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Arabidopsis thaliana NADPH oxidoreductase homologs confer tolerance of yeasts toward the thiol-oxidizing drug diamide

Elena Babiychuk (UGent) , Sergei Kushnir (UGent) , Enric Belles-Boix (UGent) , Marc Van Montagu (UGent) and Dirk Inzé (UGent)
(1995) JOURNAL OF BIOLOGICAL CHEMISTRY. 270(44). p.26224-26231
Author
Organization
Abstract
To isolate new plant genes involved in the defense against oxidative stress, an Arabidopsis cDNA library in a yeast expression vector was transformed into a yeast strain deficient in the YAP1 gene, which encodes a b-Zip transcription factor and regulates general stress response in yeasts. Cells from approximate to 10(5) primary transformants were subjected to a tolerance screen toward the thiol-oxidizing drug diamide, which depletes the reduced glutathione in the cell, Four types of Arobidopsis cDNAs were isolated, Three of these cDNAs (PI, Pa, and P4) belong to a plant zeta-crystallin family and P3 is an Arabidopsis homolog of isoflavonoid reductases. As such, all four isolated cDNAs are homologous to NADPH oxidoreductases. PI, P2, and PS steady-state mRNAs accumulated rapidly in Arabidopsis plants under various oxidative stress conditions, such as treatment with paraquat, t-butylhydroperoxide, diamide, and menadione. The data suggested that proteins encoded by the isolated cDNAs play a distinct role in plant antioxidant defense and are possibly involved in NAD(P)/NAD(P)H homeostasis.
Keywords
SACCHAROMYCES-CEREVISIAE GENE, ESCHERICHIA-COLI, DEPENDENT 15-HYDROXYPROSTAGLANDIN DEHYDROGENASE, GUINEA-PIG LENS, PHYTOALEXIN BIOSYNTHESIS, MOLECULAR-CLONING, ZETA-CRYSTALLIN, ASCORBATE PEROXIDASE, SUPEROXIDE-DISMUTASE GENE, STRESS RESPONSES

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MLA
Babiychuk, Elena, Sergei Kushnir, Enric Belles-Boix, et al. “Arabidopsis Thaliana NADPH Oxidoreductase Homologs Confer Tolerance of Yeasts Toward the Thiol-oxidizing Drug Diamide.” JOURNAL OF BIOLOGICAL CHEMISTRY 270.44 (1995): 26224–26231. Print.
APA
Babiychuk, E., Kushnir, S., Belles-Boix, E., Van Montagu, M., & Inzé, D. (1995). Arabidopsis thaliana NADPH oxidoreductase homologs confer tolerance of yeasts toward the thiol-oxidizing drug diamide. JOURNAL OF BIOLOGICAL CHEMISTRY, 270(44), 26224–26231.
Chicago author-date
Babiychuk, Elena, Sergei Kushnir, Enric Belles-Boix, Marc Van Montagu, and Dirk Inzé. 1995. “Arabidopsis Thaliana NADPH Oxidoreductase Homologs Confer Tolerance of Yeasts Toward the Thiol-oxidizing Drug Diamide.” Journal of Biological Chemistry 270 (44): 26224–26231.
Chicago author-date (all authors)
Babiychuk, Elena, Sergei Kushnir, Enric Belles-Boix, Marc Van Montagu, and Dirk Inzé. 1995. “Arabidopsis Thaliana NADPH Oxidoreductase Homologs Confer Tolerance of Yeasts Toward the Thiol-oxidizing Drug Diamide.” Journal of Biological Chemistry 270 (44): 26224–26231.
Vancouver
1.
Babiychuk E, Kushnir S, Belles-Boix E, Van Montagu M, Inzé D. Arabidopsis thaliana NADPH oxidoreductase homologs confer tolerance of yeasts toward the thiol-oxidizing drug diamide. JOURNAL OF BIOLOGICAL CHEMISTRY. 1995;270(44):26224–31.
IEEE
[1]
E. Babiychuk, S. Kushnir, E. Belles-Boix, M. Van Montagu, and D. Inzé, “Arabidopsis thaliana NADPH oxidoreductase homologs confer tolerance of yeasts toward the thiol-oxidizing drug diamide,” JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 270, no. 44, pp. 26224–26231, 1995.
@article{195929,
  abstract     = {To isolate new plant genes involved in the defense against oxidative stress, an Arabidopsis cDNA library in a yeast expression vector was transformed into a yeast strain deficient in the YAP1 gene, which encodes a b-Zip transcription factor and regulates general stress response in yeasts. Cells from approximate to 10(5) primary transformants were subjected to a tolerance screen toward the thiol-oxidizing drug diamide, which depletes the reduced glutathione in the cell, Four types of Arobidopsis cDNAs were isolated, Three of these cDNAs (PI, Pa, and P4) belong to a plant zeta-crystallin family and P3 is an Arabidopsis homolog of isoflavonoid reductases. As such, all four isolated cDNAs are homologous to NADPH oxidoreductases. PI, P2, and PS steady-state mRNAs accumulated rapidly in Arabidopsis plants under various oxidative stress conditions, such as treatment with paraquat, t-butylhydroperoxide, diamide, and menadione. The data suggested that proteins encoded by the isolated cDNAs play a distinct role in plant antioxidant defense and are possibly involved in NAD(P)/NAD(P)H homeostasis.},
  author       = {Babiychuk, Elena and Kushnir, Sergei and Belles-Boix, Enric and Van Montagu, Marc and Inzé, Dirk},
  issn         = {0021-9258},
  journal      = {JOURNAL OF BIOLOGICAL CHEMISTRY},
  keywords     = {SACCHAROMYCES-CEREVISIAE GENE,ESCHERICHIA-COLI,DEPENDENT 15-HYDROXYPROSTAGLANDIN DEHYDROGENASE,GUINEA-PIG LENS,PHYTOALEXIN BIOSYNTHESIS,MOLECULAR-CLONING,ZETA-CRYSTALLIN,ASCORBATE PEROXIDASE,SUPEROXIDE-DISMUTASE GENE,STRESS RESPONSES},
  language     = {eng},
  number       = {44},
  pages        = {26224--26231},
  title        = {Arabidopsis thaliana NADPH oxidoreductase homologs confer tolerance of yeasts toward the thiol-oxidizing drug diamide},
  url          = {http://dx.doi.org/10.1074/jbc.270.44.26224},
  volume       = {270},
  year         = {1995},
}

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