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Dissecting the role of CHITINASE-LIKE1 in nitrate-dependent changes in root architecture

Christian Hermans, Silvana Porco, Filip Vandenbussche UGent, Sascha Gille, Jerome De Pessemier, Dominique Van Der Straeten UGent, Nathalie Verbruggen and Daniel R Bush (2011) PLANT PHYSIOLOGY. 157(3). p.1313-1326
abstract
The root phenotype of an Arabidopsis (Arabidopsis thaliana) mutant of CHITINASE-LIKE1 (CTL1), called arm (for anion-related root morphology), was previously shown to be conditional on growth on high nitrate, chloride, or sucrose. Mutants grown under restrictive conditions displayed inhibition of primary root growth, radial swelling, proliferation of lateral roots, and increased root hair density. We found here that the spatial pattern of CTL1 expression was mainly in the root and root tips during seedling development and that the protein localized to the cell wall. Fourier-transform infrared microspectroscopy of mutant root tissues indicated differences in spectra assigned to linkages in cellulose and pectin. Indeed, root cell wall polymer composition analysis revealed that the arm mutant contained less crystalline cellulose and reduced methylesterification of pectins. We also explored the implication of growth regulators on the phenotype of the mutant response to the nitrate supply. Exogenous abscisic acid application inhibited more drastically primary root growth in the arm mutant but failed to repress lateral branching compared with the wild type. Cytokinin levels were higher in the arm root, but there were no changes in mitotic activity, suggesting that cytokinin is not directly involved in the mutant phenotype. Ethylene production was higher in arm but inversely proportional to the nitrate concentration in the medium. Interestingly, eto2 and eto3 ethylene overproduction mutants mimicked some of the conditional root characteristics of the arm mutant on high nitrate. Our data suggest that ethylene may be involved in the arm mutant phenotype, albeit indirectly, rather than functioning as a primary signal.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ARABIDOPSIS-THALIANA, CELL-WALL BIOSYNTHESIS, ABSCISIC-ACID, SYSTEM ARCHITECTURE, 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID, CELLULOSE SYNTHESIS, ECTOPIC DEPOSITION, MERISTEM ACTIVITY, RESPONSE PATHWAY, GENETIC-ANALYSIS
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
157
issue
3
pages
1313 - 1326
Web of Science type
Article
Web of Science id
000296722300027
JCR category
PLANT SCIENCES
JCR impact factor
6.535 (2011)
JCR rank
7/189 (2011)
JCR quartile
1 (2011)
ISSN
0032-0889
DOI
10.1104/pp.111.181461
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1969634
handle
http://hdl.handle.net/1854/LU-1969634
date created
2011-12-19 11:12:42
date last changed
2011-12-20 11:22:40
@article{1969634,
  abstract     = {The root phenotype of an Arabidopsis (Arabidopsis thaliana) mutant of CHITINASE-LIKE1 (CTL1), called arm (for anion-related root morphology), was previously shown to be conditional on growth on high nitrate, chloride, or sucrose. Mutants grown under restrictive conditions displayed inhibition of primary root growth, radial swelling, proliferation of lateral roots, and increased root hair density. We found here that the spatial pattern of CTL1 expression was mainly in the root and root tips during seedling development and that the protein localized to the cell wall. Fourier-transform infrared microspectroscopy of mutant root tissues indicated differences in spectra assigned to linkages in cellulose and pectin. Indeed, root cell wall polymer composition analysis revealed that the arm mutant contained less crystalline cellulose and reduced methylesterification of pectins. We also explored the implication of growth regulators on the phenotype of the mutant response to the nitrate supply. Exogenous abscisic acid application inhibited more drastically primary root growth in the arm mutant but failed to repress lateral branching compared with the wild type. Cytokinin levels were higher in the arm root, but there were no changes in mitotic activity, suggesting that cytokinin is not directly involved in the mutant phenotype. Ethylene production was higher in arm but inversely proportional to the nitrate concentration in the medium. Interestingly, eto2 and eto3 ethylene overproduction mutants mimicked some of the conditional root characteristics of the arm mutant on high nitrate. Our data suggest that ethylene may be involved in the arm mutant phenotype, albeit indirectly, rather than functioning as a primary signal.},
  author       = {Hermans, Christian and Porco, Silvana and Vandenbussche, Filip and Gille, Sascha and De Pessemier, Jerome and Van Der Straeten, Dominique and Verbruggen, Nathalie and Bush, Daniel R},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {ARABIDOPSIS-THALIANA,CELL-WALL BIOSYNTHESIS,ABSCISIC-ACID,SYSTEM ARCHITECTURE,1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID,CELLULOSE SYNTHESIS,ECTOPIC DEPOSITION,MERISTEM ACTIVITY,RESPONSE PATHWAY,GENETIC-ANALYSIS},
  language     = {eng},
  number       = {3},
  pages        = {1313--1326},
  title        = {Dissecting the role of CHITINASE-LIKE1 in nitrate-dependent changes in root architecture},
  url          = {http://dx.doi.org/10.1104/pp.111.181461},
  volume       = {157},
  year         = {2011},
}

Chicago
Hermans, Christian, Silvana Porco, Filip Vandenbussche, Sascha Gille, Jerome De Pessemier, Dominique Van Der Straeten, Nathalie Verbruggen, and Daniel R Bush. 2011. “Dissecting the Role of CHITINASE-LIKE1 in Nitrate-dependent Changes in Root Architecture.” Plant Physiology 157 (3): 1313–1326.
APA
Hermans, Christian, Porco, S., Vandenbussche, F., Gille, S., De Pessemier, J., Van Der Straeten, D., Verbruggen, N., et al. (2011). Dissecting the role of CHITINASE-LIKE1 in nitrate-dependent changes in root architecture. PLANT PHYSIOLOGY, 157(3), 1313–1326.
Vancouver
1.
Hermans C, Porco S, Vandenbussche F, Gille S, De Pessemier J, Van Der Straeten D, et al. Dissecting the role of CHITINASE-LIKE1 in nitrate-dependent changes in root architecture. PLANT PHYSIOLOGY. 2011;157(3):1313–26.
MLA
Hermans, Christian, Silvana Porco, Filip Vandenbussche, et al. “Dissecting the Role of CHITINASE-LIKE1 in Nitrate-dependent Changes in Root Architecture.” PLANT PHYSIOLOGY 157.3 (2011): 1313–1326. Print.