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Recycling, clustering, and endocytosis jointly maintain PIN auxin carrier polarity at the plasma membrane

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Abstract
Cell polarity reflected by asymmetric distribution of proteins at the plasma membrane is a fundamental feature of unicellular and multicellular organisms. It remains conceptually unclear how cell polarity is kept in cell wall-encapsulated plant cells. We have used super-resolution and semi-quantitative live-cell imaging in combination with pharmacological, genetic, and computational approaches to reveal insights into the mechanism of cell polarity maintenance in Arabidopsis thaliana. We show that polar-competent PIN transporters for the phytohormone auxin are delivered to the center of polar domains by super-polar recycling. Within the plasma membrane, PINs are recruited into non-mobile membrane clusters and their lateral diffusion is dramatically reduced, which ensures longer polar retention. At the circumventing edges of the polar domain, spatially defined internalization of escaped cargos occurs by clathrin-dependent endocytosis. Computer simulations confirm that the combination of these processes provides a robust mechanism for polarity maintenance in plant cells. Moreover, our study suggests that the regulation of lateral diffusion and spatially defined endocytosis, but not super-polar exocytosis have primary importance for PIN polarity maintenance.
Keywords
TRAFFICKING, CLATHRIN-DEPENDENT ENDOCYTOSIS, PROTEINS, TRANSPORT, DIFFERENTIATION, EFFLUX, ARABIDOPSIS, CELL POLARITY, STIMULATED-EMISSION, FEEDBACK-REGULATION, cell polarity, Stimulated Emission Depletion microscopy (STED), membrane trafficking, modeling, systems biology, trafficking

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Chicago
Kleine-Vehn, Jürgen, Krzysztof Wabnik, Alexandre Martinière, Lukasz Langowski, Katrin Willig, Satoshi Naramoto, Johannes Leitner, et al. 2011. “Recycling, Clustering, and Endocytosis Jointly Maintain PIN Auxin Carrier Polarity at the Plasma Membrane.” Molecular Systems Biology 7.
APA
Kleine-Vehn, J., Wabnik, K., Martinière, A., Langowski, L., Willig, K., Naramoto, S., Leitner, J., et al. (2011). Recycling, clustering, and endocytosis jointly maintain PIN auxin carrier polarity at the plasma membrane. MOLECULAR SYSTEMS BIOLOGY, 7.
Vancouver
1.
Kleine-Vehn J, Wabnik K, Martinière A, Langowski L, Willig K, Naramoto S, et al. Recycling, clustering, and endocytosis jointly maintain PIN auxin carrier polarity at the plasma membrane. MOLECULAR SYSTEMS BIOLOGY. 2011;7.
MLA
Kleine-Vehn, Jürgen, Krzysztof Wabnik, Alexandre Martinière, et al. “Recycling, Clustering, and Endocytosis Jointly Maintain PIN Auxin Carrier Polarity at the Plasma Membrane.” MOLECULAR SYSTEMS BIOLOGY 7 (2011): n. pag. Print.
@article{1954372,
  abstract     = {Cell polarity reflected by asymmetric distribution of proteins at the plasma membrane is a fundamental feature of unicellular and multicellular organisms. It remains conceptually unclear how cell polarity is kept in cell wall-encapsulated plant cells. We have used super-resolution and semi-quantitative live-cell imaging in combination with pharmacological, genetic, and computational approaches to reveal insights into the mechanism of cell polarity maintenance in Arabidopsis thaliana. We show that polar-competent PIN transporters for the phytohormone auxin are delivered to the center of polar domains by super-polar recycling. Within the plasma membrane, PINs are recruited into non-mobile membrane clusters and their lateral diffusion is dramatically reduced, which ensures longer polar retention. At the circumventing edges of the polar domain, spatially defined internalization of escaped cargos occurs by clathrin-dependent endocytosis. Computer simulations confirm that the combination of these processes provides a robust mechanism for polarity maintenance in plant cells. Moreover, our study suggests that the regulation of lateral diffusion and spatially defined endocytosis, but not super-polar exocytosis have primary importance for PIN polarity maintenance.},
  articleno    = {540},
  author       = {Kleine-Vehn, J{\"u}rgen and Wabnik, Krzysztof and Martini{\`e}re, Alexandre and Langowski, Lukasz and Willig, Katrin and Naramoto, Satoshi and Leitner, Johannes and Tanaka, Hirokazu and Jakobs, Stefan and Robert, Stephanie and Luschnig, Christian and Govaerts, Willy and Hell, Stefan W and Runions, John and Friml, Jiri},
  issn         = {1744-4292},
  journal      = {MOLECULAR SYSTEMS BIOLOGY},
  keyword      = {TRAFFICKING,CLATHRIN-DEPENDENT ENDOCYTOSIS,PROTEINS,TRANSPORT,DIFFERENTIATION,EFFLUX,ARABIDOPSIS,CELL POLARITY,STIMULATED-EMISSION,FEEDBACK-REGULATION,cell polarity,Stimulated Emission Depletion microscopy (STED),membrane trafficking,modeling,systems biology,trafficking},
  language     = {eng},
  pages        = {13},
  title        = {Recycling, clustering, and endocytosis jointly maintain PIN auxin carrier polarity at the plasma membrane},
  url          = {http://dx.doi.org/10.1038/msb.2011.72},
  volume       = {7},
  year         = {2011},
}

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