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The jasmonate pathway is a key player in systemically induced defense against root knot nematodes in rice

Kamrun Nahar UGent, Tina Kyndt UGent, David De Vleesschauwer UGent, Monica Höfte UGent and Godelieve Gheysen UGent (2011) PLANT PHYSIOLOGY. 157(1). p.305-316
abstract
Complex defense signaling pathways, controlled by different hormones, are involved in the reaction of plants to a wide range of biotic and abiotic stress factors. We studied the ability of salicylic acid (SA), jasmonate (JA) and ethylene (ET) to induce systemic defense in rice (Oryza sativa) against the root knot nematode (RKN) Meloidogyne graminicola. Exogenous ET (ethephon) and JA (methyl jasmonate) supply on the shoots induced a strong systemic defense response in the roots, exemplified by a major upregulation of OsPR1a and OsPR1b, while the SA-analogue BTH was a less potent systemic defense inducer from shoot to root. Experiments with JA-biosynthesis mutants and ET-insensitive transgenics showed that ET induced defense requires an intact JA-pathway while JA-induced defense was still functional when ET-signaling was impaired. Pharmacological inhibition of JA and ET-biosynthesis confirmed that JA-biosynthesis is needed for ET-induced systemic defense and qRT-PCR data revealed that ET application onto the shoots strongly activates jasmonate biosynthesis and signaling genes in the roots. All data provided in this study point to the JA-pathway to play a pivotal role in rice defense against RKN. The expression of defense-related genes was monitored in root galls caused by M. graminicola. Different analyzed defense genes were attenuated in root galls caused by the nematode at early timepoints after infection. However, when exogenous defense inducers ethephon and MeJA were supplied to the plant, the nematode was less effective in counteracting root defense pathways, hence making the plant more resistant to nematode infection.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ACID SIGNALING PATHWAY, PLANT-PARASITIC NEMATODES, SALICYLIC-ACID, CYST-NEMATODE, ARABIDOPSIS-THALIANA, DISEASE RESISTANCE, GENE-EXPRESSION, TRANSCRIPTION FACTOR, FUNGAL-INFECTION, FEEDING CELLS
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
157
issue
1
pages
305 - 316
Web of Science type
Article
Web of Science id
000294491800024
JCR category
PLANT SCIENCES
JCR impact factor
6.535 (2011)
JCR rank
7/189 (2011)
JCR quartile
1 (2011)
ISSN
0032-0889
DOI
10.1104/pp.111.177576
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1942154
handle
http://hdl.handle.net/1854/LU-1942154
date created
2011-11-10 16:04:49
date last changed
2011-11-16 14:18:21
@article{1942154,
  abstract     = {Complex defense signaling pathways, controlled by different hormones, are involved in the reaction of plants to a wide range of biotic and abiotic stress factors. We studied the ability of salicylic acid (SA), jasmonate (JA) and ethylene (ET) to induce systemic defense in rice (Oryza sativa) against the root knot nematode (RKN) Meloidogyne graminicola. Exogenous ET (ethephon) and JA (methyl jasmonate) supply on the shoots induced a strong systemic defense response in the roots, exemplified by a major upregulation of OsPR1a and OsPR1b, while the SA-analogue BTH was a less potent systemic defense inducer from shoot to root. Experiments with JA-biosynthesis mutants and ET-insensitive transgenics showed that ET induced defense requires an intact JA-pathway while JA-induced defense was still functional when ET-signaling was impaired. Pharmacological inhibition of JA and ET-biosynthesis confirmed that JA-biosynthesis is needed for ET-induced systemic defense and qRT-PCR data revealed that ET application onto the shoots strongly activates jasmonate biosynthesis and signaling genes in the roots. All data provided in this study point to the JA-pathway to play a pivotal role in rice defense against RKN. The expression of defense-related genes was monitored in root galls caused by M. graminicola. Different analyzed defense genes were attenuated in root galls caused by the nematode at early timepoints after infection. However, when exogenous defense inducers ethephon and MeJA were supplied to the plant, the nematode was less effective in counteracting root defense pathways, hence making the plant more resistant to nematode infection.},
  author       = {Nahar, Kamrun and Kyndt, Tina and De Vleesschauwer, David and H{\"o}fte, Monica and Gheysen, Godelieve},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {ACID SIGNALING PATHWAY,PLANT-PARASITIC NEMATODES,SALICYLIC-ACID,CYST-NEMATODE,ARABIDOPSIS-THALIANA,DISEASE RESISTANCE,GENE-EXPRESSION,TRANSCRIPTION FACTOR,FUNGAL-INFECTION,FEEDING CELLS},
  language     = {eng},
  number       = {1},
  pages        = {305--316},
  title        = {The jasmonate pathway is a key player in systemically induced defense against root knot nematodes in rice},
  url          = {http://dx.doi.org/10.1104/pp.111.177576},
  volume       = {157},
  year         = {2011},
}

Chicago
Nahar, Kamrun, Tina Kyndt, David De Vleesschauwer, Monica Höfte, and Godelieve Gheysen. 2011. “The Jasmonate Pathway Is a Key Player in Systemically Induced Defense Against Root Knot Nematodes in Rice.” Plant Physiology 157 (1): 305–316.
APA
Nahar, K., Kyndt, T., De Vleesschauwer, D., Höfte, M., & Gheysen, G. (2011). The jasmonate pathway is a key player in systemically induced defense against root knot nematodes in rice. PLANT PHYSIOLOGY, 157(1), 305–316.
Vancouver
1.
Nahar K, Kyndt T, De Vleesschauwer D, Höfte M, Gheysen G. The jasmonate pathway is a key player in systemically induced defense against root knot nematodes in rice. PLANT PHYSIOLOGY. 2011;157(1):305–16.
MLA
Nahar, Kamrun, Tina Kyndt, David De Vleesschauwer, et al. “The Jasmonate Pathway Is a Key Player in Systemically Induced Defense Against Root Knot Nematodes in Rice.” PLANT PHYSIOLOGY 157.1 (2011): 305–316. Print.