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Chronic smoke exposure is associated with autophagy in murine Peyer's patches

STEPHANIE VERSCHUERE, Ken Bracke UGent, Rebecca De Smet, Liesbeth Allais, Guy Brusselle UGent and Claude Cuvelier (2011) United European Gastroenterology Week, Posters.
abstract
INTRODUCTION: Cigarette smoke causes oxidative stress, leading to smoke-induced autophagy in several organs. Autophagy is a homeostatic process regulating the turnover of proteins and cytoplasmatic organelles. However, recently it has also been associated with many autoimmune and inflammatory disorders, among which Crohn’s disease. The purpose of the present study was to investigate whether cigarette smoke exposure is associated with increased autophagy in Peyer’s patches and its epithelium. AIMS & METHODS: C57BL/6 mice were exposed to cigarette smoke or air. After 24 weeks, the animals were sacrificied and Peyer’s patches were collected. m RNA expression of autophagy-related genes was determined by RT-PCR. Transmission electron microscopy (TEM) was used to evaluate the presence of autophagic vesicles in the follicleassociated epithelium of Peyer’s patches. RESULTS: Expression of Beclin-1, a protein involved in the nucleation of autophagosomes, and of Atg5 and Atg7, which both play a role in the autophagosome vesicle elongation and completion, increased after chronic smoke exposure. Furthermore, electron microscopy of the follicle-associated epithelium demonstrated that the mean area of autophagic vesicles per epithelial cell increased considerably from 1.1 μm2 ± 0.4 μm2 in the air group to 2.4 μm2 ± 0.4 μm2 in the smoke group (p < 0.05). Epithelial cells had a significantly higher number of autophagic vesicles after smoke exposure (1.1 ± 0.1 after smoke exposure versus 0.5 ± 0.1 vesicles per cell after air exposure, p < 0.05), but the size of the vesicles did not differ between both groups. CONCLUSION: Here we provide the first evidence that chronic exposure to cigarette smoke is associated with autophagy in murine Peyer’s patches, and more in particular in the follicle-associated epithelium covering Peyer’s patches. Our findings can help to understand the role of smoking in the pathogenesis of inflammatory bowel disease, such as Crohn’s disease.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
autophagy, Cigarette smoke, Peyer's patches
in
United European Gastroenterology Week, Posters
conference name
United European Gastroenterology Week 2011 (UEGW 2011)
conference location
Stockholm, Sweden
conference start
2011-10-24
conference end
2011-10-26
language
English
UGent publication?
yes
classification
C3
copyright statement
I have retained and own the full copyright for this publication
id
1944614
handle
http://hdl.handle.net/1854/LU-1944614
date created
2011-11-20 11:16:44
date last changed
2016-12-19 15:35:22
@inproceedings{1944614,
  abstract     = {INTRODUCTION: Cigarette smoke causes oxidative stress, leading to smoke-induced autophagy in several organs. Autophagy is a homeostatic process regulating the turnover of proteins and cytoplasmatic organelles. However, recently it has also been associated with many autoimmune and inflammatory disorders, among which Crohn{\textquoteright}s disease. The purpose of the present study was to investigate whether cigarette smoke exposure is associated with increased autophagy in Peyer{\textquoteright}s patches and its epithelium.
AIMS \& METHODS: C57BL/6 mice were exposed to cigarette smoke or air. After 24 weeks, the animals were sacrificied and Peyer{\textquoteright}s patches were collected. m RNA expression of autophagy-related genes was determined by RT-PCR. Transmission electron microscopy (TEM) was used to evaluate the presence of autophagic vesicles in the follicleassociated epithelium of Peyer{\textquoteright}s patches.
RESULTS: Expression of Beclin-1, a protein involved in the nucleation of autophagosomes, and of Atg5 and Atg7, which both play a role in the autophagosome vesicle elongation and completion, increased after chronic smoke exposure. Furthermore, electron microscopy of the follicle-associated epithelium demonstrated that the mean area of autophagic vesicles per epithelial cell increased considerably from 1.1 \ensuremath{\mu}m2 {\textpm} 0.4 \ensuremath{\mu}m2 in the air group to 2.4 \ensuremath{\mu}m2 {\textpm} 0.4 \ensuremath{\mu}m2 in the smoke group (p {\textlangle} 0.05). Epithelial cells had a significantly higher number of autophagic vesicles after smoke exposure (1.1 {\textpm} 0.1 after smoke exposure versus 0.5 {\textpm} 0.1 vesicles per cell after air exposure, p {\textlangle} 0.05), but the size of the vesicles did not differ between both groups.
CONCLUSION: Here we provide the first evidence that chronic exposure to cigarette smoke is associated with autophagy in murine Peyer{\textquoteright}s patches, and more in particular in the follicle-associated epithelium covering Peyer{\textquoteright}s patches. Our findings can help to understand the role of smoking in the pathogenesis of inflammatory bowel disease, such as Crohn{\textquoteright}s disease.},
  author       = {VERSCHUERE, STEPHANIE and Bracke, Ken and De Smet, Rebecca and Allais, Liesbeth and Brusselle, Guy and Cuvelier, Claude},
  booktitle    = {United European Gastroenterology Week, Posters},
  keyword      = {autophagy,Cigarette smoke,Peyer's patches},
  language     = {eng},
  location     = {Stockholm, Sweden},
  title        = {Chronic smoke exposure is associated with autophagy in murine Peyer's patches},
  year         = {2011},
}

Chicago
VERSCHUERE, STEPHANIE, Ken Bracke, Rebecca De Smet, Liesbeth Allais, Guy Brusselle, and Claude Cuvelier. 2011. “Chronic Smoke Exposure Is Associated with Autophagy in Murine Peyer’s Patches.” In United European Gastroenterology Week, Posters.
APA
VERSCHUERE, S., Bracke, K., De Smet, R., Allais, L., Brusselle, G., & Cuvelier, C. (2011). Chronic smoke exposure is associated with autophagy in murine Peyer’s patches. United European Gastroenterology Week, Posters. Presented at the United European Gastroenterology Week 2011 (UEGW 2011).
Vancouver
1.
VERSCHUERE S, Bracke K, De Smet R, Allais L, Brusselle G, Cuvelier C. Chronic smoke exposure is associated with autophagy in murine Peyer’s patches. United European Gastroenterology Week, Posters. 2011.
MLA
VERSCHUERE, STEPHANIE, Ken Bracke, Rebecca De Smet, et al. “Chronic Smoke Exposure Is Associated with Autophagy in Murine Peyer’s Patches.” United European Gastroenterology Week, Posters. 2011. Print.