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Fetal microchimeric cells in blood of women with an autoimmune thyroid disease

Trees Lepez UGent, Mado Vandewoestyne UGent, Shahid Hussain UGent, Filip Van Nieuwerburgh UGent, Kris Poppe, Brigitte Velkeniers, Jean Kaufman UGent and Dieter Deforce UGent (2011) PLOS ONE. 6(12).
abstract
Context: Hashimoto's thyroiditis (HT) and Graves' disease (GD), two autoimmune thyroid diseases (AITD), occur more frequently in women than in men and show an increased incidence in the years following parturition. Persisting fetal cells could play a role in the development of these diseases. Objective: Aim of this study was to detect and characterize fetal cells in blood of postpartum women with and without an AITD. Participants: Eleven patients with an AITD and ten healthy volunteers, all given birth to a son maximum 5 years before analysis, and three women who never had been pregnant, were included. None of them had any other disease of the thyroid which could interfere with the results obtained. Methods: Fluorescence in situ hybridization (FISH) and repeated FISH were used to count the number of male fetal cells. Furthermore, the fetal cells were further characterized. Results: In patients with HT, 7 to 11 fetal cells per 1.000.000 maternal cells were detected, compared to 14 to 29 fetal cells in patients with GD (p = 0,0061). In patients with HT, mainly fetal CD8+ T cells were found, while in patients with GD, fetal B and CD4+ T cells were detected. In healthy volunteers with son, 0 to 5 fetal cells were observed, which was significantly less than the number observed in patients (p<0,05). In women who never had been pregnant, no male cells were detected. Conclusion: This study shows a clear association between fetal microchimeric cells and autoimmune thyroid diseases.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Graves' Disease, Fetal Microchimerism, Hashimoto's thyroiditis, IN-SITU HYBRIDIZATION, HASHIMOTOS-THYROIDITIS, GRAVES-DISEASE, SYSTEMIC-SCLEROSIS, PERIPHERAL-BLOOD, POSTPARTUM, LONG, DNA
journal title
PLOS ONE
PLoS One
volume
6
issue
12
article_number
e29646
pages
8 pages
Web of Science type
Article
Web of Science id
000300674900049
JCR category
BIOLOGY
JCR impact factor
4.092 (2011)
JCR rank
12/84 (2011)
JCR quartile
1 (2011)
ISSN
1932-6203
DOI
10.1371/journal.pone.0029646
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1976629
handle
http://hdl.handle.net/1854/LU-1976629
date created
2012-01-03 09:37:00
date last changed
2012-09-17 15:34:24
@article{1976629,
  abstract     = {Context: Hashimoto's thyroiditis (HT) and Graves' disease (GD), two autoimmune thyroid diseases (AITD), occur more frequently in women than in men and show an increased incidence in the years following parturition. Persisting fetal cells could play a role in the development of these diseases.
Objective: Aim of this study was to detect and characterize fetal cells in blood of postpartum women with and without an AITD.
Participants: Eleven patients with an AITD and ten healthy volunteers, all given birth to a son maximum 5 years before analysis, and three women who never had been pregnant, were included. None of them had any other disease of the thyroid which could interfere with the results obtained.
Methods: Fluorescence in situ hybridization (FISH) and repeated FISH were used to count the number of male fetal cells. Furthermore, the fetal cells were further characterized.
Results: In patients with HT, 7 to 11 fetal cells per 1.000.000 maternal cells were detected, compared to 14 to 29 fetal cells in patients with GD (p = 0,0061). In patients with HT, mainly fetal CD8+ T cells were found, while in patients with GD, fetal B and CD4+ T cells were detected. In healthy volunteers with son, 0 to 5 fetal cells were observed, which was significantly less than the number observed in patients (p{\textlangle}0,05). In women who never had been pregnant, no male cells were detected.
Conclusion: This study shows a clear association between fetal microchimeric cells and autoimmune thyroid diseases.},
  articleno    = {e29646},
  author       = {Lepez, Trees and Vandewoestyne, Mado and Hussain, Shahid and Van Nieuwerburgh, Filip and Poppe, Kris and Velkeniers, Brigitte and Kaufman, Jean and Deforce, Dieter},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {Graves' Disease,Fetal Microchimerism,Hashimoto's thyroiditis,IN-SITU HYBRIDIZATION,HASHIMOTOS-THYROIDITIS,GRAVES-DISEASE,SYSTEMIC-SCLEROSIS,PERIPHERAL-BLOOD,POSTPARTUM,LONG,DNA},
  language     = {eng},
  number       = {12},
  pages        = {8},
  title        = {Fetal microchimeric cells in blood of women with an autoimmune thyroid disease},
  url          = {http://dx.doi.org/10.1371/journal.pone.0029646},
  volume       = {6},
  year         = {2011},
}

Chicago
Lepez, Trees, Mado Vandewoestyne, Shahid Hussain, Filip Van Nieuwerburgh, Kris Poppe, Brigitte Velkeniers, Jean Kaufman, and Dieter Deforce. 2011. “Fetal Microchimeric Cells in Blood of Women with an Autoimmune Thyroid Disease.” Plos One 6 (12).
APA
Lepez, T., Vandewoestyne, M., Hussain, S., Van Nieuwerburgh, F., Poppe, K., Velkeniers, B., Kaufman, J., et al. (2011). Fetal microchimeric cells in blood of women with an autoimmune thyroid disease. PLOS ONE, 6(12).
Vancouver
1.
Lepez T, Vandewoestyne M, Hussain S, Van Nieuwerburgh F, Poppe K, Velkeniers B, et al. Fetal microchimeric cells in blood of women with an autoimmune thyroid disease. PLOS ONE. 2011;6(12).
MLA
Lepez, Trees, Mado Vandewoestyne, Shahid Hussain, et al. “Fetal Microchimeric Cells in Blood of Women with an Autoimmune Thyroid Disease.” PLOS ONE 6.12 (2011): n. pag. Print.