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Immunochemically unreactive albumin in urine: fiction or reality?

Marijn Speeckaert UGent, Reinhart Speeckaert UGent, LIEN VAN DE VOORDE UGent and Joris Delanghe UGent (2011) CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES. 48(2). p.87-96
abstract
Urinary albumin measurements are currently not standardized due to a lack of a reference method and reference (primary and secondary [matrix]) material. Multiple molecular forms of albumin in urine are identified. Modification of albumin by proteolysis during passage through the urinary tract and chemical modification during specimen storage leads to the formation of albumin fragments. Multiple methods have been developed to quantify albuminuria and significant different results are reported dependent on the available assay. The current point of view of the National Kidney Disease Education Program - IFCC Working Group on Standardization of Albumin considers the immunoassay with polyclonal sera as the primary method of quantifying urine albumin. This article reviews the process of albumin fragmentation and focuses on the controversial topic of immuno-unreactive, nonimmunoreactive, or immunochemically nonreactive albumin fractions and its consequences for albumin analysis. We conclude that at present there are no hard arguments for measuring immunochemically unreactive albumin in urine. Immunoassays using polyclonal antisera for the detection of urinary albumin remain the gold standard. The development of a reference measurement procedure remains one of the challenges for the future.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (review)
publication status
published
subject
keyword
assays, Albumin fragmentation, immunochemically unreactive albumin, LYSOSOMAL ACTIVITY, CARDIOVASCULAR-DISEASE, SIZE-EXCLUSION HPLC, PROXIMAL TUBULE CELLS, INFLAMMATORY-BOWEL-DISEASE, STREPTOZOTOCIN-DIABETIC RATS, PROTEIN-KINASE-C, MASS-SPECTROMETRY, HUMAN-SERUM-ALBUMIN, PERFORMANCE LIQUID-CHROMATOGRAPHY
journal title
CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES
Crit. Rev. Clin. Lab. Sci.
volume
48
issue
2
pages
87 - 96
Web of Science type
Review
Web of Science id
000294492000003
JCR category
MEDICAL LABORATORY TECHNOLOGY
JCR impact factor
5.25 (2011)
JCR rank
2/32 (2011)
JCR quartile
1 (2011)
ISSN
1040-8363
DOI
10.3109/10408363.2011.591366
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1955001
handle
http://hdl.handle.net/1854/LU-1955001
date created
2011-11-28 15:12:29
date last changed
2011-12-02 16:54:42
@article{1955001,
  abstract     = {Urinary albumin measurements are currently not standardized due to a lack of a reference method and reference (primary and secondary [matrix]) material. Multiple molecular forms of albumin in urine are identified. Modification of albumin by proteolysis during passage through the urinary tract and chemical modification during specimen storage leads to the formation of albumin fragments. Multiple methods have been developed to quantify albuminuria and significant different results are reported dependent on the available assay. The current point of view of the National Kidney Disease Education Program - IFCC Working Group on Standardization of Albumin considers the immunoassay with polyclonal sera as the primary method of quantifying urine albumin. This article reviews the process of albumin fragmentation and focuses on the controversial topic of immuno-unreactive, nonimmunoreactive, or immunochemically nonreactive albumin fractions and its consequences for albumin analysis. We conclude that at present there are no hard arguments for measuring immunochemically unreactive albumin in urine. Immunoassays using polyclonal antisera for the detection of urinary albumin remain the gold standard. The development of a reference measurement procedure remains one of the challenges for the future.},
  author       = {Speeckaert, Marijn and Speeckaert, Reinhart and VAN DE VOORDE, LIEN and Delanghe, Joris},
  issn         = {1040-8363},
  journal      = {CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES},
  keyword      = {assays,Albumin fragmentation,immunochemically unreactive albumin,LYSOSOMAL ACTIVITY,CARDIOVASCULAR-DISEASE,SIZE-EXCLUSION HPLC,PROXIMAL TUBULE CELLS,INFLAMMATORY-BOWEL-DISEASE,STREPTOZOTOCIN-DIABETIC RATS,PROTEIN-KINASE-C,MASS-SPECTROMETRY,HUMAN-SERUM-ALBUMIN,PERFORMANCE LIQUID-CHROMATOGRAPHY},
  language     = {eng},
  number       = {2},
  pages        = {87--96},
  title        = {Immunochemically unreactive albumin in urine: fiction or reality?},
  url          = {http://dx.doi.org/10.3109/10408363.2011.591366},
  volume       = {48},
  year         = {2011},
}

Chicago
SPEECKAERT, MARIJN, Reinhart Speeckaert, LIEN VAN DE VOORDE, and Joris Delanghe. 2011. “Immunochemically Unreactive Albumin in Urine: Fiction or Reality?” Critical Reviews in Clinical Laboratory Sciences 48 (2): 87–96.
APA
SPEECKAERT, M., Speeckaert, R., VAN DE VOORDE, L., & Delanghe, J. (2011). Immunochemically unreactive albumin in urine: fiction or reality? CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES, 48(2), 87–96.
Vancouver
1.
SPEECKAERT M, Speeckaert R, VAN DE VOORDE L, Delanghe J. Immunochemically unreactive albumin in urine: fiction or reality? CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES. 2011;48(2):87–96.
MLA
SPEECKAERT, MARIJN, Reinhart Speeckaert, LIEN VAN DE VOORDE, et al. “Immunochemically Unreactive Albumin in Urine: Fiction or Reality?” CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES 48.2 (2011): 87–96. Print.