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A method for testing drought tolerance in Fragaria based on fast screening for water deficit response and use of associated AFLP and EST candidate gene markers

(2011) EUPHYTICA. 180(3). p.385-409
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Abstract
Drought stress is one of the most important environmental factors that limit plant growth and development, thus reducing yield. The objective of the present research was to correlate the genetic structure of different Fragaria genotypes, as assessed by Expressed Sequence Tag (EST) and Amplified Fragment Length Polymorphism (AFLP) markers, and plant responses to drought stress. Firstly, physiological parameters related to the plant response to drought stress such as leaf relative water content (RWC) and water losing rate (WLR) were measured. WLR and RWC were compared for 20 cultivars of the octaploid Fragaria x ananassa, two ecotypes of the diploid species F. vesca and one octaploid species F. chiloensis. These parameters could discriminate genotypes showing a contrasting response to water stress. Secondly, AFLP and ESTs were compared in terms of their information content and efficiency in the study of genetic diversity and relationships among these 23 Fragaria genotypes. To evaluate the genetic basis for the observed variation in the measured physiological parameter, the effect of specific AFLP/EST loci on WLR and RWC for the different Fragaria genotypes was quantified by Kruskal-Wallis analysis. By Mantel testing, the hierarchical clustering of the Fragaria genotypes based on associated EST or AFLP markers was compared to the observed eco-physiological relevant grouping. A better discriminating capacity for associated markers was noted, enabling a functional marker selection approach to screen the strawberry gene pool for drought tolerance. Correlation of EST markers to leaf RWC and WLR enforces them as potential candidate genes in control of plant responses to drought stress in Fragaria sp.
Keywords
Genetic relationship, Clustering analysis, AFLP markers, EST marker, Water deficit, Drought stress, Strawberry cultivar, Fragaria vesca, Fragaria chiloensis, Fragaria x ananassa

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Chicago
Razavi Hosseinabadi, Farzaneh, Ellen De Keyser, Jan De Riek, and Marie-Christine Van Labeke. 2011. “A Method for Testing Drought Tolerance in Fragaria Based on Fast Screening for Water Deficit Response and Use of Associated AFLP and EST Candidate Gene Markers.” Euphytica 180 (3): 385–409.
APA
Razavi Hosseinabadi, F., De Keyser, E., De Riek, J., & Van Labeke, M.-C. (2011). A method for testing drought tolerance in Fragaria based on fast screening for water deficit response and use of associated AFLP and EST candidate gene markers. EUPHYTICA, 180(3), 385–409.
Vancouver
1.
Razavi Hosseinabadi F, De Keyser E, De Riek J, Van Labeke M-C. A method for testing drought tolerance in Fragaria based on fast screening for water deficit response and use of associated AFLP and EST candidate gene markers. EUPHYTICA. 2011;180(3):385–409.
MLA
Razavi Hosseinabadi, Farzaneh, Ellen De Keyser, Jan De Riek, et al. “A Method for Testing Drought Tolerance in Fragaria Based on Fast Screening for Water Deficit Response and Use of Associated AFLP and EST Candidate Gene Markers.” EUPHYTICA 180.3 (2011): 385–409. Print.
@article{1949149,
  abstract     = {Drought stress is one of the most important environmental factors that limit plant growth and development, thus reducing yield. The objective of the present research was to correlate the genetic structure of different Fragaria genotypes, as assessed by Expressed Sequence Tag (EST) and Amplified Fragment Length Polymorphism (AFLP) markers, and plant responses to drought stress. Firstly, physiological parameters related to the plant response to drought stress such as leaf relative water content (RWC) and water losing rate (WLR) were measured. WLR and RWC were compared for 20 cultivars of the octaploid Fragaria x ananassa, two ecotypes of the diploid species F. vesca and one octaploid species F. chiloensis. These parameters could discriminate genotypes showing a contrasting response to water stress. Secondly, AFLP and ESTs were compared in terms of their information content and efficiency in the study of genetic diversity and relationships among these 23 Fragaria genotypes. To evaluate the genetic basis for the observed variation in the measured physiological parameter, the effect of specific AFLP/EST loci on WLR and RWC for the different Fragaria genotypes was quantified by Kruskal-Wallis analysis. By Mantel testing, the hierarchical clustering of the Fragaria genotypes based on associated EST or AFLP markers was compared to the observed eco-physiological relevant grouping. A better discriminating capacity for associated markers was noted, enabling a functional marker selection approach to screen the strawberry gene pool for drought tolerance. Correlation of EST markers to leaf RWC and WLR enforces them as potential candidate genes in control of plant responses to drought stress in Fragaria sp.},
  author       = {Razavi Hosseinabadi, Farzaneh and De Keyser, Ellen and De Riek, Jan and Van Labeke, Marie-Christine},
  issn         = {0014-2336},
  journal      = {EUPHYTICA},
  keyword      = {Genetic relationship,Clustering analysis,AFLP markers,EST marker,Water deficit,Drought stress,Strawberry cultivar,Fragaria vesca,Fragaria chiloensis,Fragaria x ananassa},
  language     = {eng},
  number       = {3},
  pages        = {385--409},
  title        = {A method for testing drought tolerance in Fragaria based on fast screening for water deficit response and use of associated AFLP and EST candidate gene markers},
  url          = {http://dx.doi.org/10.1007/s10681-011-0398-x},
  volume       = {180},
  year         = {2011},
}

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