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Assessment of the antiviral properties of recombinant porcine SP-D against various influenza A viruses in vitro

(2011) PLOS ONE. 6(9).
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Abstract
The emergence of influenza viruses resistant to existing classes of antiviral drugs raises concern and there is a need for novel antiviral agents that could be used therapeutically or prophylacticaly. Surfactant protein D (SP-D) belongs to the family of C-type lectins which are important effector molecules of the innate immune system with activity against bacteria and viruses, including influenza viruses. In the present study we evaluated the potential of recombinant porcine SP-D as an antiviral agent against influenza A viruses (IAVs) in vitro. To determine the range of antiviral activity, thirty IAVs of the subtypes H1N1, H3N2 and H5N1 that originated from birds, pigs and humans were selected and tested for their sensitivity to recombinant SP-D. Using these viruses it was shown by hemagglutination inhibition assay, that recombinant porcine SP-D was more potent than recombinant human SP-D and that especially higher order oligomeric forms of SP-D had the strongest antiviral activity. Porcine SP-D was active against a broad range of IAV strains and neutralized a variety of H1N1 and H3N2 IAVs, including 2009 pandemic H1N1 viruses. Using tissue sections of ferret and human trachea, we demonstrated that recombinant porcine SP-D prevented attachment of human seasonal H1N1 and H3N2 virus to receptors on epithelial cells of the upper respiratory tract. It was concluded that recombinant porcine SP-D holds promise as a novel antiviral agent against influenza and further development and evaluation in vivo seems warranted.
Keywords
AMANTADINE, CELLS, RESISTANCE, SURVEILLANCE, ATTACHMENT, INFECTION, HEMAGGLUTININ, LOWER RESPIRATORY-TRACT, SURFACTANT PROTEIN-D, ISOLATED WORLDWIDE

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Chicago
Hillaire, Marine LB, Martin van Eijk, Stella E van Trierum, Debby van Riel, Xavier Saelens, Roland A Romijn, Wieger Hemrika, et al. 2011. “Assessment of the Antiviral Properties of Recombinant Porcine SP-D Against Various Influenza A Viruses in Vitro.” Plos One 6 (9).
APA
Hillaire, M. L., van Eijk, M., van Trierum, S. E., van Riel, D., Saelens, X., Romijn, R. A., Hemrika, W., et al. (2011). Assessment of the antiviral properties of recombinant porcine SP-D against various influenza A viruses in vitro. PLOS ONE, 6(9).
Vancouver
1.
Hillaire ML, van Eijk M, van Trierum SE, van Riel D, Saelens X, Romijn RA, et al. Assessment of the antiviral properties of recombinant porcine SP-D against various influenza A viruses in vitro. PLOS ONE. 2011;6(9).
MLA
Hillaire, Marine LB, Martin van Eijk, Stella E van Trierum, et al. “Assessment of the Antiviral Properties of Recombinant Porcine SP-D Against Various Influenza A Viruses in Vitro.” PLOS ONE 6.9 (2011): n. pag. Print.
@article{1924092,
  abstract     = {The emergence of influenza viruses resistant to existing classes of antiviral drugs raises concern and there is a need for novel antiviral agents that could be used therapeutically or prophylacticaly. Surfactant protein D (SP-D) belongs to the family of C-type lectins which are important effector molecules of the innate immune system with activity against bacteria and viruses, including influenza viruses. In the present study we evaluated the potential of recombinant porcine SP-D as an antiviral agent against influenza A viruses (IAVs) in vitro. To determine the range of antiviral activity, thirty IAVs of the subtypes H1N1, H3N2 and H5N1 that originated from birds, pigs and humans were selected and tested for their sensitivity to recombinant SP-D. Using these viruses it was shown by hemagglutination inhibition assay, that recombinant porcine SP-D was more potent than recombinant human SP-D and that especially higher order oligomeric forms of SP-D had the strongest antiviral activity. Porcine SP-D was active against a broad range of IAV strains and neutralized a variety of H1N1 and H3N2 IAVs, including 2009 pandemic H1N1 viruses. Using tissue sections of ferret and human trachea, we demonstrated that recombinant porcine SP-D prevented attachment of human seasonal H1N1 and H3N2 virus to receptors on epithelial cells of the upper respiratory tract. It was concluded that recombinant porcine SP-D holds promise as a novel antiviral agent against influenza and further development and evaluation in vivo seems warranted.},
  articleno    = {e25005},
  author       = {Hillaire, Marine LB and van Eijk, Martin and van Trierum, Stella E and van Riel, Debby and Saelens, Xavier and Romijn, Roland A and Hemrika, Wieger and Fouchier, Ron AM and Kuiken, Thijs and Osterhaus, Albert DME and Haagsman, Henk P and Rimmelzwaan, Guus F},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {AMANTADINE,CELLS,RESISTANCE,SURVEILLANCE,ATTACHMENT,INFECTION,HEMAGGLUTININ,LOWER RESPIRATORY-TRACT,SURFACTANT PROTEIN-D,ISOLATED WORLDWIDE},
  language     = {eng},
  number       = {9},
  pages        = {11},
  title        = {Assessment of the antiviral properties of recombinant porcine SP-D against various influenza A viruses in vitro},
  url          = {http://dx.doi.org/10.1371/journal.pone.0025005},
  volume       = {6},
  year         = {2011},
}

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