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Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin

Katrijn Vannerum UGent, Marie Huysman, Riet De Rycke UGent, Marnik Vuylsteke UGent, Frédérik Leliaert UGent, Jacob Pollier UGent, Ursula Lutz-Meindl, Jeroen Gillard UGent, Lieven De Veylder UGent, Alain Goossens UGent, et al. (2011) BMC PLANT BIOLOGY. 11.
abstract
Background: Streptophyte green algae share several characteristics of cell growth and cell wall formation with their relatives, the embryophytic land plants. The multilobed cell wall of Micrasterias denticulata that rebuilds symmetrically after cell division and consists of pectin and cellulose, makes this unicellular streptophyte alga an interesting model system to study the molecular controls on cell shape and cell wall formation in green plants. Results: Genome-wide transcript expression profiling of synchronously growing cells identified 107 genes of which the expression correlated with the growth phase. Four transcripts showed high similarity to expansins that had not been examined previously in green algae. Phylogenetic analysis suggests that these genes are most closely related to the plant EXPANSIN A family, although their domain organization is very divergent. A GFP-tagged version of the expansin-resembling protein MdEXP2 localized to the cell wall and in Golgi-derived vesicles. Overexpression phenotypes ranged from lobe elongation to loss of growth polarity and planarity. These results indicate that MdEXP2 can alter the cell wall structure and, thus, might have a function related to that of land plant expansins during cell morphogenesis. Conclusions: Our study demonstrates the potential of M. denticulata as a unicellular model system, in which cell growth mechanisms have been discovered similar to those in land plants. Additionally, evidence is provided that the evolutionary origins of many cell wall components and regulatory genes in embryophytes precede the colonization of land.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SIGNAL PEPTIDES, CHLOROPLAST GENOME, CELLULOSE SYNTHASE, EVOLUTIONARY ORIGIN, ARABIDOPSIS-THALIANA, GENOME-WIDE EXPRESSION, LAND PLANTS, GENE-EXPRESSION, CDNA-AFLP, WALLS
journal title
BMC PLANT BIOLOGY
BMC Plant Biol.
volume
11
article number
128
pages
17 pages
Web of Science type
Article
Web of Science id
000295789100001
JCR category
PLANT SCIENCES
JCR impact factor
3.447 (2011)
JCR rank
21/189 (2011)
JCR quartile
1 (2011)
ISSN
1471-2229
DOI
10.1186/1471-2229-11-128
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1939620
handle
http://hdl.handle.net/1854/LU-1939620
date created
2011-11-07 17:36:05
date last changed
2016-12-21 15:41:50
@article{1939620,
  abstract     = {Background: Streptophyte green algae share several characteristics of cell growth and cell wall formation with their relatives, the embryophytic land plants. The multilobed cell wall of Micrasterias denticulata that rebuilds symmetrically after cell division and consists of pectin and cellulose, makes this unicellular streptophyte alga an interesting model system to study the molecular controls on cell shape and cell wall formation in green plants.
Results: Genome-wide transcript expression profiling of synchronously growing cells identified 107 genes of which the expression correlated with the growth phase. Four transcripts showed high similarity to expansins that had not been examined previously in green algae. Phylogenetic analysis suggests that these genes are most closely related to the plant EXPANSIN A family, although their domain organization is very divergent. A GFP-tagged version of the expansin-resembling protein MdEXP2 localized to the cell wall and in Golgi-derived vesicles. Overexpression phenotypes ranged from lobe elongation to loss of growth polarity and planarity. These results indicate that MdEXP2 can alter the cell wall structure and, thus, might have a function related to that of land plant expansins during cell morphogenesis.
Conclusions: Our study demonstrates the potential of M. denticulata as a unicellular model system, in which cell growth mechanisms have been discovered similar to those in land plants. Additionally, evidence is provided that the evolutionary origins of many cell wall components and regulatory genes in embryophytes precede the colonization of land.},
  articleno    = {128},
  author       = {Vannerum, Katrijn and Huysman, Marie and De Rycke, Riet and Vuylsteke, Marnik and Leliaert, Fr{\'e}d{\'e}rik and Pollier, Jacob and Lutz-Meindl, Ursula and Gillard, Jeroen and De Veylder, Lieven and Goossens, Alain and Inz{\'e}, Dirk and Vyverman, Wim},
  issn         = {1471-2229},
  journal      = {BMC PLANT BIOLOGY},
  keyword      = {SIGNAL PEPTIDES,CHLOROPLAST GENOME,CELLULOSE SYNTHASE,EVOLUTIONARY ORIGIN,ARABIDOPSIS-THALIANA,GENOME-WIDE EXPRESSION,LAND PLANTS,GENE-EXPRESSION,CDNA-AFLP,WALLS},
  language     = {eng},
  pages        = {17},
  title        = {Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin},
  url          = {http://dx.doi.org/10.1186/1471-2229-11-128},
  volume       = {11},
  year         = {2011},
}

Chicago
Vannerum, Katrijn, Marie Huysman, Riet De Rycke, Marnik Vuylsteke, Frédérik Leliaert, Jacob Pollier, Ursula Lutz-Meindl, et al. 2011. “Transcriptional Analysis of Cell Growth and Morphogenesis in the Unicellular Green Alga Micrasterias (Streptophyta), with Emphasis on the Role of Expansin.” Bmc Plant Biology 11.
APA
Vannerum, K., Huysman, M., De Rycke, R., Vuylsteke, M., Leliaert, F., Pollier, J., Lutz-Meindl, U., et al. (2011). Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin. BMC PLANT BIOLOGY, 11.
Vancouver
1.
Vannerum K, Huysman M, De Rycke R, Vuylsteke M, Leliaert F, Pollier J, et al. Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin. BMC PLANT BIOLOGY. 2011;11.
MLA
Vannerum, Katrijn, Marie Huysman, Riet De Rycke, et al. “Transcriptional Analysis of Cell Growth and Morphogenesis in the Unicellular Green Alga Micrasterias (Streptophyta), with Emphasis on the Role of Expansin.” BMC PLANT BIOLOGY 11 (2011): n. pag. Print.