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Assessing dextran nanogels for intravenous small interfering RNA delivery

(2011)
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(UGent) , (UGent) and (UGent)
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Abstract
Among numerous delivery technologies currently being investigated for siRNA delivery, dextran nanogels were recently developed by Raemdonck and colleagues. These matrix systems have been shown to efficiently deliver siRNA in vitro but whether these nanogels are suited for in vivo siRNA delivery remains to be investigated. In this thesis, an overview of the current matrix systems for siRNA delivery is given in CHAPTER 1 by means of introduction. Because the first generation of dextran nanogels does not possess any moieties for surface modifications, a new type of nanogels had to be developed to enable polyethylene coating. In CHAPTER 2, a new type of dextran nanogels containing primary amine groups for surface modifications is developed and characterized. The chapter continues with the evaluation of various methods for decorating the nanogel surface with PEG and its effects on the cellular uptake, toxicity and transfection efficiency. In the context of intravenous application of dextran nanogels, the behavior of the nanogels in complex media such as human serum and plasma is investigated in CHAPTER 3. Additionally, the interactions between nanoparticles and blood cells are studied in CHAPTER 4. Based on the conclusions of chapter 4, the effects of nanoparticles on monocytes are investigated in further detail in CHAPTER 5. More specifically, acute toxicity, production of reactive oxygen species (ROS) and differentiation are tested. In CHAPTER 6, this thesis is finalized with several in vivo experiments exploring the biodistribution and the potential of dextran nanogels for intravenous delivery of siRNA.

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Please use this url to cite or link to this publication:

Chicago
Naeye, Broes. 2011. “Assessing Dextran Nanogels for Intravenous Small Interfering RNA Delivery”. Ghent, Belgium: Ghent University. Faculty of Pharmaceutical Sciences.
APA
Naeye, B. (2011). Assessing dextran nanogels for intravenous small interfering RNA delivery. Ghent University. Faculty of Pharmaceutical Sciences, Ghent, Belgium.
Vancouver
1.
Naeye B. Assessing dextran nanogels for intravenous small interfering RNA delivery. [Ghent, Belgium]: Ghent University. Faculty of Pharmaceutical Sciences; 2011.
MLA
Naeye, Broes. “Assessing Dextran Nanogels for Intravenous Small Interfering RNA Delivery.” 2011 : n. pag. Print.
@phdthesis{1959681,
  abstract     = {Among numerous delivery technologies currently being investigated for siRNA delivery, dextran nanogels were recently developed by Raemdonck and colleagues. These matrix systems have been shown to efficiently deliver siRNA in vitro but whether these nanogels are suited for in vivo siRNA delivery remains to be investigated. In this thesis, an overview of the current matrix systems for siRNA delivery is given in CHAPTER 1 by means of introduction. Because the first generation of dextran nanogels does not possess any moieties for surface modifications, a new type of nanogels had to be developed to enable polyethylene coating. In CHAPTER 2, a new type of dextran nanogels containing primary amine groups for surface modifications is developed and characterized. The chapter continues with the evaluation of various methods for decorating the nanogel surface with PEG and its effects on the cellular uptake, toxicity and transfection efficiency. In the context of intravenous application of dextran nanogels, the behavior of the nanogels in complex media such as human serum and plasma is investigated in CHAPTER 3. Additionally, the interactions between nanoparticles and blood cells are studied in CHAPTER 4. Based on the conclusions of chapter 4, the effects of nanoparticles on monocytes are investigated in further detail in CHAPTER 5. More specifically, acute toxicity, production of reactive oxygen species (ROS) and differentiation are tested. In CHAPTER 6, this thesis is finalized with several in vivo experiments exploring the biodistribution and the potential of dextran nanogels for intravenous delivery of siRNA.},
  author       = {Naeye, Broes},
  language     = {eng},
  pages        = {140},
  publisher    = {Ghent University. Faculty of Pharmaceutical Sciences},
  school       = {Ghent University},
  title        = {Assessing dextran nanogels for intravenous small interfering RNA delivery},
  year         = {2011},
}