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Experimental modification of vitellogenesis in Japanese quail by trypan blue in vivo

Katharina D'Herde UGent and Frank Roels (1993) REPRODUCTION NUTRITION DEVELOPMENT. 33(6). p.597-608
abstract
Ovaries of adult Japanese quails were exposed in vivo to the acid bisazo dye trypan blue (TB) which binds to plasma albumin, the plasma precursor of the yolk protein alpha-livetin. By a combination of fluorescence microscopy and electron microscopy alpha-livetin could be localized in the subdroplets of intermediary and yellow yolk spheres. Trypan blue alters vitellogenesis in the non-disc region of follicles in rapid growth in a reversible and dose-dependent way. Less yolk is produced over 24 h and its morphology is different when compared to controls. This yolk is similar to yolk of the germinal disc region where vitellogenesis is known to be inhibited physiologically. Several ultrastructural features of the germinal disc region are also found in the non-disc region of TB-exposed follicles. Our results suggest that the morphology of yellow yolk is linked to the rate of deposition. We propose that the inhibitory action of TB on vitellogenesis can be explained by a defective-receptor-ligand dissociation in endosomes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
RAPID GROWTH-PHASE, TRACER, INDUCED FLUORESCENCE, OVARIAN FOLLICLE, XENOPUS-LAEVIS, YOLK, TRANSPORT, OOCYTES, LOCALIZATION, SEGREGATION, LAYERS, RECEPTOR-MEDIATED ENDOCYTOSIS, QUAIL, VITELLOGENESIS, ALPHA-LIVETIN
journal title
REPRODUCTION NUTRITION DEVELOPMENT
Reprod. Nutr. Dev.
volume
33
issue
6
pages
597 - 608
Web of Science type
Article
ISSN
0926-5287
DOI
10.1051/rnd:19930611
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
196851
handle
http://hdl.handle.net/1854/LU-196851
date created
2004-01-14 13:42:00
date last changed
2016-12-19 15:38:31
@article{196851,
  abstract     = {Ovaries of adult Japanese quails were exposed in vivo to the acid bisazo dye trypan blue (TB) which binds to plasma albumin, the plasma precursor of the yolk protein alpha-livetin. By a combination of fluorescence microscopy and electron microscopy alpha-livetin could be localized in the subdroplets of intermediary and yellow yolk spheres. Trypan blue alters vitellogenesis in the non-disc region of follicles in rapid growth in a reversible and dose-dependent way. Less yolk is produced over 24 h and its morphology is different when compared to controls. This yolk is similar to yolk of the germinal disc region where vitellogenesis is known to be inhibited physiologically. Several ultrastructural features of the germinal disc region are also found in the non-disc region of TB-exposed follicles. Our results suggest that the morphology of yellow yolk is linked to the rate of deposition. We propose that the inhibitory action of TB on vitellogenesis can be explained by a defective-receptor-ligand dissociation in endosomes.},
  author       = {D'Herde, Katharina and Roels, Frank},
  issn         = {0926-5287},
  journal      = {REPRODUCTION NUTRITION DEVELOPMENT},
  keyword      = {RAPID GROWTH-PHASE,TRACER,INDUCED FLUORESCENCE,OVARIAN FOLLICLE,XENOPUS-LAEVIS,YOLK,TRANSPORT,OOCYTES,LOCALIZATION,SEGREGATION,LAYERS,RECEPTOR-MEDIATED ENDOCYTOSIS,QUAIL,VITELLOGENESIS,ALPHA-LIVETIN},
  language     = {eng},
  number       = {6},
  pages        = {597--608},
  title        = {Experimental modification of vitellogenesis in Japanese quail by trypan blue in vivo},
  url          = {http://dx.doi.org/10.1051/rnd:19930611},
  volume       = {33},
  year         = {1993},
}

Chicago
D’Herde, Katharina, and Frank Roels. 1993. “Experimental Modification of Vitellogenesis in Japanese Quail by Trypan Blue in Vivo.” Reproduction Nutrition Development 33 (6): 597–608.
APA
D’Herde, K., & Roels, F. (1993). Experimental modification of vitellogenesis in Japanese quail by trypan blue in vivo. REPRODUCTION NUTRITION DEVELOPMENT, 33(6), 597–608.
Vancouver
1.
D’Herde K, Roels F. Experimental modification of vitellogenesis in Japanese quail by trypan blue in vivo. REPRODUCTION NUTRITION DEVELOPMENT. 1993;33(6):597–608.
MLA
D’Herde, Katharina, and Frank Roels. “Experimental Modification of Vitellogenesis in Japanese Quail by Trypan Blue in Vivo.” REPRODUCTION NUTRITION DEVELOPMENT 33.6 (1993): 597–608. Print.