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Birth of double-muscled Belgian Blue calves after transfer of in vitro produced embryos into dairy cattle

(1994) THERIOGENOLOGY. 41(4). p.855-867
Author
Organization
Abstract
The possible application of the bovine in vitro fertilization technique for economical beef production was evaluated by transferring in vitro produced Belgian Blue embryos to synchronized dairy cows and heifers. In total, 4167 oocytes, collected in the slaughterhouse from double-muscled Belgian Blue cows, were matured in vitro. Frozen-thawed semen from 3 Belgian Blue bulls was used for in vitro fertilization. Zygotes were cultured in B-2 + 10% estrous cow serum together with oviductal cells at 39 degrees C in 5% CO2 in air. After 7 days, 576 (13.8%) transferable embryos were obtained. One hundred and eighteen of the most advanced embryos were selected for Fresh transfer into 90 recipients. Some of the remaining embryos were frozen using conventional methods. After fresh transfer, 50 recipients (55.6%) had elevated progesterone at day 23. Thirty cows (33.3%) calved after a mean gestation length of 282.8 +/- 6.0 days and produced 25 single births and 5 twins. The sea ratio was 71.4%. The mean birth weight was 45.1 +/- 8.3 kg. Three calves were of the conventional type instead of double-muscled and 2 calves died of congenital malformations. After transfer of in vitro produced frozen-thawed Belgian Blue embryos into 27 recipients (1 embryo/recipient), 2 bull calves (7.4%) were born. Bovine embryo production by in vitro techniques could form a low-cost supply of beef calves. However, to render it commercially attractive, selection of sires and darns has to be performed with great care.
Keywords
IN VITRO, CATTLE, EMBRYO PRODUCTION, EMBRYO TRANSFER, SEX RATIO, INVITRO-FERTILIZED EMBRYOS, BOVINE EMBRYOS, TRANSFER PREGNANCIES, CUMULUS CELLS, OOCYTES, CULTURE, SEX, BLASTOCYSTS, CLEAVAGE, WHITE

Citation

Please use this url to cite or link to this publication:

MLA
Van Soom, Ann, Peter Mijten, I Van Vlaenderen, et al. “Birth of Double-muscled Belgian Blue Calves After Transfer of in Vitro Produced Embryos into Dairy Cattle.” THERIOGENOLOGY 41.4 (1994): 855–867. Print.
APA
Van Soom, A., Mijten, P., Van Vlaenderen, I., Van den Branden, J., Mahmoudzadeh, A., & de Kruif, A. (1994). Birth of double-muscled Belgian Blue calves after transfer of in vitro produced embryos into dairy cattle. THERIOGENOLOGY, 41(4), 855–867.
Chicago author-date
Van Soom, Ann, Peter Mijten, I Van Vlaenderen, J Van den Branden, AR Mahmoudzadeh, and Aart de Kruif. 1994. “Birth of Double-muscled Belgian Blue Calves After Transfer of in Vitro Produced Embryos into Dairy Cattle.” Theriogenology 41 (4): 855–867.
Chicago author-date (all authors)
Van Soom, Ann, Peter Mijten, I Van Vlaenderen, J Van den Branden, AR Mahmoudzadeh, and Aart de Kruif. 1994. “Birth of Double-muscled Belgian Blue Calves After Transfer of in Vitro Produced Embryos into Dairy Cattle.” Theriogenology 41 (4): 855–867.
Vancouver
1.
Van Soom A, Mijten P, Van Vlaenderen I, Van den Branden J, Mahmoudzadeh A, de Kruif A. Birth of double-muscled Belgian Blue calves after transfer of in vitro produced embryos into dairy cattle. THERIOGENOLOGY. 1994;41(4):855–67.
IEEE
[1]
A. Van Soom, P. Mijten, I. Van Vlaenderen, J. Van den Branden, A. Mahmoudzadeh, and A. de Kruif, “Birth of double-muscled Belgian Blue calves after transfer of in vitro produced embryos into dairy cattle,” THERIOGENOLOGY, vol. 41, no. 4, pp. 855–867, 1994.
@article{197104,
  abstract     = {The possible application of the bovine in vitro fertilization technique for economical beef production was evaluated by transferring in vitro produced Belgian Blue embryos to synchronized dairy cows and heifers. In total, 4167 oocytes, collected in the slaughterhouse from double-muscled Belgian Blue cows, were matured in vitro. Frozen-thawed semen from 3 Belgian Blue bulls was used for in vitro fertilization. Zygotes were cultured in B-2 + 10% estrous cow serum together with oviductal cells at 39 degrees C in 5% CO2 in air. After 7 days, 576 (13.8%) transferable embryos were obtained. One hundred and eighteen of the most advanced embryos were selected for Fresh transfer into 90 recipients. Some of the remaining embryos were frozen using conventional methods.
After fresh transfer, 50 recipients (55.6%) had elevated progesterone at day 23. Thirty cows (33.3%) calved after a mean gestation length of 282.8 +/- 6.0 days and produced 25 single births and 5 twins. The sea ratio was 71.4%. The mean birth weight was 45.1 +/- 8.3 kg. Three calves were of the conventional type instead of double-muscled and 2 calves died of congenital malformations.
After transfer of in vitro produced frozen-thawed Belgian Blue embryos into 27 recipients (1 embryo/recipient), 2 bull calves (7.4%) were born.
Bovine embryo production by in vitro techniques could form a low-cost supply of beef calves. However, to render it commercially attractive, selection of sires and darns has to be performed with great care.},
  author       = {Van Soom, Ann and Mijten, Peter and Van Vlaenderen, I and Van den Branden, J and Mahmoudzadeh, AR and de Kruif, Aart},
  issn         = {0093-691X},
  journal      = {THERIOGENOLOGY},
  keywords     = {IN VITRO,CATTLE,EMBRYO PRODUCTION,EMBRYO TRANSFER,SEX RATIO,INVITRO-FERTILIZED EMBRYOS,BOVINE EMBRYOS,TRANSFER PREGNANCIES,CUMULUS CELLS,OOCYTES,CULTURE,SEX,BLASTOCYSTS,CLEAVAGE,WHITE},
  language     = {eng},
  number       = {4},
  pages        = {855--867},
  title        = {Birth of double-muscled Belgian Blue calves after transfer of in vitro produced embryos into dairy cattle},
  url          = {http://dx.doi.org/10.1016/0093-691X(94)90501-9},
  volume       = {41},
  year         = {1994},
}

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