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Varying effects of different β-glucans on the maturation of porcine monocyte-derived dendritic cells

Eva Sonck (UGent) , Bert Devriendt (UGent) , Bruno Goddeeris (UGent) and Eric Cox (UGent)
Author
Organization
Abstract
Beta-glucans are well known for their immunomodulatory capacities in human and mice. For this reason together with the European ban on growth-promoting antibiotics, b-glucans are intensively used in pig feed. However, as described in the present study, there is much variation in the stimulatory capacity between b-glucans of different sources. As dendritic cells (DCs) are the first cells which are encountered after an antigen is taken up by the intestinal epithelial cell barrier, we decided to investigate the effect of two concentrations (5 and 10 µg/ml) of five commercial b-glucanpreparations, differing in structure and source, on porcine monocyte-derived dendritic cells (MoDCs). Although all b-glucans gave rise to a significant reduction of the phagocytic activity of DCs, only Macrogard induced a significant phenotypical maturation. Besides Macrogard, also zymosan, another b-glucan derived from Saccharomyces cerevisiae, and curdlan significantly improved the T cell stimulatory capacity of MoDCs. Most interesting however, is the cytokine secretion profile of curdlan-stimulated MoDCs as only curdlan induced significant higher expression levels of IL-1b, IL-6, IL-10 and IL-12/IL-23p40. As the cytokine profile of DCs influences the outcome of the ensuing immune response and thus may prove valuable in intestinal immunity, a careful choice is necessary when b-glucans are used as dietary supplement.
Keywords
dendritic cells, pigs, beta-glucans

Citation

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Chicago
Sonck, Eva, Bert Devriendt, Bruno Goddeeris, and Eric Cox. 2011. “Varying Effects of Different Β-glucans on the Maturation of Porcine Monocyte-derived Dendritic Cells.” In EMDS, 25th Annual Meeting, Abstracts. European Macrophage and Dendritic cell Society (EMDS).
APA
Sonck, E., Devriendt, B., Goddeeris, B., & Cox, E. (2011). Varying effects of different β-glucans on the maturation of porcine monocyte-derived dendritic cells. EMDS, 25th Annual meeting, Abstracts. Presented at the 25th Annual EMDS meeting : Clinical and fundamental aspects of monocyte, macrophage and DC plasticity, European Macrophage and Dendritic cell Society (EMDS).
Vancouver
1.
Sonck E, Devriendt B, Goddeeris B, Cox E. Varying effects of different β-glucans on the maturation of porcine monocyte-derived dendritic cells. EMDS, 25th Annual meeting, Abstracts. European Macrophage and Dendritic cell Society (EMDS); 2011.
MLA
Sonck, Eva, Bert Devriendt, Bruno Goddeeris, et al. “Varying Effects of Different Β-glucans on the Maturation of Porcine Monocyte-derived Dendritic Cells.” EMDS, 25th Annual Meeting, Abstracts. European Macrophage and Dendritic cell Society (EMDS), 2011. Print.
@inproceedings{1957058,
  abstract     = {Beta-glucans are well known for their immunomodulatory capacities in human and mice. For this reason together with the European ban on growth-promoting antibiotics, b-glucans are intensively used in pig feed. However, as described in the present study, there is much variation in the stimulatory capacity between b-glucans of different sources. As dendritic cells (DCs) are the first cells which are encountered after an antigen is taken up by the intestinal epithelial cell barrier, we decided to investigate the effect of two concentrations (5 and 10 {\textmu}g/ml) of five commercial b-glucanpreparations, differing in structure and source, on porcine monocyte-derived dendritic cells (MoDCs). Although all b-glucans gave rise to a significant reduction of the phagocytic activity of DCs, only Macrogard induced a significant phenotypical maturation. Besides Macrogard, also zymosan, another b-glucan derived from Saccharomyces cerevisiae, and curdlan significantly improved the T cell stimulatory capacity of MoDCs. Most interesting however, is the cytokine secretion profile of curdlan-stimulated MoDCs as only curdlan induced significant higher expression levels of IL-1b, IL-6, IL-10 and IL-12/IL-23p40. As the cytokine profile of DCs influences the outcome of the ensuing immune response and thus may prove valuable in intestinal immunity, a careful choice is necessary when b-glucans are used as dietary supplement.},
  author       = {Sonck, Eva and Devriendt, Bert and Goddeeris, Bruno and Cox, Eric},
  booktitle    = {EMDS, 25th Annual meeting, Abstracts},
  keyword      = {dendritic cells,pigs,beta-glucans},
  language     = {eng},
  location     = {Brussels, Belgium},
  publisher    = {European Macrophage and Dendritic cell Society (EMDS)},
  title        = {Varying effects of different \ensuremath{\beta}-glucans on the maturation of porcine monocyte-derived dendritic cells},
  year         = {2011},
}