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Factors affecting the enhancement of oxidative stress tolerance in transgenic tobacco overexpressing manganese superoxide dismutase in the chloroplasts

(1995) PLANT PHYSIOLOGY. 107(3). p.737-750
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Abstract
Two varieties of tobacco (Nicotiana tabacum var PBD6 and var SR1) were used to generate transgenic lines overexpressing Mn-superoxide dismutase (MnSOD) in the chloroplasts. The overexpressed MnSOD suppresses the activity of those SODs (endogenous MnSOD and chloroplastic and cytosolic Cu/ZnSOD) that are prominent in young leaves but disappear largely or completely during aging of the leaves. The transgenic and control plants were grown at different light intensities and were then assayed for oxygen radical stress tolerance in leaf disc assays and for abundance of antioxidant enzymes and substrates in leaves. Transgenic plants had an enhanced resistance to methylviologen (MV), compared with control plants, only after growth at high light intensities. In both varieties the activities of FeSOD, ascorbate peroxidase, dehydroascorbate reductase, and monodehydroascorbate reductase and the concentrations of glutathione and ascorbate (all expressed on a chlorophyll basis) increased with increasing light intensity during growth. Most of these components were correlated with MV tolerance. It is argued that SOD overexpression leads to enhancement of the tolerance to MV-dependent oxidative stress only if one or more of these components is also present at high levels. Furthermore, the results suggest that in var SR1 the overexpressed MnSOD enhances primarily the stromal antioxidant system.
Keywords
ASCORBATE PEROXIDASE, GLUTATHIONE-REDUCTASE, HYDROGEN-PEROXIDE, PHOTOSYSTEM-II, SPINACH-CHLOROPLASTS, CONYZA-BONARIENSIS, OXYGEN, PLANTS, FERREDOXIN, MECHANISM

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MLA
Slooten, Luit, Katelijne Capiau, Wim Van Camp, et al. “Factors Affecting the Enhancement of Oxidative Stress Tolerance in Transgenic Tobacco Overexpressing Manganese Superoxide Dismutase in the Chloroplasts.” PLANT PHYSIOLOGY 107.3 (1995): 737–750. Print.
APA
Slooten, L., Capiau, K., Van Camp, W., Van Montagu, M., Sybesma, C., & Inzé, D. (1995). Factors affecting the enhancement of oxidative stress tolerance in transgenic tobacco overexpressing manganese superoxide dismutase in the chloroplasts. PLANT PHYSIOLOGY, 107(3), 737–750.
Chicago author-date
Slooten, Luit, Katelijne Capiau, Wim Van Camp, Marc Van Montagu, Chris Sybesma, and Dirk Inzé. 1995. “Factors Affecting the Enhancement of Oxidative Stress Tolerance in Transgenic Tobacco Overexpressing Manganese Superoxide Dismutase in the Chloroplasts.” Plant Physiology 107 (3): 737–750.
Chicago author-date (all authors)
Slooten, Luit, Katelijne Capiau, Wim Van Camp, Marc Van Montagu, Chris Sybesma, and Dirk Inzé. 1995. “Factors Affecting the Enhancement of Oxidative Stress Tolerance in Transgenic Tobacco Overexpressing Manganese Superoxide Dismutase in the Chloroplasts.” Plant Physiology 107 (3): 737–750.
Vancouver
1.
Slooten L, Capiau K, Van Camp W, Van Montagu M, Sybesma C, Inzé D. Factors affecting the enhancement of oxidative stress tolerance in transgenic tobacco overexpressing manganese superoxide dismutase in the chloroplasts. PLANT PHYSIOLOGY. 1995;107(3):737–50.
IEEE
[1]
L. Slooten, K. Capiau, W. Van Camp, M. Van Montagu, C. Sybesma, and D. Inzé, “Factors affecting the enhancement of oxidative stress tolerance in transgenic tobacco overexpressing manganese superoxide dismutase in the chloroplasts,” PLANT PHYSIOLOGY, vol. 107, no. 3, pp. 737–750, 1995.
@article{192864,
  abstract     = {{Two varieties of tobacco (Nicotiana tabacum var PBD6 and var SR1) were used to generate transgenic lines overexpressing Mn-superoxide dismutase (MnSOD) in the chloroplasts. The overexpressed MnSOD suppresses the activity of those SODs (endogenous MnSOD and chloroplastic and cytosolic Cu/ZnSOD) that are prominent in young leaves but disappear largely or completely during aging of the leaves. The transgenic and control plants were grown at different light intensities and were then assayed for oxygen radical stress tolerance in leaf disc assays and for abundance of antioxidant enzymes and substrates in leaves. Transgenic plants had an enhanced resistance to methylviologen (MV), compared with control plants, only after growth at high light intensities. In both varieties the activities of FeSOD, ascorbate peroxidase, dehydroascorbate reductase, and monodehydroascorbate reductase and the concentrations of glutathione and ascorbate (all expressed on a chlorophyll basis) increased with increasing light intensity during growth. Most of these components were correlated with MV tolerance. It is argued that SOD overexpression leads to enhancement of the tolerance to MV-dependent oxidative stress only if one or more of these components is also present at high levels. Furthermore, the results suggest that in var SR1 the overexpressed MnSOD enhances primarily the stromal antioxidant system.}},
  author       = {{Slooten, Luit and Capiau, Katelijne and Van Camp, Wim and Van Montagu, Marc and Sybesma, Chris and Inzé, Dirk}},
  issn         = {{0032-0889}},
  journal      = {{PLANT PHYSIOLOGY}},
  keywords     = {{ASCORBATE PEROXIDASE,GLUTATHIONE-REDUCTASE,HYDROGEN-PEROXIDE,PHOTOSYSTEM-II,SPINACH-CHLOROPLASTS,CONYZA-BONARIENSIS,OXYGEN,PLANTS,FERREDOXIN,MECHANISM}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{737--750}},
  title        = {{Factors affecting the enhancement of oxidative stress tolerance in transgenic tobacco overexpressing manganese superoxide dismutase in the chloroplasts}},
  url          = {{http://dx.doi.org/10.1104/pp.107.3.737}},
  volume       = {{107}},
  year         = {{1995}},
}

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