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Antimicrobial resistance in Swiss laying hens, prevalence and risk factors

M Harisberger, S Gobeli, R Hoop, Jeroen Dewulf UGent, V Perreten and G Regula (2011) ZOONOSES AND PUBLIC HEALTH. 58(6). p.377-387
abstract
Antimicrobial resistance is an emerging concern to public health, and food-producing animals are known to be a potential source for transmission of resistant bacteria to humans. As legislation of the European Union requires to ban conventional cages for the housing of laying hens on the one hand, and a high food safety standard for eggs on the other hand, further investigations about the occurrence of antimicrobial resistance in alternative housing types are required. In this study, we determined antimicrobial resistance in indicator bacteria from 396 cloacal swabs from 99 Swiss laying hen farms among four alternative housing types during a cross-sectional study. On each farm, four hens were sampled and exposure to potential risk factors was identified with a questionnaire. The minimal inhibitory concentration was determined using broth microdilution in Escherichia coli (n = 371) for 18 antimicrobials and in Enterococcus faecalis (n = 138) and Enterococcus faecium (n = 153) for 16 antimicrobials. All antimicrobial classes recommended by the European Food Safety Authority for E. coli and enterococci were included in the resistance profile. Sixty per cent of the E. coli isolates were susceptible to all of the considered antimicrobials and 30% were resistant to at least two antimicrobials. In E. faecalis, 33% of the strains were susceptible to all tested antimicrobials and 40% were resistant to two or more antimicrobials, whereas in E. faecium these figures were 14% and 39% respectively. Risk factor analyses were carried out for bacteria species and antimicrobials with a prevalence of resistance between 15% and 85%. In these analyses, none of the considered housing and management factors showed a consistent association with the prevalence of resistance for more than two combinations of bacteria and antimicrobial. Therefore we conclude that the impact of the considered housing and management practices on the egg producing farms on resistance in laying hens is low.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Escherichia coli, Enterococcus faecalis, Enterococcus faecium, Switzerland, housing type, Safehouse project, ESCHERICHIA-COLI, SALMONELLA-ENTERITIDIS, ANTIBIOTIC-RESISTANCE, POULTRY, EGGS, SWITZERLAND
journal title
ZOONOSES AND PUBLIC HEALTH
Zoonoses Public Health
volume
58
issue
6
pages
377 - 387
Web of Science type
Article
Web of Science id
000293711300001
JCR category
VETERINARY SCIENCES
JCR impact factor
1.895 (2011)
JCR rank
21/141 (2011)
JCR quartile
1 (2011)
ISSN
1863-1959
DOI
10.1111/j.1863-2378.2010.01376.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1934030
handle
http://hdl.handle.net/1854/LU-1934030
date created
2011-10-21 10:18:12
date last changed
2016-12-19 15:39:52
@article{1934030,
  abstract     = {Antimicrobial resistance is an emerging concern to public health, and food-producing animals are known to be a potential source for transmission of resistant bacteria to humans. As legislation of the European Union requires to ban conventional cages for the housing of laying hens on the one hand, and a high food safety standard for eggs on the other hand, further investigations about the occurrence of antimicrobial resistance in alternative housing types are required. In this study, we determined antimicrobial resistance in indicator bacteria from 396 cloacal swabs from 99 Swiss laying hen farms among four alternative housing types during a cross-sectional study. On each farm, four hens were sampled and exposure to potential risk factors was identified with a questionnaire. The minimal inhibitory concentration was determined using broth microdilution in Escherichia coli (n = 371) for 18 antimicrobials and in Enterococcus faecalis (n = 138) and Enterococcus faecium (n = 153) for 16 antimicrobials. All antimicrobial classes recommended by the European Food Safety Authority for E. coli and enterococci were included in the resistance profile. Sixty per cent of the E. coli isolates were susceptible to all of the considered antimicrobials and 30\% were resistant to at least two antimicrobials. In E. faecalis, 33\% of the strains were susceptible to all tested antimicrobials and 40\% were resistant to two or more antimicrobials, whereas in E. faecium these figures were 14\% and 39\% respectively. Risk factor analyses were carried out for bacteria species and antimicrobials with a prevalence of resistance between 15\% and 85\%. In these analyses, none of the considered housing and management factors showed a consistent association with the prevalence of resistance for more than two combinations of bacteria and antimicrobial. Therefore we conclude that the impact of the considered housing and management practices on the egg producing farms on resistance in laying hens is low.},
  author       = {Harisberger, M and Gobeli, S and Hoop, R and Dewulf, Jeroen and Perreten, V and Regula, G},
  issn         = {1863-1959},
  journal      = {ZOONOSES AND PUBLIC HEALTH},
  keyword      = {Escherichia coli,Enterococcus faecalis,Enterococcus faecium,Switzerland,housing type,Safehouse project,ESCHERICHIA-COLI,SALMONELLA-ENTERITIDIS,ANTIBIOTIC-RESISTANCE,POULTRY,EGGS,SWITZERLAND},
  language     = {eng},
  number       = {6},
  pages        = {377--387},
  title        = {Antimicrobial resistance in Swiss laying hens, prevalence and risk factors},
  url          = {http://dx.doi.org/10.1111/j.1863-2378.2010.01376.x},
  volume       = {58},
  year         = {2011},
}

Chicago
Harisberger, M, S Gobeli, R Hoop, Jeroen Dewulf, V Perreten, and G Regula. 2011. “Antimicrobial Resistance in Swiss Laying Hens, Prevalence and Risk Factors.” Zoonoses and Public Health 58 (6): 377–387.
APA
Harisberger, M., Gobeli, S., Hoop, R., Dewulf, J., Perreten, V., & Regula, G. (2011). Antimicrobial resistance in Swiss laying hens, prevalence and risk factors. ZOONOSES AND PUBLIC HEALTH, 58(6), 377–387.
Vancouver
1.
Harisberger M, Gobeli S, Hoop R, Dewulf J, Perreten V, Regula G. Antimicrobial resistance in Swiss laying hens, prevalence and risk factors. ZOONOSES AND PUBLIC HEALTH. 2011;58(6):377–87.
MLA
Harisberger, M, S Gobeli, R Hoop, et al. “Antimicrobial Resistance in Swiss Laying Hens, Prevalence and Risk Factors.” ZOONOSES AND PUBLIC HEALTH 58.6 (2011): 377–387. Print.