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Development of a 3D pigmented skin model to evaluate RNAi-induced depigmentation

Mireille Van Gele UGent, Barbara Geusens UGent, Reinhart Speeckaert UGent, Peter Dynoodt UGent, Barbara Vanhoecke UGent, Karolien Van Den Bossche UGent and Jo Lambert UGent (2011) EXPERIMENTAL DERMATOLOGY. 20(9). p.773-775
abstract
Because current skin whitening agents often have insufficient efficacy and side effects, we aim to develop effective and safe therapeutics using RNA interference (RNAi). We established a pigmented human-reconstructed skin model as a first step in the development of novel siRNA-based depigmenting agents. Histological characterization revealed that our model had a similar morphology as normal human skin, expressed keratinocyte differentiation as well as basement membrane markers, and showed a high degree of pigmentation. The utility of the model to study RNAi-induced depigmentation was validated by incorporation of melanocytes transfected with siRNA against tyrosinase, a key enzyme in skin pigmentation. This resulted in a strong reduction in pigmentation and inhibition of melanin transfer proving that siRNA-mediated gene silencing in melanocytes worked successfully in our model. Therefore, this self-made 3D skin model will be a useful and easy tool to validate the whitening potential of candidate genes with a presumed function in melanin synthesis or transfer.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
depigmentation, melanocytes, reconstructed skin, tyrosinase, RNA interference, MELANOCYTES, KNOCKDOWN
journal title
EXPERIMENTAL DERMATOLOGY
Exp. Dermatol.
volume
20
issue
9
pages
773 - 775
Web of Science type
Article
Web of Science id
000294594000019
JCR category
DERMATOLOGY
JCR impact factor
3.543 (2011)
JCR rank
8/58 (2011)
JCR quartile
1 (2011)
ISSN
0906-6705
DOI
10.1111/j.1600-0625.2011.01319.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1955750
handle
http://hdl.handle.net/1854/LU-1955750
date created
2011-11-29 13:49:12
date last changed
2014-06-20 14:27:10
@article{1955750,
  abstract     = {Because current skin whitening agents often have insufficient efficacy and side effects, we aim to develop effective and safe therapeutics using RNA interference (RNAi). We established a pigmented human-reconstructed skin model as a first step in the development of novel siRNA-based depigmenting agents. Histological characterization revealed that our model had a similar morphology as normal human skin, expressed keratinocyte differentiation as well as basement membrane markers, and showed a high degree of pigmentation. The utility of the model to study RNAi-induced depigmentation was validated by incorporation of melanocytes transfected with siRNA against tyrosinase, a key enzyme in skin pigmentation. This resulted in a strong reduction in pigmentation and inhibition of melanin transfer proving that siRNA-mediated gene silencing in melanocytes worked successfully in our model. Therefore, this self-made 3D skin model will be a useful and easy tool to validate the whitening potential of candidate genes with a presumed function in melanin synthesis or transfer.},
  author       = {Van Gele, Mireille and Geusens, Barbara and Speeckaert, Reinhart and Dynoodt, Peter and Vanhoecke, Barbara and Van Den Bossche, Karolien and Lambert, Jo},
  issn         = {0906-6705},
  journal      = {EXPERIMENTAL DERMATOLOGY},
  keyword      = {depigmentation,melanocytes,reconstructed skin,tyrosinase,RNA interference,MELANOCYTES,KNOCKDOWN},
  language     = {eng},
  number       = {9},
  pages        = {773--775},
  title        = {Development of a 3D pigmented skin model to evaluate RNAi-induced depigmentation},
  url          = {http://dx.doi.org/10.1111/j.1600-0625.2011.01319.x},
  volume       = {20},
  year         = {2011},
}

Chicago
Van Gele, Mireille, Barbara Geusens, Reinhart Speeckaert, Peter Dynoodt, Barbara Vanhoecke, Karolien Van Den Bossche, and Jo Lambert. 2011. “Development of a 3D Pigmented Skin Model to Evaluate RNAi-induced Depigmentation.” Experimental Dermatology 20 (9): 773–775.
APA
Van Gele, M., Geusens, B., Speeckaert, R., Dynoodt, P., Vanhoecke, B., Van Den Bossche, K., & Lambert, J. (2011). Development of a 3D pigmented skin model to evaluate RNAi-induced depigmentation. EXPERIMENTAL DERMATOLOGY, 20(9), 773–775.
Vancouver
1.
Van Gele M, Geusens B, Speeckaert R, Dynoodt P, Vanhoecke B, Van Den Bossche K, et al. Development of a 3D pigmented skin model to evaluate RNAi-induced depigmentation. EXPERIMENTAL DERMATOLOGY. 2011;20(9):773–5.
MLA
Van Gele, Mireille, Barbara Geusens, Reinhart Speeckaert, et al. “Development of a 3D Pigmented Skin Model to Evaluate RNAi-induced Depigmentation.” EXPERIMENTAL DERMATOLOGY 20.9 (2011): 773–775. Print.