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Transformation of chicory and expression of the bacterial uidA and nptll genes in the transgenic regenerants

(1995) JOURNAL OF EXPERIMENTAL BOTANY. 46(284). p.337-346
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Abstract
Transgenic chicory plants were obtained from different explants co-cultured with Agrobacterium tumefaciens. Among tap-root, leaf and cotyledonary tissues, etiolated cotyledons showed the greatest competence for transformation. The Agrobacterium strains used contained either pGSGLUC1 or pTDE4 as a vector which carry both the neomycin phosphotransferase ii gene (nptII) for kanamycin resistance and beta-glucuronidase gene (uidA) under the control of different promoters. Transformation was confirmed by NPTII enzymatic assay, histochemical analysis of GUS activity and DNA hybridization. Transgenic plants expressed both marker genes in root and shoot tissues. In leaves, GUS activity was expressed in all tissue types, whatever the nature of the promoter. Nevertheless, variable heterogeneous patterns of expression were observed in the different root tissues. Differential expression of the GUS fusions controlled by the dual Tn or the CaMV 35S promoters are discussed.
Keywords
SENSITIVE METHOD, BETA-GLUCURONIDASE, PLANTS, PROMOTER, EXPLANTS, FOREIGN GENES, AGROBACTERIUM-TUMEFACIENS, CICHORIUM-INTYBUS L, DIRECT SOMATIC EMBRYOGENESIS, TUMEFACIENS-MEDIATED TRANSFORMATION, GENETIC TRANSFORMATION, CHICORY, GUS ACTIVITY, KANAMYCIN RESISTANCE

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Citation

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Chicago
Abid, Malika, Benoît Palms, Riet De Rycke, Jean Pierre Tissier, and Serge Rambourg. 1995. “Transformation of Chicory and Expression of the Bacterial uidA and Nptll Genes in the Transgenic Regenerants.” Journal of Experimental Botany 46 (284): 337–346.
APA
Abid, Malika, Palms, B., De Rycke, R., Tissier, J. P., & Rambourg, S. (1995). Transformation of chicory and expression of the bacterial uidA and nptll genes in the transgenic regenerants. JOURNAL OF EXPERIMENTAL BOTANY, 46(284), 337–346.
Vancouver
1.
Abid M, Palms B, De Rycke R, Tissier JP, Rambourg S. Transformation of chicory and expression of the bacterial uidA and nptll genes in the transgenic regenerants. JOURNAL OF EXPERIMENTAL BOTANY. 1995;46(284):337–46.
MLA
Abid, Malika, Benoît Palms, Riet De Rycke, et al. “Transformation of Chicory and Expression of the Bacterial uidA and Nptll Genes in the Transgenic Regenerants.” JOURNAL OF EXPERIMENTAL BOTANY 46.284 (1995): 337–346. Print.
@article{192935,
  abstract     = {Transgenic chicory plants were obtained from different explants co-cultured with Agrobacterium tumefaciens. Among tap-root, leaf and cotyledonary tissues, etiolated cotyledons showed the greatest competence for transformation. The Agrobacterium strains used contained either pGSGLUC1 or pTDE4 as a vector which carry both the neomycin phosphotransferase ii gene (nptII) for kanamycin resistance and beta-glucuronidase gene (uidA) under the control of different promoters. Transformation was confirmed by NPTII enzymatic assay, histochemical analysis of GUS activity and DNA hybridization. Transgenic plants expressed both marker genes in root and shoot tissues. In leaves, GUS activity was expressed in all tissue types, whatever the nature of the promoter. Nevertheless, variable heterogeneous patterns of expression were observed in the different root tissues. 
Differential expression of the GUS fusions controlled by the dual Tn or the CaMV 35S promoters are discussed.},
  author       = {Abid, Malika and Palms, Beno{\^i}t and De Rycke, Riet and Tissier, Jean Pierre and Rambourg, Serge},
  issn         = {0022-0957},
  journal      = {JOURNAL OF EXPERIMENTAL BOTANY},
  keyword      = {SENSITIVE METHOD,BETA-GLUCURONIDASE,PLANTS,PROMOTER,EXPLANTS,FOREIGN GENES,AGROBACTERIUM-TUMEFACIENS,CICHORIUM-INTYBUS L,DIRECT SOMATIC EMBRYOGENESIS,TUMEFACIENS-MEDIATED TRANSFORMATION,GENETIC TRANSFORMATION,CHICORY,GUS ACTIVITY,KANAMYCIN RESISTANCE},
  language     = {eng},
  number       = {284},
  pages        = {337--346},
  title        = {Transformation of chicory and expression of the bacterial uidA and nptll genes in the transgenic regenerants},
  url          = {http://dx.doi.org/10.1093/jxb/46.3.337},
  volume       = {46},
  year         = {1995},
}

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