Development of a 3D pigmented skin model to evaluate RNAi-induced depigmentation
- Author
- Mireille Van Gele (UGent) , Barbara Geusens (UGent) , Reinhart Speeckaert (UGent) , Peter Dynoodt (UGent) , Barbara Vanhoecke (UGent) , Karolien Van Den Bossche (UGent) and Jo Lambert (UGent)
- Organization
- Abstract
- Because current skin whitening agents often have insufficient efficacy and side effects, we aim to develop effective and safe therapeutics using RNA interference (RNAi). We established a pigmented human-reconstructed skin model as a first step in the development of novel siRNA-based depigmenting agents. Histological characterization revealed that our model had a similar morphology as normal human skin, expressed keratinocyte differentiation as well as basement membrane markers, and showed a high degree of pigmentation. The utility of the model to study RNAi-induced depigmentation was validated by incorporation of melanocytes transfected with siRNA against tyrosinase, a key enzyme in skin pigmentation. This resulted in a strong reduction in pigmentation and inhibition of melanin transfer proving that siRNA-mediated gene silencing in melanocytes worked successfully in our model. Therefore, this self-made 3D skin model will be a useful and easy tool to validate the whitening potential of candidate genes with a presumed function in melanin synthesis or transfer.
- Keywords
- depigmentation, melanocytes, reconstructed skin, tyrosinase, RNA interference, MELANOCYTES, KNOCKDOWN
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-1955750
- MLA
- Van Gele, Mireille, et al. “Development of a 3D Pigmented Skin Model to Evaluate RNAi-Induced Depigmentation.” EXPERIMENTAL DERMATOLOGY, vol. 20, no. 9, 2011, pp. 773–75, doi:10.1111/j.1600-0625.2011.01319.x.
- APA
- Van Gele, M., Geusens, B., Speeckaert, R., Dynoodt, P., Vanhoecke, B., Van Den Bossche, K., & Lambert, J. (2011). Development of a 3D pigmented skin model to evaluate RNAi-induced depigmentation. EXPERIMENTAL DERMATOLOGY, 20(9), 773–775. https://doi.org/10.1111/j.1600-0625.2011.01319.x
- Chicago author-date
- Van Gele, Mireille, Barbara Geusens, Reinhart Speeckaert, Peter Dynoodt, Barbara Vanhoecke, Karolien Van Den Bossche, and Jo Lambert. 2011. “Development of a 3D Pigmented Skin Model to Evaluate RNAi-Induced Depigmentation.” EXPERIMENTAL DERMATOLOGY 20 (9): 773–75. https://doi.org/10.1111/j.1600-0625.2011.01319.x.
- Chicago author-date (all authors)
- Van Gele, Mireille, Barbara Geusens, Reinhart Speeckaert, Peter Dynoodt, Barbara Vanhoecke, Karolien Van Den Bossche, and Jo Lambert. 2011. “Development of a 3D Pigmented Skin Model to Evaluate RNAi-Induced Depigmentation.” EXPERIMENTAL DERMATOLOGY 20 (9): 773–775. doi:10.1111/j.1600-0625.2011.01319.x.
- Vancouver
- 1.Van Gele M, Geusens B, Speeckaert R, Dynoodt P, Vanhoecke B, Van Den Bossche K, et al. Development of a 3D pigmented skin model to evaluate RNAi-induced depigmentation. EXPERIMENTAL DERMATOLOGY. 2011;20(9):773–5.
- IEEE
- [1]M. Van Gele et al., “Development of a 3D pigmented skin model to evaluate RNAi-induced depigmentation,” EXPERIMENTAL DERMATOLOGY, vol. 20, no. 9, pp. 773–775, 2011.
@article{1955750,
abstract = {{Because current skin whitening agents often have insufficient efficacy and side effects, we aim to develop effective and safe therapeutics using RNA interference (RNAi). We established a pigmented human-reconstructed skin model as a first step in the development of novel siRNA-based depigmenting agents. Histological characterization revealed that our model had a similar morphology as normal human skin, expressed keratinocyte differentiation as well as basement membrane markers, and showed a high degree of pigmentation. The utility of the model to study RNAi-induced depigmentation was validated by incorporation of melanocytes transfected with siRNA against tyrosinase, a key enzyme in skin pigmentation. This resulted in a strong reduction in pigmentation and inhibition of melanin transfer proving that siRNA-mediated gene silencing in melanocytes worked successfully in our model. Therefore, this self-made 3D skin model will be a useful and easy tool to validate the whitening potential of candidate genes with a presumed function in melanin synthesis or transfer.}},
author = {{Van Gele, Mireille and Geusens, Barbara and Speeckaert, Reinhart and Dynoodt, Peter and Vanhoecke, Barbara and Van Den Bossche, Karolien and Lambert, Jo}},
issn = {{0906-6705}},
journal = {{EXPERIMENTAL DERMATOLOGY}},
keywords = {{depigmentation,melanocytes,reconstructed skin,tyrosinase,RNA interference,MELANOCYTES,KNOCKDOWN}},
language = {{eng}},
number = {{9}},
pages = {{773--775}},
title = {{Development of a 3D pigmented skin model to evaluate RNAi-induced depigmentation}},
url = {{http://doi.org/10.1111/j.1600-0625.2011.01319.x}},
volume = {{20}},
year = {{2011}},
}
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