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Characterization of the murine IL-5 receptor complex with the use of a panel of monoclonal antibodies: relationship to the murine IL-3 receptor

José Van der Heyden, Réné Devos, Geert Plaetinck, Ina Fache, Walter Fiers UGent and Jan Tavernier UGent (1991) JOURNAL OF IMMUNOLOGY. 147(10). p.3413-3418
abstract
To obtain mAb against the murine IL-5R (mIL-5R), Wistar rats were immunized with B13 cells, a murine Ly-1+ (CD5+) pre-B cell line which is dependent on IL-3 or IL-5 for its growth. A first group of six mAb could immunoprecipitate, from detergent-lysed B13 cells, a 60-kDa polypeptide (p60) corresponding to the recently cloned mIL-5R alpha-chain. A second group of three mAb was able to immunoprecipitate a protein doublet of 130 to 140 kDa (p130 and p140) corresponding to the previously characterized mIL-3R and mIL-3R-like polypeptide, respectively. One mAb (25C9) specifically bound the p130 polypeptide only. Here we show that: 1) mAb directed against the mIL-5R p60 component completely block IL-5 binding; 2) mAb recognizing the p130-p140 doublet interfere with both IL-3 and IL-5 binding; 3) mAb recognizing p130-p140 block the high affinity binding of IL-5 and hence the high affinity mIL-5R consists of the association of the p60 and p130 and/or p140 component; 4) one particular mAb, 25C9, which binds only to the p130 polypeptide, interferes with only IL-3 binding, and has no effect on the binding of IL-5. These results on binding were corroborated by a biologic assay based on the cytokine-dependent proliferation of B13 cells. The results presented here support a model for the mIL-5R consisting of the alpha-chain (p60) associated with the p140 (IL-3R-like), whereas the p130 (IL-3R) is not involved in the IL-5R complex.
Please use this url to cite or link to this publication:
author
organization
alternative title
Characterization of the murine IL-5 receptor complex with the use of a panel of monoclonal-antibodies : relationship to the murine IL-3 receptor
year
type
journalArticle (original)
publication status
published
subject
keyword
CELL-LINES, EOSINOPHIL DIFFERENTIATION FACTOR, GENE FAMILY, SELECTIVE ACTIVATOR, GROWTH, EXPRESSION, CLONING, PROLIFERATION, RECOMBINANT HUMAN INTERLEUKIN-5, COLONY-STIMULATING FACTOR
journal title
JOURNAL OF IMMUNOLOGY
J. Immunol.
volume
147
issue
10
pages
3413 - 3418
Web of Science type
Article
ISSN
0022-1767
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1925265
handle
http://hdl.handle.net/1854/LU-1925265
alternative location
http://www.jimmunol.org/content/147/10/3413.abstract
date created
2011-10-13 11:16:04
date last changed
2011-12-02 13:48:13
@article{1925265,
  abstract     = {To obtain mAb against the murine IL-5R (mIL-5R), Wistar rats were immunized with B13 cells, a murine Ly-1+ (CD5+) pre-B cell line which is dependent on IL-3 or IL-5 for its growth. A first group of six mAb could immunoprecipitate, from detergent-lysed B13 cells, a 60-kDa polypeptide (p60) corresponding to the recently cloned mIL-5R alpha-chain. A second group of three mAb was able to immunoprecipitate a protein doublet of 130 to 140 kDa (p130 and p140) corresponding to the previously characterized mIL-3R and mIL-3R-like polypeptide, respectively. One mAb (25C9) specifically bound the p130 polypeptide only. Here we show that: 1) mAb directed against the mIL-5R p60 component completely block IL-5 binding; 2) mAb recognizing the p130-p140 doublet interfere with both IL-3 and IL-5 binding; 3) mAb recognizing p130-p140 block the high affinity binding of IL-5 and hence the high affinity mIL-5R consists of the association of the p60 and p130 and/or p140 component; 4) one particular mAb, 25C9, which binds only to the p130 polypeptide, interferes with only IL-3 binding, and has no effect on the binding of IL-5. These results on binding were corroborated by a biologic assay based on the cytokine-dependent proliferation of B13 cells. The results presented here support a model for the mIL-5R consisting of the alpha-chain (p60) associated with the p140 (IL-3R-like), whereas the p130 (IL-3R) is not involved in the IL-5R complex.},
  author       = {Van der Heyden, Jos{\'e} and Devos, R{\'e}n{\'e} and Plaetinck, Geert and Fache, Ina and Fiers, Walter and Tavernier, Jan},
  issn         = {0022-1767},
  journal      = {JOURNAL OF IMMUNOLOGY},
  keyword      = {CELL-LINES,EOSINOPHIL DIFFERENTIATION FACTOR,GENE FAMILY,SELECTIVE ACTIVATOR,GROWTH,EXPRESSION,CLONING,PROLIFERATION,RECOMBINANT HUMAN INTERLEUKIN-5,COLONY-STIMULATING FACTOR},
  language     = {eng},
  number       = {10},
  pages        = {3413--3418},
  title        = {Characterization of the murine IL-5 receptor complex with the use of a panel of monoclonal antibodies: relationship to the murine IL-3 receptor},
  url          = {http://www.jimmunol.org/content/147/10/3413.abstract},
  volume       = {147},
  year         = {1991},
}

Chicago
Van der Heyden, José, Réné Devos, Geert Plaetinck, Ina Fache, Walter Fiers, and Jan Tavernier. 1991. “Characterization of the Murine IL-5 Receptor Complex with the Use of a Panel of Monoclonal Antibodies: Relationship to the Murine IL-3 Receptor.” Journal of Immunology 147 (10): 3413–3418.
APA
Van der Heyden, José, Devos, R., Plaetinck, G., Fache, I., Fiers, W., & Tavernier, J. (1991). Characterization of the murine IL-5 receptor complex with the use of a panel of monoclonal antibodies: relationship to the murine IL-3 receptor. JOURNAL OF IMMUNOLOGY, 147(10), 3413–3418.
Vancouver
1.
Van der Heyden J, Devos R, Plaetinck G, Fache I, Fiers W, Tavernier J. Characterization of the murine IL-5 receptor complex with the use of a panel of monoclonal antibodies: relationship to the murine IL-3 receptor. JOURNAL OF IMMUNOLOGY. 1991;147(10):3413–8.
MLA
Van der Heyden, José, Réné Devos, Geert Plaetinck, et al. “Characterization of the Murine IL-5 Receptor Complex with the Use of a Panel of Monoclonal Antibodies: Relationship to the Murine IL-3 Receptor.” JOURNAL OF IMMUNOLOGY 147.10 (1991): 3413–3418. Print.