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Phosphorylation of a mitotic kinesin-like protein and a MAPKKK by cyclin-dependent kinases (CDKs) is involved in the transition to cytokinesis in plants

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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Cytokinesis in eukaryotes involves specific arrays of microtubules (MTs), which are known as the "central spindle" in animals, the "anaphase spindle" in yeasts, and the "phragmoplast" in plants. Control of these arrays, which are composed mainly of bundled nonkinetochore MTs, is critically important during cytokinesis. In plants, an MAPK cascade stimulates the turnover of phragmoplast MTs, and a crucial aspect of the activation of this cascade is the interaction between the MAPKKK, nucleus-and phragmoplast-localized protein kinase 1 (NPK1) and the NPK1-activating kinesin-like protein 1 (NACK1), a key regulator of plant cytokinesis. However, little is known about the control of this interaction at the molecular level during progression through the M phase. We demonstrated that cyclin-dependent kinases (CDKs) phosphorylate both NPK1 and NACK1 before metaphase in tobacco cells, thereby inhibiting the interaction between these proteins, suggesting that such phosphorylation prevents the transition to cytokinesis. Failure to inactivate CDKs after metaphase prevents dephosphorylation of these two proteins, causing incomplete mitosis. Experiments with Arabidopsis NACK1 (AtNACK1/HINKEL) revealed that phosphorylated NACK1 fails to mediate cytokinesis. Thus, timely and coordinated phosphorylation by CDKs and dephosphorylation of cytokinetic regulators from prophase to anaphase appear to be critical for the appropriate onset and/or progression of cytokinesis.
Keywords
ANAPHASE, PRC1, BINDING, TRANSLOCATION, FISSION, ARABIDOPSIS-THALIANA, MIDZONE FORMATION, MICROTUBULE DYNAMICS, MAP65, CELL-PLATE, SPINDLE ELONGATION, phospho-mimic, cell plate, cell cycle

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Citation

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Chicago
Sasabe, Michiko, Véronique Boudolf, Lieven De Veylder, Dirk Inzé, Pascal Genschik, and Yasunori Machida. 2011. “Phosphorylation of a Mitotic Kinesin-like Protein and a MAPKKK by Cyclin-dependent Kinases (CDKs) Is Involved in the Transition to Cytokinesis in Plants.” Proceedings of the National Academy of Sciences of the United States of America 108 (43): 17844–17849.
APA
Sasabe, M., Boudolf, V., De Veylder, L., Inzé, D., Genschik, P., & Machida, Y. (2011). Phosphorylation of a mitotic kinesin-like protein and a MAPKKK by cyclin-dependent kinases (CDKs) is involved in the transition to cytokinesis in plants. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 108(43), 17844–17849.
Vancouver
1.
Sasabe M, Boudolf V, De Veylder L, Inzé D, Genschik P, Machida Y. Phosphorylation of a mitotic kinesin-like protein and a MAPKKK by cyclin-dependent kinases (CDKs) is involved in the transition to cytokinesis in plants. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2011;108(43):17844–9.
MLA
Sasabe, Michiko, Véronique Boudolf, Lieven De Veylder, et al. “Phosphorylation of a Mitotic Kinesin-like Protein and a MAPKKK by Cyclin-dependent Kinases (CDKs) Is Involved in the Transition to Cytokinesis in Plants.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 108.43 (2011): 17844–17849. Print.
@article{1954393,
  abstract     = {Cytokinesis in eukaryotes involves specific arrays of microtubules (MTs), which are known as the {\textacutedbl}central spindle{\textacutedbl} in animals, the {\textacutedbl}anaphase spindle{\textacutedbl} in yeasts, and the {\textacutedbl}phragmoplast{\textacutedbl} in plants. Control of these arrays, which are composed mainly of bundled nonkinetochore MTs, is critically important during cytokinesis. In plants, an MAPK cascade stimulates the turnover of phragmoplast MTs, and a crucial aspect of the activation of this cascade is the interaction between the MAPKKK, nucleus-and phragmoplast-localized protein kinase 1 (NPK1) and the NPK1-activating kinesin-like protein 1 (NACK1), a key regulator of plant cytokinesis. However, little is known about the control of this interaction at the molecular level during progression through the M phase. We demonstrated that cyclin-dependent kinases (CDKs) phosphorylate both NPK1 and NACK1 before metaphase in tobacco cells, thereby inhibiting the interaction between these proteins, suggesting that such phosphorylation prevents the transition to cytokinesis. Failure to inactivate CDKs after metaphase prevents dephosphorylation of these two proteins, causing incomplete mitosis. Experiments with Arabidopsis NACK1 (AtNACK1/HINKEL) revealed that phosphorylated NACK1 fails to mediate cytokinesis. Thus, timely and coordinated phosphorylation by CDKs and dephosphorylation of cytokinetic regulators from prophase to anaphase appear to be critical for the appropriate onset and/or progression of cytokinesis.},
  author       = {Sasabe, Michiko and Boudolf, V{\'e}ronique and De Veylder, Lieven and Inz{\'e}, Dirk and Genschik, Pascal and Machida, Yasunori},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  language     = {eng},
  number       = {43},
  pages        = {17844--17849},
  title        = {Phosphorylation of a mitotic kinesin-like protein and a MAPKKK by cyclin-dependent kinases (CDKs) is involved in the transition to cytokinesis in plants},
  url          = {http://dx.doi.org/10.1073/pnas.1110174108},
  volume       = {108},
  year         = {2011},
}

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