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Sporosarcina newyorkensis sp. nov. from clinical specimens and raw cow's milk

William J Wolfgang, An Coorevits UGent, Jocelyn A Cole, Paul De Vos UGent, Michelle C Dickinson, George E Hannett, Reashma Jose, Elizabeth J Nazarian, Peter Schumann and Anita Van Landschoot UGent, et al. (2012) INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 62(2). p.322-329
abstract
Twelve independent isolates of a Gram-positive, endospore-forming rod were recovered from clinical specimens in New York State, U.S.A and from raw milk in Flanders, Belgium. The 16S rRNA gene sequences for all isolates were identical. The closest species with a validly published name, based on 16S rRNA gene sequence, is Sporosarcina koreensis (97.13% similarity). DNA-DNA hybridization studies demonstrate that the new isolates belong to a species distinct from their nearest phylogenetic neighbors. The partial sequence of the 23S rRNA gene for the new strains and their nearest neighbors also provide support for the new species designation. Maximum-likelihood phylogenetic analysis of the 16S rRNA gene sequences confirmed that the new isolates are in the genus Sporosarcina. The predominant menaquinone is MK-7, the peptidoglycan has the type A4α L-Lys-Gly-D-Glu, and the polar lipids consist of diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The predominant fatty acids are iso-C(14:0), iso-C(15:0), and anteiso-C(15:0). In addition, biochemical and morphological analyses support designation of the twelve isolates as representatives of a single new species within the genus Sporosarcina, for which the name Sporosarcina newyorkensis sp. nov. (type strain 6062(T) =DSM 23544(T) =CCUG 59649(T) =LMG 26022(T)) is proposed.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Clinical isolate, Raw milk isolate, Sporosarcina sp. nov., DNA HYBRIDIZATION, COMB. NOV, DEOXYRIBONUCLEIC-ACID, RENATURATION RATES, LIPID-COMPOSITION, FORMING BACTERIA, GENUS BACILLUS, ET-AL., STRAINS, RECLASSIFICATION
journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Int. J. Syst. Evol. Microbiol.
volume
62
issue
2
pages
322 - 329
Web of Science type
Article
Web of Science id
000300861600010
JCR category
MICROBIOLOGY
JCR impact factor
2.112 (2012)
JCR rank
68/116 (2012)
JCR quartile
3 (2012)
ISSN
1466-5026
DOI
10.1099/ijs.0.030080-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1919948
handle
http://hdl.handle.net/1854/LU-1919948
date created
2011-10-03 16:32:31
date last changed
2012-10-03 17:14:44
@article{1919948,
  abstract     = {Twelve independent isolates of a Gram-positive, endospore-forming rod were recovered from clinical specimens in New York State, U.S.A and from raw milk in Flanders, Belgium. The 16S rRNA gene sequences for all isolates were identical. The closest species with a validly published name, based on 16S rRNA gene sequence, is Sporosarcina koreensis (97.13\% similarity). DNA-DNA hybridization studies demonstrate that the new isolates belong to a species distinct from their nearest phylogenetic neighbors. The partial sequence of the 23S rRNA gene for the new strains and their nearest neighbors also provide support for the new species designation. Maximum-likelihood phylogenetic analysis of the 16S rRNA gene sequences confirmed that the new isolates are in the genus Sporosarcina. The predominant menaquinone is MK-7, the peptidoglycan has the type A4\ensuremath{\alpha} L-Lys-Gly-D-Glu, and the polar lipids consist of diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The predominant fatty acids are iso-C(14:0), iso-C(15:0), and anteiso-C(15:0). In addition, biochemical and morphological analyses support designation of the twelve isolates as representatives of a single new species within the genus Sporosarcina, for which the name Sporosarcina newyorkensis sp. nov. (type strain 6062(T) =DSM 23544(T) =CCUG 59649(T) =LMG 26022(T)) is proposed.},
  author       = {Wolfgang, William J and Coorevits, An and Cole, Jocelyn A and De Vos, Paul and Dickinson, Michelle C and Hannett, George E and Jose, Reashma and Nazarian, Elizabeth J and Schumann, Peter and Van Landschoot, Anita and Wirth, Samantha E and Musser, Kimberlee A},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {Clinical isolate,Raw milk isolate,Sporosarcina sp. nov.,DNA HYBRIDIZATION,COMB. NOV,DEOXYRIBONUCLEIC-ACID,RENATURATION RATES,LIPID-COMPOSITION,FORMING BACTERIA,GENUS BACILLUS,ET-AL.,STRAINS,RECLASSIFICATION},
  language     = {eng},
  number       = {2},
  pages        = {322--329},
  title        = {Sporosarcina newyorkensis sp. nov. from clinical specimens and raw cow's milk},
  url          = {http://dx.doi.org/10.1099/ijs.0.030080-0},
  volume       = {62},
  year         = {2012},
}

Chicago
Wolfgang, William J, An Coorevits, Jocelyn A Cole, Paul De Vos, Michelle C Dickinson, George E Hannett, Reashma Jose, et al. 2012. “Sporosarcina Newyorkensis Sp. Nov. from Clinical Specimens and Raw Cow’s Milk.” International Journal of Systematic and Evolutionary Microbiology 62 (2): 322–329.
APA
Wolfgang, W. J., Coorevits, A., Cole, J. A., De Vos, P., Dickinson, M. C., Hannett, G. E., Jose, R., et al. (2012). Sporosarcina newyorkensis sp. nov. from clinical specimens and raw cow’s milk. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 62(2), 322–329.
Vancouver
1.
Wolfgang WJ, Coorevits A, Cole JA, De Vos P, Dickinson MC, Hannett GE, et al. Sporosarcina newyorkensis sp. nov. from clinical specimens and raw cow’s milk. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2012;62(2):322–9.
MLA
Wolfgang, William J, An Coorevits, Jocelyn A Cole, et al. “Sporosarcina Newyorkensis Sp. Nov. from Clinical Specimens and Raw Cow’s Milk.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 62.2 (2012): 322–329. Print.