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Isolation, characterization, and chromosomal location of a gene encoding the Δ¹-pyrroline-5-carboxylate synthetase in Arabidopsis thaliana

(1995) FEBS LETTERS. 372(1). p.13-19
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Organization
Abstract
A full-length cDNA and the corresponding At-PSS gene encoding the first enzyme of the proline biosynthetic pathway, the Delta(1)-pyrroline-5-carboxylate (P5C) synthetase, mere isolated in Arabidopsis thaliana. The At-P5S cDNA encodes a protein of 717 amino acids showing high identity with the P5C synthetase of Vigna aconitifolia. Strong homology is also found at the N-terminus to bacterial and yeast gamma-glutamyl kinase and at the C-terminus to bacterial gamma-glutamyl phosphate reductase, Putative ATP- and NAD(P)H-binding sites are suggested in the At-PSS protein, The transcribed region of the At-PSS gene is 4.8 kb long and contains 20 exons, Southern analysis suggests the presence of only one At-PSS gene in the A, thaliana genome mapped at the bottom of the chromosome two. Expression analysis of At-P5S in different organs reveals abundant At-PSS transcripts in mature flowering plant, Rapid induction of the Af-PSS gene followed by accumulation of proline was observed in NaCl-treated seedlings suggesting that At-P5S is osmoregulated.
Keywords
PROTEIN SECONDARY STRUCTURE, SALT STRESS, PROLINE BIOSYNTHESIS, ESCHERICHIA-COLI, SACCHAROMYCES-CEREVISIAE, OSMOTIC-STRESS, OSMOREGULATION, PREDICTION, SEQUENCE, PLANTS, RESPONSES, ARABIDOPSIS THALIANA, PROLINE, PYRROLINE-5-CARBOXYLATE SYNTHETASE

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MLA
Savouré, Arnould, Samir Jaoua, Xuejun Hua, et al. “Isolation, Characterization, and Chromosomal Location of a Gene Encoding the Δ1-pyrroline-5-carboxylate Synthetase in Arabidopsis Thaliana.” FEBS LETTERS 372.1 (1995): 13–19. Print.
APA
Savouré, A., Jaoua, S., Hua, X., Ardiles Diaz, W., Van Montagu, M., & Verbruggen, N. (1995). Isolation, characterization, and chromosomal location of a gene encoding the Δ1-pyrroline-5-carboxylate synthetase in Arabidopsis thaliana. FEBS LETTERS, 372(1), 13–19.
Chicago author-date
Savouré, Arnould, Samir Jaoua, Xuejun Hua, Wilson Ardiles Diaz, Marc Van Montagu, and Nathalie Verbruggen. 1995. “Isolation, Characterization, and Chromosomal Location of a Gene Encoding the Δ1-pyrroline-5-carboxylate Synthetase in Arabidopsis Thaliana.” Febs Letters 372 (1): 13–19.
Chicago author-date (all authors)
Savouré, Arnould, Samir Jaoua, Xuejun Hua, Wilson Ardiles Diaz, Marc Van Montagu, and Nathalie Verbruggen. 1995. “Isolation, Characterization, and Chromosomal Location of a Gene Encoding the Δ1-pyrroline-5-carboxylate Synthetase in Arabidopsis Thaliana.” Febs Letters 372 (1): 13–19.
Vancouver
1.
Savouré A, Jaoua S, Hua X, Ardiles Diaz W, Van Montagu M, Verbruggen N. Isolation, characterization, and chromosomal location of a gene encoding the Δ1-pyrroline-5-carboxylate synthetase in Arabidopsis thaliana. FEBS LETTERS. 1995;372(1):13–9.
IEEE
[1]
A. Savouré, S. Jaoua, X. Hua, W. Ardiles Diaz, M. Van Montagu, and N. Verbruggen, “Isolation, characterization, and chromosomal location of a gene encoding the Δ1-pyrroline-5-carboxylate synthetase in Arabidopsis thaliana,” FEBS LETTERS, vol. 372, no. 1, pp. 13–19, 1995.
@article{195371,
  abstract     = {A full-length cDNA and the corresponding At-PSS gene encoding the first enzyme of the proline biosynthetic pathway, the Delta(1)-pyrroline-5-carboxylate (P5C) synthetase, mere isolated in Arabidopsis thaliana. The At-P5S cDNA encodes a protein of 717 amino acids showing high identity with the P5C synthetase of Vigna aconitifolia. Strong homology is also found at the N-terminus to bacterial and yeast gamma-glutamyl kinase and at the C-terminus to bacterial gamma-glutamyl phosphate reductase, Putative ATP- and NAD(P)H-binding sites are suggested in the At-PSS protein, The transcribed region of the At-PSS gene is 4.8 kb long and contains 20 exons, Southern analysis suggests the presence of only one At-PSS gene in the A, thaliana genome mapped at the bottom of the chromosome two. Expression analysis of At-P5S in different organs reveals abundant At-PSS transcripts in mature flowering plant, Rapid induction of the Af-PSS gene followed by accumulation of proline was observed in NaCl-treated seedlings suggesting that At-P5S is osmoregulated.},
  author       = {Savouré, Arnould and Jaoua, Samir and Hua, Xuejun and Ardiles Diaz, Wilson and Van Montagu, Marc and Verbruggen, Nathalie},
  issn         = {0014-5793},
  journal      = {FEBS LETTERS},
  keywords     = {PROTEIN SECONDARY STRUCTURE,SALT STRESS,PROLINE BIOSYNTHESIS,ESCHERICHIA-COLI,SACCHAROMYCES-CEREVISIAE,OSMOTIC-STRESS,OSMOREGULATION,PREDICTION,SEQUENCE,PLANTS,RESPONSES,ARABIDOPSIS THALIANA,PROLINE,PYRROLINE-5-CARBOXYLATE SYNTHETASE},
  language     = {eng},
  number       = {1},
  pages        = {13--19},
  title        = {Isolation, characterization, and chromosomal location of a gene encoding the Δ¹-pyrroline-5-carboxylate synthetase in Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1016/0014-5793(95)00935-3},
  volume       = {372},
  year         = {1995},
}

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