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Influence of S-nitrosothiols and nitrate tolerance in the rat gastric fundus

Ann Barbier and Romain Lefebvre UGent (1994) BRITISH JOURNAL OF PHARMACOLOGY. 111(4). p.1280-1286
abstract
1 The relaxant responses of S-nitroso-L-cysteine (CysNO), S-nitroso-N-acetyl-D,L-penicillamine (SNAP), S-nitroso-N-acetyl-L-cysteine (SNAC) and S-nitrosoglutathione (GSNO) in the rat gastric fundus (forestomach) were studied and compared to the relaxant responses obtained in response to nitric oxide (NO) and electrical field stimulation (EFS, 10 s trains) of non-adrenergic non-cholinergic (NANC) nerves. 2 CysNO (10(-7)-3 x 10(-4) M) caused transient relaxation of the precontracted rat gastric fundus, comparable to the response to NO (10(-6)-10(-4) M) and EFS. SNAP, SNAC and GSNO elicited more sustained relaxations. 3 The cyclic GMP-specific phosphodiesterase inhibitor, zaprinast (3 x 10(-5) M), increased the relaxant effect of CysNO, SNAP and GSNO while the NO-synthase inhibitor, N(G)-nitro-L-arginine (L-NOARG, 3 x 10(-4) M) had no influence. 4 In the presence of LY 83583 (10(-5) M), which releases superoxide anions, the relaxant response to NO and CysNO was decreased, whereas that to all other stimuli was unaltered. The inhibitory effect of LY 83583 on CysNO-induced relaxations was prevented by superoxide dismutase (SOD, 1000 u ml-1). 5 Tissues incubated for 1 h with 5.5 x 10(-4) M nitroglycerin (GTN) became tolerant to GTN. In this condition, the relaxant response to 10(-5) M NO was maintained, while the relaxations by EFS (8 Hz) and 3 x 10(-5) M SNAP were significantly decreased. The reduction of the response to the other S-nitrosothiols was not significant. 6 The combination of nitrate tolerance and 10(-5) M LY 83583 caused a significantly larger inhibition of the relaxant response to EFS (8 Hz) than nitrate tolerance alone. The combination of LY 83583 and GTN tolerance reduced the relaxant effect of 10(-5) M NO to a similar extent to LY 83583 alone, while the relaxant response to 10(-4) M GTN was reduced to the same extent as after 1 h exposure to 5.5 x 10(-4) M GTN alone. 7 It is concluded that S-nitrosothiols potently relax the rat gastric fundus, possibly by a cyclic GMP-dependent mechanism and S-nitrosothiols such as SNAC and GSNO may be involved in NANC neurotransmission.
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author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ORGANIC NITRATES, ENDOTHELIUM, NONCHOLINERGIC RELAXATION, RELAXING FACTOR, NITRIC-OXIDE, VASCULAR SMOOTH-MUSCLE, NANC NERVE-STIMULATION, VASOACTIVE INTESTINAL POLYPEPTIDE, NITRATE TOLERANCE, NONADRENERGIC NONCHOLINERGIC (NANC) NERVES, S-NITROSOTHIOLS, RAT GASTRIC FUNDUS, MECHANISM, RELEASE
journal title
BRITISH JOURNAL OF PHARMACOLOGY
Br. J. Pharmacol.
volume
111
issue
4
pages
1280 - 1286
Web of Science type
Article
ISSN
0007-1188
DOI
10.1111/j.1476-5381.1994.tb14884.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
197287
handle
http://hdl.handle.net/1854/LU-197287
date created
2004-01-14 13:42:00
date last changed
2016-12-19 15:38:59
@article{197287,
  abstract     = {1 The relaxant responses of S-nitroso-L-cysteine (CysNO), S-nitroso-N-acetyl-D,L-penicillamine (SNAP), S-nitroso-N-acetyl-L-cysteine (SNAC) and S-nitrosoglutathione (GSNO) in the rat gastric fundus (forestomach) were studied and compared to the relaxant responses obtained in response to nitric oxide (NO) and electrical field stimulation (EFS, 10 s trains) of non-adrenergic non-cholinergic (NANC) nerves.
