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Sources other than unused sawdust can introduce Klebsiella pneumoniae into dairy herds

Bert Verbist UGent, Veerle Piessens UGent, A Van Nuffel, L De Vuyst, Marc Heyndrickx UGent, Lieve Herman, E Van Coillie and Sarne De Vliegher UGent (2011) JOURNAL OF DAIRY SCIENCE. 94(6). p.2832-2839
abstract
A longitudinal study was carried out to detect intramammary infections caused by Klebsiella pneumoniae and to identify potential sources of this bacterial species in the environment of the cows. The study was performed in 6 well-managed Belgian dairy herds from May 2008 to May 2009. Monthly (n = 13), unused and used sawdust bedding samples as well as individual quarter milk and feces samples were collected from 10 randomly selected cohort cows in each herd. Cases of clinical mastitis of all lactating cows in the 6 herds were also sampled (n = 64). From the 3,518 collected samples, 153 K. pneumoniae isolates were obtained, of which 2 originated from milk (clinical mastitis cases). In feces (n = 728), used bedding (n = 73), and unused bedding (n = 73), respectively, 125 (17.2%), 20 (27.4%), and 6 (8.2%) isolates were found. The isolates were fingerprinted by means of pulsed field gel electrophoresis. In total, 109 different pulsotypes were differentiated, indicating a high degree of genetic diversity within the isolates. All isolates from unused bedding belonged to pulsotypes other than those from the other sources, suggesting that sources other than unused sawdust may introduce K. pneumoniae into the herd. Only 2 pulsotypes contained isolates originating from different sources. Pulsotype 10 was found in milk and used bedding and pulsotype 21 was found in feces and used bedding. The 2 milk isolates originated from 2 cows in the same herd but they belonged to a different pulsotype. The results indicate that K. pneumoniae can be prevalent in the environment without causing significant mastitis problems. Most cows were shedding K. pneumoniae in feces, substantiating findings under very different conditions (i.e., American dairy herds). Contamination of used bedding in the cubicles with K. pneumoniae from feces was confirmed, whereas unused bedding was not an important source of K. pneumoniae for the environment of the cows.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
intramammary infection, environment, Klebsiella pneumoniae, pulsed field gel electrophoresis, CLINICAL MASTITIS, BOVINE MASTITIS, COWS, EFFICACY, DIVERSITY, PATHOGENS, COUNTS
journal title
JOURNAL OF DAIRY SCIENCE
J. Dairy Sci.
volume
94
issue
6
pages
2832 - 2839
Web of Science type
Article
Web of Science id
000290777800018
JCR category
AGRICULTURE, DAIRY & ANIMAL SCIENCE
JCR impact factor
2.564 (2011)
JCR rank
2/54 (2011)
JCR quartile
1 (2011)
ISSN
0022-0302
DOI
10.3168/jds.2010-3700
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1930620
handle
http://hdl.handle.net/1854/LU-1930620
date created
2011-10-17 14:18:49
date last changed
2011-10-18 10:59:24
@article{1930620,
  abstract     = {A longitudinal study was carried out to detect intramammary infections caused by Klebsiella pneumoniae and to identify potential sources of this bacterial species in the environment of the cows. The study was performed in 6 well-managed Belgian dairy herds from May 2008 to May 2009. Monthly (n = 13), unused and used sawdust bedding samples as well as individual quarter milk and feces samples were collected from 10 randomly selected cohort cows in each herd. Cases of clinical mastitis of all lactating cows in the 6 herds were also sampled (n = 64). From the 3,518 collected samples, 153 K. pneumoniae isolates were obtained, of which 2 originated from milk (clinical mastitis cases). In feces (n = 728), used bedding (n = 73), and unused bedding (n = 73), respectively, 125 (17.2\%), 20 (27.4\%), and 6 (8.2\%) isolates were found. The isolates were fingerprinted by means of pulsed field gel electrophoresis. In total, 109 different pulsotypes were differentiated, indicating a high degree of genetic diversity within the isolates. All isolates from unused bedding belonged to pulsotypes other than those from the other sources, suggesting that sources other than unused sawdust may introduce K. pneumoniae into the herd. Only 2 pulsotypes contained isolates originating from different sources. Pulsotype 10 was found in milk and used bedding and pulsotype 21 was found in feces and used bedding. The 2 milk isolates originated from 2 cows in the same herd but they belonged to a different pulsotype. The results indicate that K. pneumoniae can be prevalent in the environment without causing significant mastitis problems. Most cows were shedding K. pneumoniae in feces, substantiating findings under very different conditions (i.e., American dairy herds). Contamination of used bedding in the cubicles with K. pneumoniae from feces was confirmed, whereas unused bedding was not an important source of K. pneumoniae for the environment of the cows.},
  author       = {Verbist, Bert and Piessens, Veerle and Van Nuffel, A and De Vuyst, L and Heyndrickx, Marc and Herman, Lieve and Van Coillie, E and De Vliegher, Sarne},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keyword      = {intramammary infection,environment,Klebsiella pneumoniae,pulsed field gel electrophoresis,CLINICAL MASTITIS,BOVINE MASTITIS,COWS,EFFICACY,DIVERSITY,PATHOGENS,COUNTS},
  language     = {eng},
  number       = {6},
  pages        = {2832--2839},
  title        = {Sources other than unused sawdust can introduce Klebsiella pneumoniae into dairy herds},
  url          = {http://dx.doi.org/10.3168/jds.2010-3700},
  volume       = {94},
  year         = {2011},
}

Chicago
Verbist, Bert, Veerle Piessens, A Van Nuffel, L De Vuyst, Marc Heyndrickx, Lieve Herman, E Van Coillie, and Sarne De Vliegher. 2011. “Sources Other Than Unused Sawdust Can Introduce Klebsiella Pneumoniae into Dairy Herds.” Journal of Dairy Science 94 (6): 2832–2839.
APA
Verbist, B., Piessens, V., Van Nuffel, A., De Vuyst, L., Heyndrickx, M., Herman, L., Van Coillie, E., et al. (2011). Sources other than unused sawdust can introduce Klebsiella pneumoniae into dairy herds. JOURNAL OF DAIRY SCIENCE, 94(6), 2832–2839.
Vancouver
1.
Verbist B, Piessens V, Van Nuffel A, De Vuyst L, Heyndrickx M, Herman L, et al. Sources other than unused sawdust can introduce Klebsiella pneumoniae into dairy herds. JOURNAL OF DAIRY SCIENCE. 2011;94(6):2832–9.
MLA
Verbist, Bert, Veerle Piessens, A Van Nuffel, et al. “Sources Other Than Unused Sawdust Can Introduce Klebsiella Pneumoniae into Dairy Herds.” JOURNAL OF DAIRY SCIENCE 94.6 (2011): 2832–2839. Print.