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Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin

Katrijn Vannerum (UGent) , Marie Huysman (UGent) , Riet De Rycke (UGent) , Marnik Vuylsteke (UGent) , Frédérik Leliaert (UGent) , Jacob Pollier (UGent) , Ursula Lutz-Meindl, Jeroen Gillard (UGent) , Lieven De Veylder (UGent) , Alain Goossens (UGent) , et al.
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Background: Streptophyte green algae share several characteristics of cell growth and cell wall formation with their relatives, the embryophytic land plants. The multilobed cell wall of Micrasterias denticulata that rebuilds symmetrically after cell division and consists of pectin and cellulose, makes this unicellular streptophyte alga an interesting model system to study the molecular controls on cell shape and cell wall formation in green plants. Results: Genome-wide transcript expression profiling of synchronously growing cells identified 107 genes of which the expression correlated with the growth phase. Four transcripts showed high similarity to expansins that had not been examined previously in green algae. Phylogenetic analysis suggests that these genes are most closely related to the plant EXPANSIN A family, although their domain organization is very divergent. A GFP-tagged version of the expansin-resembling protein MdEXP2 localized to the cell wall and in Golgi-derived vesicles. Overexpression phenotypes ranged from lobe elongation to loss of growth polarity and planarity. These results indicate that MdEXP2 can alter the cell wall structure and, thus, might have a function related to that of land plant expansins during cell morphogenesis. Conclusions: Our study demonstrates the potential of M. denticulata as a unicellular model system, in which cell growth mechanisms have been discovered similar to those in land plants. Additionally, evidence is provided that the evolutionary origins of many cell wall components and regulatory genes in embryophytes precede the colonization of land.
Keywords
SIGNAL PEPTIDES, CHLOROPLAST GENOME, CELLULOSE SYNTHASE, EVOLUTIONARY ORIGIN, ARABIDOPSIS-THALIANA, GENOME-WIDE EXPRESSION, LAND PLANTS, GENE-EXPRESSION, CDNA-AFLP, WALLS

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Chicago
Vannerum, Katrijn, Marie Huysman, Riet De Rycke, Marnik Vuylsteke, Frédérik Leliaert, Jacob Pollier, Ursula Lutz-Meindl, et al. 2011. “Transcriptional Analysis of Cell Growth and Morphogenesis in the Unicellular Green Alga Micrasterias (Streptophyta), with Emphasis on the Role of Expansin.” Bmc Plant Biology 11.
APA
Vannerum, K., Huysman, M., De Rycke, R., Vuylsteke, M., Leliaert, F., Pollier, J., Lutz-Meindl, U., et al. (2011). Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin. BMC PLANT BIOLOGY, 11.
Vancouver
1.
Vannerum K, Huysman M, De Rycke R, Vuylsteke M, Leliaert F, Pollier J, et al. Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin. BMC PLANT BIOLOGY. 2011;11.
MLA
Vannerum, Katrijn, Marie Huysman, Riet De Rycke, et al. “Transcriptional Analysis of Cell Growth and Morphogenesis in the Unicellular Green Alga Micrasterias (Streptophyta), with Emphasis on the Role of Expansin.” BMC PLANT BIOLOGY 11 (2011): n. pag. Print.
@article{1939620,
  abstract     = {Background: Streptophyte green algae share several characteristics of cell growth and cell wall formation with their relatives, the embryophytic land plants. The multilobed cell wall of Micrasterias denticulata that rebuilds symmetrically after cell division and consists of pectin and cellulose, makes this unicellular streptophyte alga an interesting model system to study the molecular controls on cell shape and cell wall formation in green plants.
Results: Genome-wide transcript expression profiling of synchronously growing cells identified 107 genes of which the expression correlated with the growth phase. Four transcripts showed high similarity to expansins that had not been examined previously in green algae. Phylogenetic analysis suggests that these genes are most closely related to the plant EXPANSIN A family, although their domain organization is very divergent. A GFP-tagged version of the expansin-resembling protein MdEXP2 localized to the cell wall and in Golgi-derived vesicles. Overexpression phenotypes ranged from lobe elongation to loss of growth polarity and planarity. These results indicate that MdEXP2 can alter the cell wall structure and, thus, might have a function related to that of land plant expansins during cell morphogenesis.
Conclusions: Our study demonstrates the potential of M. denticulata as a unicellular model system, in which cell growth mechanisms have been discovered similar to those in land plants. Additionally, evidence is provided that the evolutionary origins of many cell wall components and regulatory genes in embryophytes precede the colonization of land.},
  articleno    = {128},
  author       = {Vannerum, Katrijn and Huysman, Marie and De Rycke, Riet and Vuylsteke, Marnik and Leliaert, Fr{\'e}d{\'e}rik and Pollier, Jacob and Lutz-Meindl, Ursula and Gillard, Jeroen and De Veylder, Lieven and Goossens, Alain and Inz{\'e}, Dirk and Vyverman, Wim},
  issn         = {1471-2229},
  journal      = {BMC PLANT BIOLOGY},
  keyword      = {SIGNAL PEPTIDES,CHLOROPLAST GENOME,CELLULOSE SYNTHASE,EVOLUTIONARY ORIGIN,ARABIDOPSIS-THALIANA,GENOME-WIDE EXPRESSION,LAND PLANTS,GENE-EXPRESSION,CDNA-AFLP,WALLS},
  language     = {eng},
  pages        = {17},
  title        = {Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin},
  url          = {http://dx.doi.org/10.1186/1471-2229-11-128},
  volume       = {11},
  year         = {2011},
}

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