Tumor necrosis factor induces apoptosis (programmed cell death) in normal endothelial cells in vitro
- Author
- Bernard Robaye, Roger Mosselmans, Walter Fiers, Jacques E Dumont and Paul Galand
- Organization
- Abstract
- Tumor necrosis factor (TNF) is cytotoxic for many tumoral cell lines, whereas normal cells generally are considered resistant to this action. This study shows that this cytokine causes massive death of bovine endothelial cells in primary culture in a concentration- and time-dependent manner. Dying cells exhibit all the ultrastructural changes and the internucleosome cleavage of DNA associated with apoptosis or 'programmed cell death.' This is the first report clearly showing a direct toxicity of TNF on endothelial cells and demonstrating that this results from the induction of the program of apoptotic death. Our observation raises the possibility that hemorrhagic necrosis in vivo, after treatment with TNF, might involve a direct cytocidal action on endothelial cells of the tumor neovasculature.
- Keywords
- FACTOR-ALPHA, DNA FRAGMENTATION, CYTO-TOXICITY, LYMPHOTOXIN, RABBIT, ACTIVATION, MODULATION, INTERLEUKIN-1, GROWTH
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-1925417
- MLA
- Robaye, Bernard, et al. “Tumor Necrosis Factor Induces Apoptosis (Programmed Cell Death) in Normal Endothelial Cells in Vitro.” AMERICAN JOURNAL OF PATHOLOGY, vol. 138, no. 2, 1991, pp. 447–53.
- APA
- Robaye, B., Mosselmans, R., Fiers, W., Dumont, J. E., & Galand, P. (1991). Tumor necrosis factor induces apoptosis (programmed cell death) in normal endothelial cells in vitro. AMERICAN JOURNAL OF PATHOLOGY, 138(2), 447–453.
- Chicago author-date
- Robaye, Bernard, Roger Mosselmans, Walter Fiers, Jacques E Dumont, and Paul Galand. 1991. “Tumor Necrosis Factor Induces Apoptosis (Programmed Cell Death) in Normal Endothelial Cells in Vitro.” AMERICAN JOURNAL OF PATHOLOGY 138 (2): 447–53.
- Chicago author-date (all authors)
- Robaye, Bernard, Roger Mosselmans, Walter Fiers, Jacques E Dumont, and Paul Galand. 1991. “Tumor Necrosis Factor Induces Apoptosis (Programmed Cell Death) in Normal Endothelial Cells in Vitro.” AMERICAN JOURNAL OF PATHOLOGY 138 (2): 447–453.
- Vancouver
- 1.Robaye B, Mosselmans R, Fiers W, Dumont JE, Galand P. Tumor necrosis factor induces apoptosis (programmed cell death) in normal endothelial cells in vitro. AMERICAN JOURNAL OF PATHOLOGY. 1991;138(2):447–53.
- IEEE
- [1]B. Robaye, R. Mosselmans, W. Fiers, J. E. Dumont, and P. Galand, “Tumor necrosis factor induces apoptosis (programmed cell death) in normal endothelial cells in vitro,” AMERICAN JOURNAL OF PATHOLOGY, vol. 138, no. 2, pp. 447–453, 1991.
@article{1925417,
abstract = {{Tumor necrosis factor (TNF) is cytotoxic for many tumoral cell lines, whereas normal cells generally are considered resistant to this action. This study shows that this cytokine causes massive death of bovine endothelial cells in primary culture in a concentration- and time-dependent manner. Dying cells exhibit all the ultrastructural changes and the internucleosome cleavage of DNA associated with apoptosis or 'programmed cell death.' This is the first report clearly showing a direct toxicity of TNF on endothelial cells and demonstrating that this results from the induction of the program of apoptotic death. Our observation raises the possibility that hemorrhagic necrosis in vivo, after treatment with TNF, might involve a direct cytocidal action on endothelial cells of the tumor neovasculature.}},
author = {{Robaye, Bernard and Mosselmans, Roger and Fiers, Walter and Dumont, Jacques E and Galand, Paul}},
issn = {{0002-9440}},
journal = {{AMERICAN JOURNAL OF PATHOLOGY}},
keywords = {{FACTOR-ALPHA,DNA FRAGMENTATION,CYTO-TOXICITY,LYMPHOTOXIN,RABBIT,ACTIVATION,MODULATION,INTERLEUKIN-1,GROWTH}},
language = {{eng}},
number = {{2}},
pages = {{447--453}},
title = {{Tumor necrosis factor induces apoptosis (programmed cell death) in normal endothelial cells in vitro}},
url = {{http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1886201/}},
volume = {{138}},
year = {{1991}},
}