Ghent University Academic Bibliography

Advanced

Recycling, clustering, and endocytosis jointly maintain PIN auxin carrier polarity at the plasma membrane

Jürgen Kleine-Vehn UGent, Krzysztof Wabnik UGent, Alexandre Martinière, Lukasz Langowski UGent, Katrin Willig, Satoshi Naramoto UGent, Johannes Leitner, Hirokazu Tanaka UGent, Stefan Jakobs and Stephanie Robert UGent, et al. (2011) MOLECULAR SYSTEMS BIOLOGY. 7.
abstract
Cell polarity reflected by asymmetric distribution of proteins at the plasma membrane is a fundamental feature of unicellular and multicellular organisms. It remains conceptually unclear how cell polarity is kept in cell wall-encapsulated plant cells. We have used super-resolution and semi-quantitative live-cell imaging in combination with pharmacological, genetic, and computational approaches to reveal insights into the mechanism of cell polarity maintenance in Arabidopsis thaliana. We show that polar-competent PIN transporters for the phytohormone auxin are delivered to the center of polar domains by super-polar recycling. Within the plasma membrane, PINs are recruited into non-mobile membrane clusters and their lateral diffusion is dramatically reduced, which ensures longer polar retention. At the circumventing edges of the polar domain, spatially defined internalization of escaped cargos occurs by clathrin-dependent endocytosis. Computer simulations confirm that the combination of these processes provides a robust mechanism for polarity maintenance in plant cells. Moreover, our study suggests that the regulation of lateral diffusion and spatially defined endocytosis, but not super-polar exocytosis have primary importance for PIN polarity maintenance.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
TRAFFICKING, CLATHRIN-DEPENDENT ENDOCYTOSIS, PROTEINS, TRANSPORT, DIFFERENTIATION, EFFLUX, ARABIDOPSIS, CELL POLARITY, STIMULATED-EMISSION, FEEDBACK-REGULATION, cell polarity, Stimulated Emission Depletion microscopy (STED), membrane trafficking, modeling, systems biology, trafficking
journal title
MOLECULAR SYSTEMS BIOLOGY
Mol. Syst. Biol.
volume
7
article_number
540
pages
13 pages
Web of Science type
Article
Web of Science id
000296652600004
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
8.626 (2011)
JCR rank
24/286 (2011)
JCR quartile
1 (2011)
ISSN
1744-4292
DOI
10.1038/msb.2011.72
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1954372
handle
http://hdl.handle.net/1854/LU-1954372
date created
2011-11-25 16:52:05
date last changed
2011-11-29 09:48:18
@article{1954372,
  abstract     = {Cell polarity reflected by asymmetric distribution of proteins at the plasma membrane is a fundamental feature of unicellular and multicellular organisms. It remains conceptually unclear how cell polarity is kept in cell wall-encapsulated plant cells. We have used super-resolution and semi-quantitative live-cell imaging in combination with pharmacological, genetic, and computational approaches to reveal insights into the mechanism of cell polarity maintenance in Arabidopsis thaliana. We show that polar-competent PIN transporters for the phytohormone auxin are delivered to the center of polar domains by super-polar recycling. Within the plasma membrane, PINs are recruited into non-mobile membrane clusters and their lateral diffusion is dramatically reduced, which ensures longer polar retention. At the circumventing edges of the polar domain, spatially defined internalization of escaped cargos occurs by clathrin-dependent endocytosis. Computer simulations confirm that the combination of these processes provides a robust mechanism for polarity maintenance in plant cells. Moreover, our study suggests that the regulation of lateral diffusion and spatially defined endocytosis, but not super-polar exocytosis have primary importance for PIN polarity maintenance.},
  articleno    = {540},
  author       = {Kleine-Vehn, J{\"u}rgen and Wabnik, Krzysztof and Martini{\`e}re, Alexandre and Langowski, Lukasz and Willig, Katrin and Naramoto, Satoshi and Leitner, Johannes and Tanaka, Hirokazu and Jakobs, Stefan and Robert, Stephanie and Luschnig, Christian and Govaerts, Willy and Hell, Stefan W and Runions, John and Friml, Jiri},
  issn         = {1744-4292},
  journal      = {MOLECULAR SYSTEMS BIOLOGY},
  keyword      = {TRAFFICKING,CLATHRIN-DEPENDENT ENDOCYTOSIS,PROTEINS,TRANSPORT,DIFFERENTIATION,EFFLUX,ARABIDOPSIS,CELL POLARITY,STIMULATED-EMISSION,FEEDBACK-REGULATION,cell polarity,Stimulated Emission Depletion microscopy (STED),membrane trafficking,modeling,systems biology,trafficking},
  language     = {eng},
  pages        = {13},
  title        = {Recycling, clustering, and endocytosis jointly maintain PIN auxin carrier polarity at the plasma membrane},
  url          = {http://dx.doi.org/10.1038/msb.2011.72},
  volume       = {7},
  year         = {2011},
}

Chicago
Kleine-Vehn, Jürgen, Krzysztof Wabnik, Alexandre Martinière, Lukasz Langowski, Katrin Willig, Satoshi Naramoto, Johannes Leitner, et al. 2011. “Recycling, Clustering, and Endocytosis Jointly Maintain PIN Auxin Carrier Polarity at the Plasma Membrane.” Molecular Systems Biology 7.
APA
Kleine-Vehn, J., Wabnik, K., Martinière, A., Langowski, L., Willig, K., Naramoto, S., Leitner, J., et al. (2011). Recycling, clustering, and endocytosis jointly maintain PIN auxin carrier polarity at the plasma membrane. MOLECULAR SYSTEMS BIOLOGY, 7.
Vancouver
1.
Kleine-Vehn J, Wabnik K, Martinière A, Langowski L, Willig K, Naramoto S, et al. Recycling, clustering, and endocytosis jointly maintain PIN auxin carrier polarity at the plasma membrane. MOLECULAR SYSTEMS BIOLOGY. 2011;7.
MLA
Kleine-Vehn, Jürgen, Krzysztof Wabnik, Alexandre Martinière, et al. “Recycling, Clustering, and Endocytosis Jointly Maintain PIN Auxin Carrier Polarity at the Plasma Membrane.” MOLECULAR SYSTEMS BIOLOGY 7 (2011): n. pag. Print.