2.98 mu g kg(-1). This method could be used to establish possible maximum residue limits for coccidiostats in vegetables, as already exist for eggs, meat, and milk.}},
  author       = {{Broekaert, Nathan and Van Peteghem, Carlos and Daeseleire, Els and Sticker, David and Van Poucke, Christof}},
  issn         = {{1618-2642}},
  journal      = {{ANALYTICAL AND BIOANALYTICAL CHEMISTRY}},
  keywords     = {{ANIMAL MANURE,SOIL,MASS-SPECTROMETRY,Veterinary,Residue,VETERINARY ANTIBIOTICS,Vegetables,Coccidiostats,LC-MS/MS,MONENSIN,ENVIRONMENT,MEDICINES,PLANTS,WATER,QUANTIFICATION}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{3335--3344}},
  title        = {{Development and validation of an UPLC-MS/MS method for the determination of ionophoric and synthetic coccidiostats in vegetables}},
  url          = {{http://doi.org/10.1007/s00216-011-5433-1}},
  volume       = {{401}},
  year         = {{2011}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: