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Characterization and purification of the F17 adhesin on the surface of bovine enteropathogenic and septicemic Escherichia coli

Paul F Lintermans, Pierre Pohl, Anne Bertels, Gerard Charlier, Joël Vandekerckhove UGent, Jozef Van Damme UGent, Johan Schoup, Christine Schlicker, Timo Korhonen and Henri De Greve, et al. (1988) AMERICAN JOURNAL OF VETERINARY RESEARCH. 49(11). p.1794-1799
abstract
The F17 antigen from bovine enterotoxigenic Escherichia coli strain (E coli 25KHO9), which adhered to calf intestinal villi, was isolated. An enterotoxin-negative derivative (25KHO9st) was used for further studies. Using an immunogold-labeling technique, the F17 antigen was characterized as a fimbrial protein. Pure fimbriae with a subunit molecular weight of 20,000 were obtained by homogenization and use of a sucrose gradient. The adhesion of E coli 25KHO9st was mediated by the F17 fimbriae, as both F17 antibodies and F17 protein blocked the adhesion of the strain 25KHO9st. The F17 fimbriae were serologically distinct from K88, K99, F41, and 987P fimbriae and did not agglutinate bovine, ovine, guinea pig, human, or chicken erythrocytes. Peptide fingerprint analysis revealed F17 and F(Y) adhesins to be homologous, if not identical.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
AMERICAN JOURNAL OF VETERINARY RESEARCH
Am. J. Vet. Res.
volume
49
issue
11
pages
1794 - 1799
Web of Science type
Article
ISSN
0002-9645
language
English
UGent publication?
yes
classification
A1
id
1918784
handle
http://hdl.handle.net/1854/LU-1918784
date created
2011-09-29 15:59:43
date last changed
2011-10-18 10:45:01
@article{1918784,
  abstract     = {The F17 antigen from bovine enterotoxigenic Escherichia coli strain (E coli 25KHO9), which adhered to calf intestinal villi, was isolated. An enterotoxin-negative derivative (25KHO9st) was used for further studies. Using an immunogold-labeling technique, the F17 antigen was characterized as a fimbrial protein. Pure fimbriae with a subunit molecular weight of 20,000 were obtained by homogenization and use of a sucrose gradient. The adhesion of E coli 25KHO9st was mediated by the F17 fimbriae, as both F17 antibodies and F17 protein blocked the adhesion of the strain 25KHO9st. The F17 fimbriae were serologically distinct from K88, K99, F41, and 987P fimbriae and did not agglutinate bovine, ovine, guinea pig, human, or chicken erythrocytes. Peptide fingerprint analysis revealed F17 and F(Y) adhesins to be homologous, if not identical.},
  author       = {Lintermans, Paul F and Pohl, Pierre and Bertels, Anne and Charlier, Gerard and Vandekerckhove, Jo{\"e}l and Van Damme, Jozef and Schoup, Johan and Schlicker, Christine and Korhonen, Timo and De Greve, Henri and Van Montagu, Marc},
  issn         = {0002-9645},
  journal      = {AMERICAN JOURNAL OF VETERINARY RESEARCH},
  language     = {eng},
  number       = {11},
  pages        = {1794--1799},
  title        = {Characterization and purification of the F17 adhesin on the surface of bovine enteropathogenic and septicemic Escherichia coli},
  volume       = {49},
  year         = {1988},
}

Chicago
Lintermans, Paul F, Pierre Pohl, Anne Bertels, Gerard Charlier, Joël Vandekerckhove, Jozef Van Damme, Johan Schoup, et al. 1988. “Characterization and Purification of the F17 Adhesin on the Surface of Bovine Enteropathogenic and Septicemic Escherichia Coli.” American Journal of Veterinary Research 49 (11): 1794–1799.
APA
Lintermans, P. F., Pohl, P., Bertels, A., Charlier, G., Vandekerckhove, J., Van Damme, J., Schoup, J., et al. (1988). Characterization and purification of the F17 adhesin on the surface of bovine enteropathogenic and septicemic Escherichia coli. AMERICAN JOURNAL OF VETERINARY RESEARCH, 49(11), 1794–1799.
Vancouver
1.
Lintermans PF, Pohl P, Bertels A, Charlier G, Vandekerckhove J, Van Damme J, et al. Characterization and purification of the F17 adhesin on the surface of bovine enteropathogenic and septicemic Escherichia coli. AMERICAN JOURNAL OF VETERINARY RESEARCH. 1988;49(11):1794–9.
MLA
Lintermans, Paul F, Pierre Pohl, Anne Bertels, et al. “Characterization and Purification of the F17 Adhesin on the Surface of Bovine Enteropathogenic and Septicemic Escherichia Coli.” AMERICAN JOURNAL OF VETERINARY RESEARCH 49.11 (1988): 1794–1799. Print.