2 CysNO (10(-7)-3 x 10(-4) M) caused transient relaxation of the precontracted rat gastric fundus, comparable to the response to NO (10(-6)-10(-4) M) and EFS. SNAP, SNAC and GSNO elicited more sustained relaxations.
3 The cyclic GMP-specific phosphodiesterase inhibitor, zaprinast (3 x 10(-5) M), increased the relaxant effect of CysNO, SNAP and GSNO while the NO-synthase inhibitor, N(G)-nitro-L-arginine (L-NOARG, 3 x 10(-4) M) had no influence.
4 In the presence of LY 83583 (10(-5) M), which releases superoxide anions, the relaxant response to NO and CysNO was decreased, whereas that to all other stimuli was unaltered. The inhibitory effect of LY 83583 on CysNO-induced relaxations was prevented by superoxide dismutase (SOD, 1000 u ml-1).
5 Tissues incubated for 1 h with 5.5 x 10(-4) M nitroglycerin (GTN) became tolerant to GTN. In this condition, the relaxant response to 10(-5) M NO was maintained, while the relaxations by EFS (8 Hz) and 3 x 10(-5) M SNAP were significantly decreased. The reduction of the response to the other S-nitrosothiols was not significant.
6 The combination of nitrate tolerance and 10(-5) M LY 83583 caused a significantly larger inhibition of the relaxant response to EFS (8 Hz) than nitrate tolerance alone. The combination of LY 83583 and GTN tolerance reduced the relaxant effect of 10(-5) M NO to a similar extent to LY 83583 alone, while the relaxant response to 10(-4) M GTN was reduced to the same extent as after 1 h exposure to 5.5 x 10(-4) M GTN alone.
7 It is concluded that S-nitrosothiols potently relax the rat gastric fundus, possibly by a cyclic GMP-dependent mechanism and S-nitrosothiols such as SNAC and GSNO may be involved in NANC neurotransmission.},
  author       = {Barbier, Ann and Lefebvre, Romain},
  issn         = {0007-1188},
  journal      = {BRITISH JOURNAL OF PHARMACOLOGY},
  keyword      = {ORGANIC NITRATES,ENDOTHELIUM,NONCHOLINERGIC RELAXATION,RELAXING FACTOR,NITRIC-OXIDE,VASCULAR SMOOTH-MUSCLE,NANC NERVE-STIMULATION,VASOACTIVE INTESTINAL POLYPEPTIDE,NITRATE TOLERANCE,NONADRENERGIC NONCHOLINERGIC (NANC) NERVES,S-NITROSOTHIOLS,RAT GASTRIC FUNDUS,MECHANISM,RELEASE},
  language     = {eng},
  number       = {4},
  pages        = {1280--1286},
  title        = {Influence of S-nitrosothiols and nitrate tolerance in the rat gastric fundus},
  url          = {http://dx.doi.org/10.1111/j.1476-5381.1994.tb14884.x},
  volume       = {111},
  year         = {1994},
}

Chicago
Barbier, Ann J, and Romain Lefebvre. 1994. “Influence of S-nitrosothiols and Nitrate Tolerance in the Rat Gastric Fundus.” British Journal of Pharmacology 111 (4): 1280–1286.
APA
Barbier, A. J., & Lefebvre, R. (1994). Influence of S-nitrosothiols and nitrate tolerance in the rat gastric fundus. BRITISH JOURNAL OF PHARMACOLOGY, 111(4), 1280–1286.
Vancouver
1.
Barbier AJ, Lefebvre R. Influence of S-nitrosothiols and nitrate tolerance in the rat gastric fundus. BRITISH JOURNAL OF PHARMACOLOGY. 1994;111(4):1280–6.
MLA
Barbier, Ann J, and Romain Lefebvre. “Influence of S-nitrosothiols and Nitrate Tolerance in the Rat Gastric Fundus.” BRITISH JOURNAL OF PHARMACOLOGY 111.4 (1994): 1280–1286. Print.