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Effects of ionizing radiation on the size distribution of proteoglycan aggregates synthesized by chondrocytes in agarose

Maria Cornelissen UGent, Hubert Thierens UGent and Leo De Ridder (1993) SCANNING MICROSCOPY. 7(4). p.1263-1268
abstract
Effects of ionizing radiation on the structure and size-distribution of newly synthesized proteoglycan aggregates are studied in-vitro. Chondrocytes, isolated from embryonic chick sterna, are cultured for 7 days in a tri-dimensional agarose system. Single radiation doses of 10, 20 and 50 Gy are given before starting the culture. Digestion of the artificial agarose matrix liberates the newly synthesized proteoglycans. Spreading with cytochrome C allows electron microscopic investigations of the individual, newly synthesized molecules. The structure of aggregates synthesized by control and irradiated chondrocytes is comparable, However, radiation causes alterations in the size-distributions of the aggregate-populations. For the control cultures, an average aggregate contains 27 aggrecans per aggregate. 34 pro mille of the molecules contain more than 100 aggrecans per aggregate. Irradiation with 10 Gy doesn't cause alterations. With radiation doses of 20 and 50 Gy, an average molecule contains 20 aggrecans. Only about 9 pro mille of the aggregates contain more than 100 aggrecans. Stimulation of lysosomal activity after irradiation could explain the observed alterations.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
IONIZING RADIATION, PROTEOGLYCAN AGGREGATES, AGAROSE CULTURE, EMBRYONIC CARTILAGE, CARTILAGE
journal title
SCANNING MICROSCOPY
Scanning Microsc.
volume
7
issue
4
pages
1263 - 1268
Web of Science type
Article
ISSN
0891-7035
language
English
UGent publication?
yes
classification
A1
id
197655
handle
http://hdl.handle.net/1854/LU-197655
date created
2004-01-14 13:42:00
date last changed
2016-12-19 15:39:02
@article{197655,
  abstract     = {Effects of ionizing radiation on the structure and size-distribution of newly synthesized proteoglycan aggregates are studied in-vitro. Chondrocytes, isolated from embryonic chick sterna, are cultured for 7 days in a tri-dimensional agarose system. Single radiation doses of 10, 20 and 50 Gy are given before starting the culture. Digestion of the artificial agarose matrix liberates the newly synthesized proteoglycans. Spreading with cytochrome C allows electron microscopic investigations of the individual, newly synthesized molecules. 
The structure of aggregates synthesized by control and irradiated chondrocytes is comparable, However, radiation causes alterations in the size-distributions of the aggregate-populations. For the control cultures, an average aggregate contains 27 aggrecans per aggregate. 34 pro mille of the molecules contain more than 100 aggrecans per aggregate. Irradiation with 10 Gy doesn't cause alterations. With radiation doses of 20 and 50 Gy, an average molecule contains 20 aggrecans. Only about 9 pro mille of the aggregates contain more than 100 aggrecans. Stimulation of lysosomal activity after irradiation could explain the observed alterations.},
  author       = {Cornelissen, Maria and Thierens, Hubert and De Ridder, Leo},
  issn         = {0891-7035},
  journal      = {SCANNING MICROSCOPY},
  keyword      = {IONIZING RADIATION,PROTEOGLYCAN AGGREGATES,AGAROSE CULTURE,EMBRYONIC CARTILAGE,CARTILAGE},
  language     = {eng},
  number       = {4},
  pages        = {1263--1268},
  title        = {Effects of ionizing radiation on the size distribution of proteoglycan aggregates synthesized by chondrocytes in agarose},
  volume       = {7},
  year         = {1993},
}

Chicago
Cornelissen, Maria, Hubert Thierens, and Leo De Ridder. 1993. “Effects of Ionizing Radiation on the Size Distribution of Proteoglycan Aggregates Synthesized by Chondrocytes in Agarose.” Scanning Microscopy 7 (4): 1263–1268.
APA
Cornelissen, M., Thierens, H., & De Ridder, L. (1993). Effects of ionizing radiation on the size distribution of proteoglycan aggregates synthesized by chondrocytes in agarose. SCANNING MICROSCOPY, 7(4), 1263–1268.
Vancouver
1.
Cornelissen M, Thierens H, De Ridder L. Effects of ionizing radiation on the size distribution of proteoglycan aggregates synthesized by chondrocytes in agarose. SCANNING MICROSCOPY. 1993;7(4):1263–8.
MLA
Cornelissen, Maria, Hubert Thierens, and Leo De Ridder. “Effects of Ionizing Radiation on the Size Distribution of Proteoglycan Aggregates Synthesized by Chondrocytes in Agarose.” SCANNING MICROSCOPY 7.4 (1993): 1263–1268. Print.