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Development of a 3D pigmented skin model to evaluate RNAi-induced depigmentation

Mireille Van Gele UGent, Barbara Geusens UGent, Reinhart Speeckaert, Peter Dynoodt UGent, Barbara Vanhoecke, Karolien Van Den Bossche UGent and Jo Lambert UGent (2011) EXPERIMENTAL DERMATOLOGY. 20(9). p.773-775
abstract
Because current skin whitening agents often have insufficient efficacy and side effects, we aim to develop effective and safe therapeutics using RNA interference (RNAi). We established a pigmented human-reconstructed skin model as a first step in the development of novel siRNA-based depigmenting agents. Histological characterization revealed that our model had a similar morphology as normal human skin, expressed keratinocyte differentiation as well as basement membrane markers, and showed a high degree of pigmentation. The utility of the model to study RNAi-induced depigmentation was validated by incorporation of melanocytes transfected with siRNA against tyrosinase, a key enzyme in skin pigmentation. This resulted in a strong reduction in pigmentation and inhibition of melanin transfer proving that siRNA-mediated gene silencing in melanocytes worked successfully in our model. Therefore, this self-made 3D skin model will be a useful and easy tool to validate the whitening potential of candidate genes with a presumed function in melanin synthesis or transfer.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
depigmentation, melanocytes, reconstructed skin, tyrosinase, RNA interference, MELANOCYTES, KNOCKDOWN
journal title
EXPERIMENTAL DERMATOLOGY
Exp. Dermatol.
volume
20
issue
9
pages
773 - 775
Web of Science type
Article
Web of Science id
000294594000019
JCR category
DERMATOLOGY
JCR impact factor
3.543 (2011)
JCR rank
8/58 (2011)
JCR quartile
1 (2011)
ISSN
0906-6705
DOI
10.1111/j.1600-0625.2011.01319.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1955750
handle
http://hdl.handle.net/1854/LU-1955750
date created
2011-11-29 13:49:12
date last changed
2016-12-19 15:39:50
@article{1955750,
  abstract     = {Because current skin whitening agents often have insufficient efficacy and side effects, we aim to develop effective and safe therapeutics using RNA interference (RNAi). We established a pigmented human-reconstructed skin model as a first step in the development of novel siRNA-based depigmenting agents. Histological characterization revealed that our model had a similar morphology as normal human skin, expressed keratinocyte differentiation as well as basement membrane markers, and showed a high degree of pigmentation. The utility of the model to study RNAi-induced depigmentation was validated by incorporation of melanocytes transfected with siRNA against tyrosinase, a key enzyme in skin pigmentation. This resulted in a strong reduction in pigmentation and inhibition of melanin transfer proving that siRNA-mediated gene silencing in melanocytes worked successfully in our model. Therefore, this self-made 3D skin model will be a useful and easy tool to validate the whitening potential of candidate genes with a presumed function in melanin synthesis or transfer.},
  author       = {Van Gele, Mireille and Geusens, Barbara and Speeckaert, Reinhart and Dynoodt, Peter and Vanhoecke, Barbara and Van Den Bossche, Karolien and Lambert, Jo},
  issn         = {0906-6705},
  journal      = {EXPERIMENTAL DERMATOLOGY},
  keyword      = {depigmentation,melanocytes,reconstructed skin,tyrosinase,RNA interference,MELANOCYTES,KNOCKDOWN},
  language     = {eng},
  number       = {9},
  pages        = {773--775},
  title        = {Development of a 3D pigmented skin model to evaluate RNAi-induced depigmentation},
  url          = {http://dx.doi.org/10.1111/j.1600-0625.2011.01319.x},
  volume       = {20},
  year         = {2011},
}

Chicago
Van Gele, Mireille, Barbara Geusens, Reinhart Speeckaert, Peter Dynoodt, Barbara Vanhoecke, Karolien Van Den Bossche, and Jo Lambert. 2011. “Development of a 3D Pigmented Skin Model to Evaluate RNAi-induced Depigmentation.” Experimental Dermatology 20 (9): 773–775.
APA
Van Gele, M., Geusens, B., Speeckaert, R., Dynoodt, P., Vanhoecke, B., Van Den Bossche, K., & Lambert, J. (2011). Development of a 3D pigmented skin model to evaluate RNAi-induced depigmentation. EXPERIMENTAL DERMATOLOGY, 20(9), 773–775.
Vancouver
1.
Van Gele M, Geusens B, Speeckaert R, Dynoodt P, Vanhoecke B, Van Den Bossche K, et al. Development of a 3D pigmented skin model to evaluate RNAi-induced depigmentation. EXPERIMENTAL DERMATOLOGY. 2011;20(9):773–5.
MLA
Van Gele, Mireille, Barbara Geusens, Reinhart Speeckaert, et al. “Development of a 3D Pigmented Skin Model to Evaluate RNAi-induced Depigmentation.” EXPERIMENTAL DERMATOLOGY 20.9 (2011): 773–775. Print.