Ghent University Academic Bibliography

Advanced

The nodD locus from Azorhizobium caulinodans is flanked by two repetitive elements

Danny Geelen UGent, Koen Goethals UGent, Marc Van Montagu UGent and Marcella Holsters UGent (1995) GENE. 164(1). p.107-111
abstract
The sequence surrounding the Azorhizobium caulinodans (Ac) regulatory nodD gene was analyzed. Upstream from nodD and in the opposite orientation, two small open reading frames were identified (ORF1 and ORF2). The DNA sequence corresponding to ORF1, termed epsilon 1, is similar to a part of the insertion element IS51 from Pseudomonas savastanoi. Immediately downstream from nodD, a repeated element, delta 1, has been described [Goethals et al., Mel. Plant-Microbe Interact. 5 (1992) 405-411]. The elements epsilon 1 and delta 1 form the borders of a shift in GC content between nodD and its surrounding sequences. delta 1 and the ORF1+ORF2 sequence both occur as two copies in the Ac genome. Based on these observations, we postulate that the repeated elements played a role in the horizontal transfer of nodD during evolution. Insertion mutations in epsilon 1 and delta 1 did not influence the induction of the nodulation operon, nodABCSUIJ, and had no effect on the nodulation behavior on Sesbania rostrata. lacZ fusion studies suggested that nodD is constitutively transcribed and that the promoter driving nodD expression overlaps with the ORF1 sequence. In contrast, promoter activity in the direction of ORF1 and ORF2 was not observed. In the nodD-ORF1-intervening sequence, a nod box-related motif was recognized that deviates from active nod boxes by the absence of an ATC-9-bp-GAT palindrome, i.e., a sequence involved in NodD-mediated transcription stimulation [Goethals et al., Proc. Natl. Acad. Sci. USA 89 (1992) 1646-1650].
Please use this url to cite or link to this publication:
author
organization
alternative title
The nodD locus from Azorhizobium caulinodans is flanked by 2 repetitive elements
year
type
journalArticle (original)
publication status
published
subject
keyword
INSERTION SEQUENCE, GENOME, NOD BOX, NODULATION, RHIZOBIUM, NODULATION GENES, DNA-SEQUENCES, HOST RANGE, AGROBACTERIUM-TUMEFACIENS, NUCLEOTIDE-SEQUENCE, IDENTIFICATION, ORS571, PROMOTERS, PROTEINS
journal title
GENE
Gene
volume
164
issue
1
pages
107 - 111
Web of Science type
Article
ISSN
0378-1119
DOI
10.1016/0378-1119(95)00456-G
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
195830
handle
http://hdl.handle.net/1854/LU-195830
date created
2004-01-14 13:42:00
date last changed
2011-10-07 14:57:36
@article{195830,
  abstract     = {The sequence surrounding the Azorhizobium caulinodans (Ac) regulatory nodD gene was analyzed. Upstream from nodD and in the opposite orientation, two small open reading frames were identified (ORF1 and ORF2). The DNA sequence corresponding to ORF1, termed epsilon 1, is similar to a part of the insertion element IS51 from Pseudomonas savastanoi. Immediately downstream from nodD, a repeated element, delta 1, has been described [Goethals et al., Mel. Plant-Microbe Interact. 5 (1992) 405-411]. The elements epsilon 1 and delta 1 form the borders of a shift in GC content between nodD and its surrounding sequences. delta 1 and the ORF1+ORF2 sequence both occur as two copies in the Ac genome. Based on these observations, we postulate that the repeated elements played a role in the horizontal transfer of nodD during evolution. Insertion mutations in epsilon 1 and delta 1 did not influence the induction of the nodulation operon, nodABCSUIJ, and had no effect on the nodulation behavior on Sesbania rostrata. lacZ fusion studies suggested that nodD is constitutively transcribed and that the promoter driving nodD expression overlaps with the ORF1 sequence. In contrast, promoter activity in the direction of ORF1 and ORF2 was not observed. In the nodD-ORF1-intervening sequence, a nod box-related motif was recognized that deviates from active nod boxes by the absence of an ATC-9-bp-GAT palindrome, i.e., a sequence involved in NodD-mediated transcription stimulation [Goethals et al., Proc. Natl. Acad. Sci. USA 89 (1992) 1646-1650].},
  author       = {Geelen, Danny and Goethals, Koen and Van Montagu, Marc and Holsters, Marcella},
  issn         = {0378-1119},
  journal      = {GENE},
  keyword      = {INSERTION SEQUENCE,GENOME,NOD BOX,NODULATION,RHIZOBIUM,NODULATION GENES,DNA-SEQUENCES,HOST RANGE,AGROBACTERIUM-TUMEFACIENS,NUCLEOTIDE-SEQUENCE,IDENTIFICATION,ORS571,PROMOTERS,PROTEINS},
  language     = {eng},
  number       = {1},
  pages        = {107--111},
  title        = {The nodD locus from Azorhizobium caulinodans is flanked by two repetitive elements},
  url          = {http://dx.doi.org/10.1016/0378-1119(95)00456-G},
  volume       = {164},
  year         = {1995},
}

Chicago
Geelen, Danny, Koen Goethals, Marc Van Montagu, and Marcella Holsters. 1995. “The nodD Locus from Azorhizobium Caulinodans Is Flanked by Two Repetitive Elements.” Gene 164 (1): 107–111.
APA
Geelen, D., Goethals, K., Van Montagu, M., & Holsters, M. (1995). The nodD locus from Azorhizobium caulinodans is flanked by two repetitive elements. GENE, 164(1), 107–111.
Vancouver
1.
Geelen D, Goethals K, Van Montagu M, Holsters M. The nodD locus from Azorhizobium caulinodans is flanked by two repetitive elements. GENE. 1995;164(1):107–11.
MLA
Geelen, Danny, Koen Goethals, Marc Van Montagu, et al. “The nodD Locus from Azorhizobium Caulinodans Is Flanked by Two Repetitive Elements.” GENE 164.1 (1995): 107–111. Print.