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A method for testing drought tolerance in Fragaria based on fast screening for water deficit response and use of associated AFLP and EST candidate gene markers

Farzaneh Razavi Hosseinabadi UGent, Ellen De Keyser UGent, Jan De Riek and Marie-Christine Van Labeke UGent (2011) EUPHYTICA. 180(3). p.385-409
abstract
Drought stress is one of the most important environmental factors that limit plant growth and development, thus reducing yield. The objective of the present research was to correlate the genetic structure of different Fragaria genotypes, as assessed by Expressed Sequence Tag (EST) and Amplified Fragment Length Polymorphism (AFLP) markers, and plant responses to drought stress. Firstly, physiological parameters related to the plant response to drought stress such as leaf relative water content (RWC) and water losing rate (WLR) were measured. WLR and RWC were compared for 20 cultivars of the octaploid Fragaria x ananassa, two ecotypes of the diploid species F. vesca and one octaploid species F. chiloensis. These parameters could discriminate genotypes showing a contrasting response to water stress. Secondly, AFLP and ESTs were compared in terms of their information content and efficiency in the study of genetic diversity and relationships among these 23 Fragaria genotypes. To evaluate the genetic basis for the observed variation in the measured physiological parameter, the effect of specific AFLP/EST loci on WLR and RWC for the different Fragaria genotypes was quantified by Kruskal-Wallis analysis. By Mantel testing, the hierarchical clustering of the Fragaria genotypes based on associated EST or AFLP markers was compared to the observed eco-physiological relevant grouping. A better discriminating capacity for associated markers was noted, enabling a functional marker selection approach to screen the strawberry gene pool for drought tolerance. Correlation of EST markers to leaf RWC and WLR enforces them as potential candidate genes in control of plant responses to drought stress in Fragaria sp.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Genetic relationship, Clustering analysis, AFLP markers, EST marker, Water deficit, Drought stress, Strawberry cultivar, Fragaria vesca, Fragaria chiloensis, Fragaria x ananassa
journal title
EUPHYTICA
Euphytica
volume
180
issue
3
pages
385 - 409
Web of Science type
Article
Web of Science id
000291998600009
JCR category
HORTICULTURE
JCR impact factor
1.554 (2011)
JCR rank
7/31 (2011)
JCR quartile
1 (2011)
ISSN
0014-2336
DOI
10.1007/s10681-011-0398-x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1949149
handle
http://hdl.handle.net/1854/LU-1949149
date created
2011-11-24 19:49:24
date last changed
2011-11-28 08:39:41
@article{1949149,
  abstract     = {Drought stress is one of the most important environmental factors that limit plant growth and development, thus reducing yield. The objective of the present research was to correlate the genetic structure of different Fragaria genotypes, as assessed by Expressed Sequence Tag (EST) and Amplified Fragment Length Polymorphism (AFLP) markers, and plant responses to drought stress. Firstly, physiological parameters related to the plant response to drought stress such as leaf relative water content (RWC) and water losing rate (WLR) were measured. WLR and RWC were compared for 20 cultivars of the octaploid Fragaria x ananassa, two ecotypes of the diploid species F. vesca and one octaploid species F. chiloensis. These parameters could discriminate genotypes showing a contrasting response to water stress. Secondly, AFLP and ESTs were compared in terms of their information content and efficiency in the study of genetic diversity and relationships among these 23 Fragaria genotypes. To evaluate the genetic basis for the observed variation in the measured physiological parameter, the effect of specific AFLP/EST loci on WLR and RWC for the different Fragaria genotypes was quantified by Kruskal-Wallis analysis. By Mantel testing, the hierarchical clustering of the Fragaria genotypes based on associated EST or AFLP markers was compared to the observed eco-physiological relevant grouping. A better discriminating capacity for associated markers was noted, enabling a functional marker selection approach to screen the strawberry gene pool for drought tolerance. Correlation of EST markers to leaf RWC and WLR enforces them as potential candidate genes in control of plant responses to drought stress in Fragaria sp.},
  author       = {Razavi Hosseinabadi, Farzaneh and De Keyser, Ellen and De Riek, Jan and Van Labeke, Marie-Christine},
  issn         = {0014-2336},
  journal      = {EUPHYTICA},
  keyword      = {Genetic relationship,Clustering analysis,AFLP markers,EST marker,Water deficit,Drought stress,Strawberry cultivar,Fragaria vesca,Fragaria chiloensis,Fragaria x ananassa},
  language     = {eng},
  number       = {3},
  pages        = {385--409},
  title        = {A method for testing drought tolerance in Fragaria based on fast screening for water deficit response and use of associated AFLP and EST candidate gene markers},
  url          = {http://dx.doi.org/10.1007/s10681-011-0398-x},
  volume       = {180},
  year         = {2011},
}

Chicago
Razavi Hosseinabadi, Farzaneh, Ellen De Keyser, Jan De Riek, and Marie-Christine Van Labeke. 2011. “A Method for Testing Drought Tolerance in Fragaria Based on Fast Screening for Water Deficit Response and Use of Associated AFLP and EST Candidate Gene Markers.” Euphytica 180 (3): 385–409.
APA
Razavi Hosseinabadi, F., De Keyser, E., De Riek, J., & Van Labeke, M.-C. (2011). A method for testing drought tolerance in Fragaria based on fast screening for water deficit response and use of associated AFLP and EST candidate gene markers. EUPHYTICA, 180(3), 385–409.
Vancouver
1.
Razavi Hosseinabadi F, De Keyser E, De Riek J, Van Labeke M-C. A method for testing drought tolerance in Fragaria based on fast screening for water deficit response and use of associated AFLP and EST candidate gene markers. EUPHYTICA. 2011;180(3):385–409.
MLA
Razavi Hosseinabadi, Farzaneh, Ellen De Keyser, Jan De Riek, et al. “A Method for Testing Drought Tolerance in Fragaria Based on Fast Screening for Water Deficit Response and Use of Associated AFLP and EST Candidate Gene Markers.” EUPHYTICA 180.3 (2011): 385–409. Print.